Heterogeneous expression of human cathelicidin hCAP18/LL-37 in inflammatory bowel diseases

European Journal of Gastroenterology and Hepatology

Volumn 18, Issue 6, 2006, Pages 615-621

  Schauber, Jürgen ^a,c,d   Rieger, Daniel ^a   Weiler, Frank ^a   Wehkamp, Jan ^b   Eck, Matthias ^a   Fellermann, Klaus ^b   Scheppach, Wolfgang ^a   Gallo, Richard L ^c   Stange, Eduard F ^b  

^a UNIVERSITY OF WÜRZBURG   (Germany)

^b ROBERT BOSCH HOSPITAL   (Germany)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d VA SAN DIEGO HEALTHCARE SYSTEM   (United States)

Author keywords

Antimicrobial peptides; Cathelicidin LL 37; Crohn's disease; Inflammatory bowel disease; Innate immunity; Ulcerative colitis

Indexed keywords

BUTYRIC ACID; CASPASE RECRUITMENT DOMAIN PROTEIN 15; CATHELICIDIN; CYTOKINE;

ADULT; ARTICLE; CELL STIMULATION; CLINICAL ARTICLE; COLON MUCOSA; CONTROLLED STUDY; CROHN DISEASE; ENTERITIS; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; INNATE IMMUNITY; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; ULCERATIVE COLITIS;

EID: 33744493110     PISSN: 0954691X     EISSN: None     Source Type: Journal    
DOI: 10.1097/00042737-200606000-00007     Document Type: Article

References (35)

