Effect of osmotic pressure on the production of retroviral vectors: Enhancement in vector stability

Biotechnology and Bioengineering

Volumn 94, Issue 2, 2006, Pages 322-329

  Coroadinha, A S ^a   Silva, A C ^a   Pires, E ^a   Coelho, A ^a   Alves, P M ^a   Carrondo, M J T ^a,b  

^a INSTITUTO DE TECNOLOGIA QUÍMICA E BIOLÓGICA   (Portugal)

^b UNIVERSIDADE NOVA DE LISBOA   (Portugal)

Author keywords

Cholesterol; Osmolality; Phospholipids; Production; Retroviral vectors; Stability

Indexed keywords

CELL CULTURE; CELLS; CHOLESTEROL; FATTY ACIDS; OSMOSIS; PHOSPHOLIPIDS; PRODUCTION; STABILITY;

OSMOLALITY; RETROVIRAL STABILITY; RETROVIRAL VECTORS; VECTOR STABILITY;

VIRUSES;

CHOLESTEROL; FRUCTOSE; LIPID; PHOSPHOLIPID; RETROVIRUS VECTOR; SODIUM CHLORIDE; SORBITOL;

ARTICLE; CELL MEMBRANE; CONTROLLED STUDY; CULTURE MEDIUM; NONHUMAN; OSMOLALITY; OSMOTIC PRESSURE; VIRUS INFECTION;

CELL LINE; CHOLESTEROL; CULTURE MEDIA; FRUCTOSE; GENETIC VECTORS; HCT116 CELLS; HUMANS; OSMOLAR CONCENTRATION; OSMOTIC PRESSURE; PHOSPHOLIPIDS; RETROVIRIDAE; SODIUM CHLORIDE; SORBITOL;

MURINAE;

EID: 33744474819     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.20847     Document Type: Article

References (35)

