Effect of hyperosmolarity on recombinant protein productivity in baculovirus expression system

Journal of Biotechnology

Volumn 102, Issue 3, 2003, Pages 291-300

  Olejnik, A ^a   Grajek, W ^a   Marecik, R ^a  

^a POZNA UNIVERSITY OF LIFE SCIENCES   (Poland)

Author keywords

Baculovirus; Insect cell culture; Osmotic pressure; Recombinant protein

Indexed keywords

BACTERIA; CELL CULTURE; CELLS; GENETIC ENGINEERING; GLUCOSE; METABOLISM; OSMOSIS; SODIUM CHLORIDE; SYNTHESIS (CHEMICAL); THERMODYNAMICS; VIRUSES;

HYPEROSMOLARITY;

PROTEINS;

GLUCOSE; LACTIC ACID; NUCLEOPROTEIN; RECOMBINANT PROTEIN; SODIUM CHLORIDE; SODIUM ION;

ARTICLE; BACULOVIRUS EXPRESSION SYSTEM; FED BATCH CULTURE; FEEDING; FERMENTATION TECHNIQUE; GLUCOSE INTAKE; HYPEROSMOLARITY; INSECT CELL; NONHUMAN; NUTRITION; OSMOTIC STRESS; PRECURSOR; PRIORITY JOURNAL; PROTEIN SYNTHESIS;

INSECTA; UNIDENTIFIED BACULOVIRUS;

EID: 0037687997     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(03)00034-8     Document Type: Article

References (19)

1. Bedard C., Kamen R., Tom R., Massie B. Maximization of recombinant protein yield in the insect cell/baculovirus system by one-time addition of nutrients to high-density batch cultures. Cytotechnology. 15:1995;129-138.
2. Claeys, Uytterhaegen L., Buts B., Demeyer D. Quantification of beef myofibrillar proteins by SDS-PAGE. Meat Sci. 39:1995;177-193.
3. Donaldson M.S., Shuler M.L. Low-cost serum-free medium for the BTI-Tn5B1-4 insect cell line. Biotechnol. Prog. 14:1998;573-579.
4. Kultz D., Burg M. Evolution of osmotic stress signalling via map kinase cascades [review]. J. Exp. Biol. 201:(22):1998;3015-3021.
5. Kultz D., Chakravarty D. Hyperosmolality in the form of elevated NaCl but not urea causes DNA damage in murine kidney cells. Proc. Natl. Acad. Sci. USA. 98:(4):2001;1999-2004.
6. Kultz D., Chakravarty D. Maintenance of genomic integrity in mammalian kidney cells exposed to hyperosmotic stress. Comp. Biochem. Physiol. A-Mol. Integr. Physiol. 130:(3):2001;421-428.
7. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
8. Lin J., Takagi M., Qu I., Gao P., Yoshida T. Enhanced monoclonal antibody production by gradual increase of osmotic pressure. Cytotechnology. 29:1999;27-33.
9. Mendoca R.Z., Palomares L.A., Ramirez O.T. An insight into insect cell metabolism through selective nutrient manipulation. J. Biotechnol. 72:1999;61-75.
10. O'Reilly D.R., Miller L.K., Luckow V.A. Baculovirus Expression Vectors. 1994;Oxford University Press.
11. Oh S.K.W., Chua F.K.F., Choo A.B.H. Intracellular responses of productive hybridomas subjected to high osmotic pressure. Biotechnol. Bioeng. 46:1995;525-535.
12. Parnaud G., Corpet D.E., Gamet-Payrastre L. Cytostatic effect of polyethylene glycol on human colonic adenocarcinoma cells. Int. J. Cancer. 92:2001;63-69.
13. Raghunand N., Dale B.E. Alteration of glucose consumption kinetics with progression of baculovirus infection in Spodoptera frugiperda cells. Appl. Biochem. Biotechnol. 80:(3):1999;231-242.
14. Rosinski M., Reid S., Nielsen L.K. Osmolarity effects on observed insect cell size after baculovirus infection are avoided using growth medium for sample dilution. Biotechnol. Prog. 16:(5):2000;782-785.
15. Takagi M., Hayashi H., Yoshida T. Effects of osmolarity on metabolism and morphology in adhesion and suspension Chinese hamster ovary cells producing tissue plasminogen activator. Cytotechnology. 32:2000;171-179.
16. Takagi M., Moriyama T., Yoshida T. Effects of shifts up and down in osmotic pressure on production of tissue plasminogen activator by Chinese hamster ovary cells in suspension. J. Biosci. Bioeng. 91:(5):2001;509-514.
17. Tom R.L., Debanne M.T., Bedard C., Caron A.W., Massie B., Kamen A.A. Improved yields of the extracellular domain of the epidermal growth factor receptor produced using the baculovirus expression system by medium replacement following infection. Appl. Microbiol. Biotechnol. 44:1995;53-58.
18. Tramper J., Lier F.L.J., Kool M., Gooijer C.D., Vlak J.M. Production of (recombinant) baculoviruses in insect-cell bioreactors. Vardar-Sukan F.F., Sukan S. Recent Advances in Biotechnology. 1992;263-284 Kluwer Academic Publishers, The Netherlands.
19. Yancey P.H., Clark M.E., Hand S.C., Bowlus R.D., Somero G.N. Living with water stress: evolution of osmolyte systems. Science. 217:1982;1214-1222.