Effects of shifts up and down in osmotic pressure on production of tissue plasminogen activator by Chinese hamster ovary cells in suspension

Journal of Bioscience and Bioengineering

Volumn 91, Issue 5, 2001, Pages 509-514

  Takagi, Mutsumi ^a   Moriyama, Tetsuhiro ^a   Yoshida, Toshiomi ^a  

^a OSAKA UNIVERSITY   (Japan)

Author keywords

CHO cells; Osmotic pressure; Shift down; tPA production rate

Indexed keywords

CELLS; TISSUE;

TISSUE PLASMINOGEN ACTIVATORS;

OSMOSIS;

TISSUE PLASMINOGEN ACTIVATOR;

ANIMAL CELL; ARTICLE; CELL GROWTH; CELL SUSPENSION; CHO CELL; NONHUMAN; OSMOTIC PRESSURE; PROTEIN SYNTHESIS;

ANIMALIA; CRICETINAE; CRICETULUS GRISEUS;

EID: 0034977008     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1389-1723(01)80282-6     Document Type: Article

References (16)

1. Hybridoma growth and antibody production as a function of cell density and specific growth rate in perfusion culture
2. Growth and antibody production of hybridoma cells exposed to hyperosmotic stress
3. Enhanced specific antibody productivity of hybridomas resulting from hyperosmotic stress are cell line specific
4. Intracellular responses of productive hybridomas subjected to high osmotic pressure
5. Effects of abrupt and gradual osmotic stress on antibody production and content in hybridoma cells that differ in production kinetics
6. Evidence that glutamine not sugar is the major energy source for cultured HeLa cells
7. Lactate: A major product of glutamine metabolism by human diploid fibroblasts
8. Glutamine: A major energy source for cultured mammalian cells
9. Effect of medium osmolarity on hybridoma growth, metabolism, and antibody production
10. Hyper-stimulation of monoclonal antibody production by high osmolarity stress in eRDF medium
11. Metabolic flux change in hybridoma cells under high osmotic pressure
12. The effect of osmolarity on metabolism and morphology in adhesion and suspension Chinese hamster ovary cells producing tissue plasminogen activator
13. Enhanced monoclonal antibody production by gradual increase of osmotic pressure
14. Effects of high concentrations of energy sources and metabolites on suspension culture of Chinese hamster ovary cells producing tissue plasminogen activator
15. Utilization of osmoprotective compounds by hybridoma cells exposed to hyperosmotic stress
16. Hyperosmotic Hybridoma cell culture: Increased monoclonal antibody production with addition of glycine betaine