1. Aloia RC, Tian H, Jensen FC. 1993. Lipid composition and fluidity of the human immunodeficiency virus envelope and host cell plasma membranes. Proc Natl Acad Sci USA 90(11):5181-5185.
2. Andreadis ST, Roth CM, Le Doux JM, Morgan JR, Yarmush ML. 1999. Large-scale processing of recombinant retroviruses for gene therapy. Biotechnol Prog 15(1):1-11.
3. Beer C, Meyer A, Muller K, Wirth M. 2003. The temperature stability of mouse retroviruses depends on the cholesterol levels of viral lipid shell and cellular plasma membrane. Virology 308(1):137-146.
4. Bligh EG, Dyer WJ. 1959. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37(8):911-917.
5. Cherlet M, Marc A. 1999. Hybridoma cell behaviour in continuous culture under hyperosmotic stress. Cytotechnology 29:71-84.
6. Clegg J, Gallo J, Gordon E. 1986. Some structural, biochemical and biophysical characteristics of L-929 cells growing in the presence of hyperosmotic sorbitol concentrations. Exp Cell Res 163(1):35-46.
7. Coroadinha AS, Ribeiro J, Roldão A, Cruz PE, Alves PM, Merten O-W, Carrondo MJT. 2006. Effect of medium sugar source on the production of retroviral vectors for gene therapy. Biotech Bioeng (in press).
8. Cosset FL, Takeuchi Y, Battini JL, Weiss RA, Collins MK. 1995. High-titer packaging cells producing recombinant retroviruses resistant to human serum. J Virol 69(12):7430-7436.
9. Cruz PE, Almeida JS, Murphy PN, Moreira JL, Carrondo MJ. 2000. Modeling retrovirus production for gene therapy 1. Determination Of optimal bioreaction mode and harvest strategy. Biotechnol Prog 16(2):213-221.
10. Cruz PE, Carmo M, Coroadinha AS, Bengala A, Gonçalves D, Teixeira M, Merten O-W, Genny-Fiamma C, Carrondo MJT. 2005. Retroviral vector stability: Inactivation kinetics and membrane properties. In: Goœdia F, Fussenegger M, editors. Animal cell technology meets genomics. Dordrecht, NL: Springer, pp 303-308.
11. Davis JL, Witt RM, Gross PR, Hokanson CA, Jungles S, Cohen LK, Danos O, Spratt SK. 1997. Retroviral particles produced from a stable human-derived packaging cell line transduce target cells with very high efficiencies. Hum Gene Ther 8(12):1459-1467.
12. Dunbar C, Kohn D. 1996. Retroviral mediated transfer of the cDNA for human glucocerebrosidase into hematopoietic stem cells of patients with Gaucher disease. A phase I study. Hum Gene Ther 7(2):231-253.
13. Guyader M, Kiyokawa E, Abrami L, Turelli P, Trono D. 2002. Role for human immunodeficiency virus type 1 membrane cholesterol in viral internalization. J Virol 76(20):10356-10364.
14. Gény-Fiamma C, Millot L, Rocca C, Danos O, Merten O-W. 2004. Optimization of the production of retroviral vectors: Influence of the sugar source. In: Yagasaki K, Miura Y, Hattori M, Nomura Y, editors. Animal cell technology: Basic and applied aspects. Dordrecht: Kluwer Academic Publisher, pp 89-97.
15. Harada S, Yusa K, Monde K, Akaike T, Maeda Y. 2005. Influence of membrane fluidity on human immunodeficiency virus type 1 entry. Biochem Biophys Res Commun 329(2):480-486.
16. Higashikawa F, Chang L. 2001. Kinetic analyses of stability of simple and complex retroviral vectors. Virology 280(1):124-131.
17. Kim NS, Lee GM. 2002. Response of recombinant Chinese hamster ovary cells to hyperosmotic pressure: Effect of Bcl-2 overexpression. J Biotechnol 95(3):237-248.
18. Le Doux JM, Davis HE, Morgan JR, Yarmush ML. 1999. Kinetics of retrovirus production and decay. Biotechnol Bioeng 63(6):654-662.
19. Lee MS, Lee GM. 2000. Hyperosmotic pressure enhances immunoglobulin transcription rates and secretion rates of KR12H-2 transfectoma. Biotechnol Bioeng 68(3):260-268.
20. Lee SG, Kim S, Robbins PD, Kim BG. 1996. Optimization of environmental factors for the production and handling of recombinant retrovirus. Appl Microbiol Biotechnol 45(4):477-483.
21. McTaggart S, Al-Rubeai M. 2000. Effects of culture parameters on the production of retroviral vectors by a human packaging cell line. Biotechnol Prog 16(5):859-865.
22. Metten OW. 2004. State-of-the-art of the production of retroviral vectors. J Gene Med 6(Suppl 1):S105-S124.
23. Merten OW, Cruz PE, Rochette C, Geny-Fiamma C, Bouquet C, Goncalves D, Danos O, Carrondo MJ. 2001a. Comparison of different bioreactor systems for the production of high titer retroviral vectors. Biotechnol Prog 17(2):326-335.
24. Merten OW, Landric L, Danos O. 2001b. Influence of the metabolic state of packaging cells on retroviral vector production. In: Merten OW, Mattanovich D, Lang C, Larsson G, Neubauer P, Porro D, Postma P, Teixeira de Mattos J, Cole J, editors. Recombinant protein production with prokaryotic and eukaryotic cells. A comparative view on host physiology. Dordrecht/NL: Kluwer Academic Press, pp 301-316.
25. Morita S, Kojima T, Kitamura T. 2000. Plat-E: An efficient and stable system for transient packaging of retroviruses. Gene Ther 7(12):1063-1066.
26. Mountain A. 2000. Gene therapy: The first decade. Trends Biotechnol 18(3):119-128.
27. Olejnik A, Grajek W, Marecik R. 2003. Effect of hyperosmolarity on recombinant protein productivity in baculovirus expression system. J Biotechnol 102(3):291-300.
28. Ory DS, Neugeboren BA, Mulligan RC. 1996. A stable human-derived packaging cell line for production of high titer retrovirus/vesicular stomatitis virus G pseudotypes. Proc Natl Acad Sci USA 93(21):11400-11406.
29. Paul RW, Morris D, Hess BW, Dunn J, Overell RW. 1993. Increased viral titer through concentration of viral harvests from retroviral packaging lines. Hum Gene Ther 4(5):609-615.
30. Pizzato M, Merten OW, Blair ED, Takeuchi Y. 2001. Development of a suspension packaging cell line for production of high titre, serum-resistant murine leukemia virus vectors. Gene Ther 8(10):737-745.
31. Stewart JC. 1980. Colorimetric determination of phospholipids with ammonium ferrothiocyanate. Anal Biochem 104(1):10-14.
32. Suomalainen M. 2002. Lipid rafts and assembly of enveloped viruses. Traffic 3(10):705-709.
33. Takagi M, Hayashi H, Yoshida T. 2000. The effect of osmolarity on metabolism and morphology in adhesion and suspension Chinese hamster ovary cells producing tissue plasminogen activator. Cytotechnology 32(3):171-179.
34. Takagi M, Moriyama T, Yoshida T. 2001. Effects of shifts up and down in osmotic pressure on production of tissue plasminogen activator by Chinese hamster ovary cells in suspension. J Biosci Bioeng 91(5):509-514.
35. Yoshinaka Y, Luftig RB. 1981. Inhibition of murine leukemia virus Pr65gag cleavage in vitro and in vivo by hypertonic medium. J Virol 37(3):1066-1070.