Probing the substrate specificity of the dengue virus type 2 NS3 serine protease by using internally quenched fluorescent peptides

Biochemical Journal

Volumn 397, Issue 1, 2006, Pages 203-211

  Niyomrattanakit, Pornwaratt ^a,b   Yahorava, Sviatlana ^b   Mutule, Ilze ^b   Mutulis, Felikss ^b   Petrovska, Ramona ^b   Prusis, Peteris ^b   Katzenmeier, Gerd ^a   Wikberg, Jarl E S ^b  

^a MAHIDOL UNIVERSITY   (Thailand)

^b UPPSALA UNIVERSITY   (Sweden)

Author keywords

3 nitrotyrosine; Dengue virus; Dengue virus non structural protein 3 (NS3) serine protease; Fluorescence; Polyprotein; Substrate specificity

Indexed keywords

AMINO ACIDS; BIOCHEMICAL ENGINEERING; FLUORESCENCE; POLYPEPTIDES; PROTEINS; QUENCHING; SUBSTRATES;

3-NITROTYROSINE; DENGUE VIRUS; DENGUE VIRUS NON-STRUCTURAL PROTEIN 3 (NS3) SERINE PROTEASE; POLYPROTEIN; SUBSTRATE SPECIFICITY;

VIRUSES;

3 NITROTYROSINE; AMINO ACID; ANTHRANILIC ACID; ANTIVIRUS AGENT; CAPSID PROTEIN; O AMINO BENZOIC ACID; POLYPROTEIN; SERINE PROTEINASE; UNCLASSIFIED DRUG; 3-NITROTYROSINE; ANTHRANILIC ACID DERIVATIVE; DRUG DERIVATIVE; NS3 PROTEIN, FLAVIVIRUS; PEPTIDE LIBRARY; RNA HELICASE; SERINE; TYROSINE; VIRUS PROTEIN;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CATALYST; CHEMICAL STRUCTURE; CONTROLLED STUDY; DENGUE VIRUS; FLUORESCENCE; HUMAN; HUMAN CELL; HYDROLYSIS; KINETICS; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; ESCHERICHIA COLI; METABOLISM;

DENGUE VIRUS; DENGUE VIRUS TYPE 2; MIRIDAE;

ANTHRANILIC ACIDS; DENGUE VIRUS; ESCHERICHIA COLI; FLUORESCENCE; PEPTIDE LIBRARY; RNA HELICASES; SERINE; SERINE ENDOPEPTIDASES; SUBSTRATE SPECIFICITY; TYROSINE; VIRAL NONSTRUCTURAL PROTEINS;

EID: 33745516142     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20051767     Document Type: Article

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