An optimized method for the functional analysis of human regulatory T cells

Scandinavian Journal of Immunology

Volumn 64, Issue 3, 2006, Pages 353-360

  Oberg, H H ^a   Wesch, D ^a   Lenke, J ^a   Kabelitz, D ^a  

^a UNIVERSITY HOSPITAL SCHLESWIG HOLSTEIN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BASILIXIMAB; CD3 ANTIBODY; CYTOKINE; HYBRID PROTEIN; INTERLEUKIN 2; INTERLEUKIN 5; INTERLEUKIN 6; INTERLEUKIN 6 RECEPTOR; MONOCLONAL ANTIBODY CD28; TUMOR NECROSIS FACTOR RECEPTOR;

ARTICLE; CD4+ CD25+ T LYMPHOCYTE; CD4+ T LYMPHOCYTE; CELL CULTURE; CELL INTERACTION; CELL ISOLATION; CELL TYPE; CONCENTRATION (PARAMETERS); FUNCTIONAL ASSESSMENT; HUMAN; HUMAN CELL; LYMPHOCYTE ACTIVATION; LYMPHOCYTE CULTURE; LYMPHOCYTE FUNCTION; LYMPHOCYTE PROLIFERATION; NORMAL HUMAN; PRIORITY JOURNAL; REGULATORY T LYMPHOCYTE; REPRODUCIBILITY; T LYMPHOCYTE;

ANTIBODIES, MONOCLONAL; ANTIGENS, CD28; ANTIGENS, CD3; ANTIGENS, DIFFERENTIATION; CELL PROLIFERATION; CELL SEPARATION; HUMANS; IMMUNOMAGNETIC SEPARATION; LYMPHOCYTE ACTIVATION; T-LYMPHOCYTES; T-LYMPHOCYTES, REGULATORY;

EID: 33747082372     PISSN: 03009475     EISSN: 13653083     Source Type: Journal    
DOI: 10.1111/j.1365-3083.2006.01825.x     Document Type: Article

References (42)

