The effect of osmolarity on metabolism and morphology in adhesion and suspension chinese hamster ovary cells producing tissue plasminogen activator

Cytotechnology

Volumn 32, Issue 3, 2000, Pages 171-179

  Takagi, Mutsumi ^a   Hayashi, Hiromasa ^a   Yoshida, Toshiomi ^a  

^a OSAKA UNIVERSITY   (Japan)

Author keywords

Adherent CHO cells; CHO cells; Morphology of CHO cells; Osmolarity; Suspended CHO cells; Tissue plasminogen activator

Indexed keywords

CELL ADHESION; CELL GROWTH; CELL METABOLISM; CELL VOLUME; CHO CELL; GLUCOSE METABOLISM; GROWTH RATE; LACTIC ACID; OSMOLARITY; SUSPENSION CELL CULTURE; TISSUE PLASMINOGEN ACTIVATOR;

EID: 0034080155     PISSN: 09209069     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1008171921282     Document Type: Article

References (12)

1. Butt HJ, Wolff EK, Gould SA, Dixon NB, Peterson CM & Hansma PK (1990) Imaging cells with the atomic force microscope. J Struct Biol 105: 54-61.
2. Fernandez E, Vicente A, Zapata A, Brera B, Lozano JJ, Martinez C & Toribio ML (1994) Establishment and characterization of cloned human thymic epithelial cell lines. Analysis of adhesion molecule expression and cytokine production. Blood 83: 3245-3254.
3. + peritoneal B lymphocyte population. Cell Immunol 161: 50-60.
4. Hooker AD, Goldman MH, Markham NH, James DC, Ison AP, Bull AT, Strange PG, Salmon I, Baines AJ & Jenkins N (1995) N-glycans of recombinant human interferon-γ change during batch culture of Chinese hamster ovary cells. Biotech Bioeng 48: 639-648.
5. Tong JZ, Bernard O & Alvarez F (1990) Long-term culture of rat liver cell spheroids in hormonally defined media. Exp Cell Res 189: 87-92.
6. Tsuji T, Ogasawara H, Aoki Y, Tsurumaki Y & Kodama H (1996) Characterization of murine stromal cell clones established from bone marrow and spleen. Leukemia 10: 803-812.
7. Oh SKW, Chua FKF & Choo ABH (1995) Intracellular responses of productive hybridomas subjected to high osmolarity. Biotech Bioeng 46: 525-535.
8. Oyaas K, Ellingsen TE, Dyrset N & Levine DW (1994) Utilization of osmoprotective compounds by hybridoma cells exposed to hyperosmotic stress. Biotech Bioeng 43: 77-89.
9. Oyaas K, Ellingsen TE, Dyrset N & Levine DW (1994) Hyper osmotic hybridoma cell culture: Increased monoclonal antibody production with addition of glycine betaine. Biotech Bioeng 44: 991-998.
10. Ozturk SS & Palsson BO (1991) Effect of medium osmolarity on hybridoma growth, metabolism, and antibody production. Biotech Bioeng 37: 989-993.
11. - exchanger. Am J Physiol 268: C147-C153.
12. Sanford KK, Earle WR, Evans VJ, Waltz HK & Shannon JE (1950) The measurement of proliferation in tissue cultures by enumeration of cell nuclei. J Nat Canc Inst 11: 773-795.