1. Swidsinski A, Ladhoff A, Pernthaler A, Swidsinski S, Loening-Baucke V, Ortner M, et al. Mucosal flora in inflammatory bowel disease. Gastroenterology 2002; 122:44-54.
2. Campieri M, Gionchetti P. Bacteria as the cause of ulcerative colitis. Gut 2001; 48:132-135.
3. Elson CO, Sartor RB. Tennyson GS, Riddell RH. Experimental models of inflammatory bowel disease. Gastroenterology 1995; 109:1344-1367.
4. Duchmann R, May E, Heike M, Knolle R, Neurath M, Meyer zum Büschenfelde K-H. T cell specificity and cross reactivity towards enterobacteria, Bacteroides, Bifidobacterium, and antigens from resident intestinal flora in humans. Gut 1999; 44:812-818.
5. Darfeuille-Michaud A, Neut C, Barnich N, Lederman E, Di Martino P, Desreumaux P, et al. Presence of adherent Escherichia coli strains in ileal mucosa of patients with Crohn's disease. Gastroenterology 1998; 115:1405-1413.
6. O'Neil DA, Porter EM, Elewaut D, Anderson GM, Eckmann L, Ganz T, Kagnoff MF. Expression and regulation of the human beta-defensins hBD-1 and hBD-2 in intestinal epithelium. J Immunol 1999; 163:6718-6724.
7. Wehkamp J, Harder J, Weichenthal M, Mueller O, Herrlinger KR, Fellermann K, et al. Inducible and constitutive beta-defensins are differentially expressed in Crohn's disease and ulcerative colitis. Inflamm Bowel Dis 2003; 9:215-223.
8. Schauber J, Svanholm C, Termén S, Iffland K, Menzel T, Scheppach W, et al. The expression of the cathelicidin LL-37 is modulated by short-chain fatty acids in colonocytes: relevance of signalling pathways. Gut 2003; 52:743-751.
9. Boman HG. Antibacterial peptides: key components needed in immunity. Cell 1991; 65:205-207.
10. Bals R. Epithelial antimicrobial peptides in host defense against infection. Respir Res 2000; 1:141-150.
11. Zaiou M, Gallo RL. Cathelicidins, essential gene-encoded mammalian antibiotics. J Mol Med 2002; 80:549-561.
12. Agerberth B, Gunne H, Odeberg J, Kogner P, Boman HG, Gudmundsson GH. FALL-39, a putative human peptide antibiotic, is cysteine-free and expressed in bone marrow and testis. Proc Natl Acad Sci USA 1995; 92:195-199.
13. Cowland JB, Johnsen AH, Borregaard N. hCAP-18, a cathelin/pro-bactenecin- like protein of human neutrophil specific granules. FEBS Lett 1995; 368:173-176.
14. Mason DJ, Dybowski R, Larrick JW, Gant VA. Antimicrobial action of rabbit leukocyte CAP18 (106-137). Antimicrob Agents Chemother 1997; 41:624-629.
15. Agerberth B, Charo J, Werr J, Olsson B, Idali F, Lindbom L, et al. The human antimicrobial and chemotactic peptides LL-37 and alpha-defensins are expressed by specific lymphocyte and monocyte populations. Blood 2000; 96:3086-3093.
16. Bals R, Wang X, Zasloff M, Wilson JM. The peptide antibiotic LL-37/hCAP-18 is expressed in epithelia of the human lung where it has broad antimicrobial activity at the airway surface. Proc Natl Acad Sci USA 1998; 95:9541-9546.
17. Frohm Nilsson M, Sandstedt B, Sorensen O, Weber G, Borregaard N, Stahle-Backdahl M. The human cationic antimicrobial protein (hCAP18), a peptide antibiotic, is widely expressed in human squamous epithelia and colocalizes with interleukin-6. Infect Immun 1999; 67:2561-2566.
18. Schauber J, Iffland K, Frisch S, Kudlich T, Schmausser B, Eck M, et al. Histone-deacetylase inhibitors induce the expression of the cathelicidin LL-37 in human gastrointestinal cells. Mol Immunol 2004; 41:847-854.
19. Hase K, Murakami M, Mitsutoshi I, Cole SP, Horibe Y, Ohtake T, et al. Expression of LL-37 by human gastric epithelial cells as apotential host defense mechanism against Helicobacter pylori. Gastroenterology 2003; 125:1613-1625.
20. Koczulla R, von Degenfeld G, Kupatt C, Krotz F, Zahler S, Gloe T, et al. An angiogenic role for the human peptide antibiotic LL-37/hCAP-18. J Clin Invest 2003; 111:1665-1672.
21. Wehkamp J, Harder J, Weichenthal M, Schwab M, Schaffeler E, Schlee M, et al. NOD2 (CARD15) mutations in Crohn's disease are associated with diminished mucosal alpha-defensin expression. Gut 2004; 53:1658-1664.
22. Wehkamp J, Salzman NH, Porter E, Nuding S, Weichenthal M, Petras RE, et al. Reduced Paneth cell alpha-defensins in ileal Crohn's disease. Proc Natl Acad Sci USA 2005; 102:18129-18134.
23. Hase K, Eckmann L, Leopard JD, Varki N, Kagnoff MF. Cell differentiation is a key determinant of cathelicidin LL-37/human cationic antimicrobial protein 18 expression by human colon epithelium. Infect Immun 2002; 70:953-963.
24. Bustin SA. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol 2000; 25:169-193.
25. Islam D, Bandholtz L, Nilsson J, Wigzell H, Christensson B, Agerberth B, Gudmundsson G. Downregulation of bactericidal peptides in enteric infections: a novel immune escape mechanism with bacterial DNA as a potential regulator. Nat Med 2001; 7:180-185.
26. Monteleone I, Vavassori P, Biancone L, Monteleone G, Pallone F. Immunoregulation in the gut: success and failures in human disease. Gut 2002; 50 (suppl 3):60-64.
27. Ramaekers F, Huysmanns A, Moesker A, Kant A, Jap P, Herman C, Vooijs P. Monoclonal antibody to keratin filaments, specific for glandular epithelia and their tumors. Lab Invest 1983; 49:353-361.
28. Ong PY, Ohtake T, Brandt C, Strickland I, Boguniewicz M, Ganz T, et al. Endogenous antimicrobial peptides and skin infections in atopic dermatitis. N Engl J Med 2002; 347:1151-1160.
29. Pütsep K, Carlsson G, Boman H, Andersson M. Deficiency of antibacterial peptides in patients with morbus Kostmann: an observation study. Lancet 2002; 360:1144-1149.
30. Iimura M, Gallo RL, Hase K, Miyamoto Y, Eckmann L, Kagnoff MF. Cathelicidin mediates innate intestinal defense against colonization with epithelial adherent bacterial pathogens. J Immunol 2005; 174:4901-4907.
31. Wu H, Zhang G, Ross C, Blecha F. Cathelicidin gene expression in procine tissues: roles in ontogeny and tissue specificity. Infect Immun 1999; 67:439-442.
32. Bouma G, Strober W. The immunological and genetic basis of inflammatory bowel disease. Nat Rev Immunol 2003; 3:521-533.
33. Nomura I, Goleva E, Howell MD, Hamid QA, Ong PY, Hall CF, et al. Cytokine milieu of atopic dermatitis, as compared to psoriasis, skin prevents induction of innate immune response genes. J Immunol 2003; 171:3262-3269.
34. Zaiou M, Nizet V, Gallo RL. Antimicrobial and protease inhibitory functions of the human cathelicidin (hCAP18/LL-37) prosequence. J Invest Dermatol 2003; 120:810-816.
35. Murakami M, Lopez-Garcia B, Braff M, Dorschner RA, Gallo RL. Postsecretory processing generates multiple cathelicidins for enhanced topical antimicrobial defense. J Immunol 2004; 172:3070-3077.