1. Sakaguchi S, Sakaguchi N, Asano M, Ithoh M, Toda M. Immunologic self-tolerance maintained by activated T cells expressing IL-2 receptor α chains (CD25). Breakdown of a single mechanism of self-tolerance causes various autoimmune diseases. J Immunol 1995;155:1151-64.
2. + regulatory T cells for immunologic self-tolerance and negative control of immune responses. Annu Rev Immunol 2004;22:531-62.
3. Baecher-Allan C, Hafler DA. Suppressor T cells in human diseases. J Exp Med 2004;200:273-6.
4. + regulatory T cells. Trends Immunol 2004;25:375-80.
5. + regulatory T cells. J Immunol 2005;174:90-8.
6. Walsh PT, Taylor DK, Turka LA. Tregs and transplantation tolerance. J Clin Invest 2004;114:1398-403.
7. Belkaid Y, Rouse BT. Natural regulatory T cells in infectious disease. Nat Immunol 2005;6:353-60.
8. + T cells. Clin Immunol 2005;115:10-8.
9. + immunoregulatory T cells: gene expression analysis reveals a functional role for the glucocorticoid-induced TNF receptor. Immunity 2002;16:311-23.
10. Nocentini G, Riccardi C. GITR: a multifaceted regulator of immunity belonging to the tumor necrosis factor receptor superfamily. Eur J Immunol 2005;35:1016-22.
11. Jago CB, Yates J, Camara NOS, Lechler RI, Lombardi G. Differential expression of CTLA-4 among T cell subsets. Clin Exp Immunol 2004;136:463-71.
12. + regulatory T cells are dependent on CTLA-4 expression. Eur J Immunol 2004;34:3485-96.
13. Huang CT, Workman CJ, Flies D et al. Role of LAG-3 in regulatory T cells. Immunity 2004;21:503-13.
14. Bruder D, Probst-Kepper M, Westendorf AM et al. Neuropilin: a surface marker of regulatory T cells. Eur J Immunol 2004;34:623-30.
15. Kabelitz D, Oberg HH, Wesch D. Regulation of regulatory T cells: role of dendritic cells and Toll-like receptors. Crit Rev Immunol 2006 (in press).
16. Brunkow ME, Gardner JC, Hjerrild KA et al. Disruption of a new forkhead/winged-helix protein, scurfin, results in the fatal lymphoproliferative disorder of the scurfy mouse. Nat Genet 2001;27:68-73.
17. Bennett CL, Christie J, Ramsdell F et al. The immune dysregulation, polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) is caused by mutations of FOXP3. Nat Genet 2001;27:20-1.
18. Hori S, Nomura T, Sakaguchi S. Control of regulatory T cell development by the transcription factor Foxp3. Science 2003;299:1057-61.
19. + regulatory T cells. Nat Immunol 2003;4:337-42.
20. Schubert LA, Jeffery EJ, Zhang Y, Ramsdell F, Ziegler SF. Scurfin (FOXP3) acts as a repressor of transcription and regulates T cell activation. J Biol Chem 2001;276:37672-9.
21. Bettelli E, Dastrange M, Ouka M. Foxp3 interacts with nuclear factor of activated T cells and NF-κB to repress cytokine gene expression and effector functions of T helper cells. Proc Natl Acad Sci U S A 2005;102:5138-43.
22. Jonuleit H, Schmitt E. The regulatory T cell family: distinct subsets and their interrelations. J Immunol 2003;171:6323-7.
23. Höglund P. Induced peripheral regulatory T cells: the family grows larger. Eur J Immunol 2006;36:264-6.
24. + T cells with regulatory properties from human blood. J Exp Med 2001;193:1303-10.
25. + regulatory cell activation and function by dendritic cells. Int Immunol 2004;16:1769-80.
26. + T cell-mediated suppression by dendritic cells. Science 2003;299:1033-6.
27. Kubo T, Hatton RD, Oliver J, Liu X, Elson CO, Weaver CT. Regulatory T cell suppression and anergy are differentially regulated by proinflammatory cytokines produced by TLR-activated dendritic cells. J Immunol 2004;173:7249-58.
28. + T regulatory cells: crosstalk between two professionals in immunity versus tolerance. Front Biosci 2006;11:1360-70.
29. + regulatory T cell function. Science 2005;309:1380-4.
30. Sutmuller RP, den Brok MH, Kramer M et al. Toll-like receptor 2 controls expansion and function of regulatory T cells. J Clin Invest 2006;116:485-94.
31. + cells: a naturally occurring population of regulatory T cells. Blood 2001;98:2736-44.
32. + T cells: a highly differentiated and apoptosis-prone population. Eur J Immunol 2001;31:1122-31.
33. + regulatory T cells. Eur J Immunol 2002;32:1621-30.
34. + regulatory T cells and their ligands: implications for immunotherapy. Immunity 2004;20:107-18.
35. + T helper cells. J Exp Med 2002;196:255-60.
36. + regulatory, contact-dependent T cells induce interleukin-10 producing, contact-independent type-1 like regulatory T cells. J Exp Med 2002;196:247-53.
37. + regulatory T-cells. Methods Mol Med 2005;109:285-96.
38. Ichihara F, Kono K, Takahashi A, Kawaida H, Sugai H, Fuji H. Increased populations of regulatory T cells in peripheral blood and tumor-infiltrating lymphocytes in patients with gastric and esophageal cancers. Clin Cancer Res 2003;9:4404-8.
39. + cells in vitro. Am J Transpl 2005;5:454-64.
40. + T regulatory cells. J Immunol 2005;175:8051-9.
41. Gmeling-Meyling F, Ballieux RE. Simplified procedure for the separation of human T and non-T cells. Vox Sang 1977;33:5-12.
42. - and CD25 high regulatory T cells. Int Immunol 2006;18:555-63.