Effects of culture parameters on the production of retroviral vectors by a human packaging cell line

Biotechnology Progress

Volumn 16, Issue 5, 2000, Pages 859-865

  McTaggart, S ^a   Al Rubeai, M ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

HUMAN GENE THERAPY; HUMAN PACKAGING CELL LINE; RETROVIRAL VECTORS; SERUM;

CELL CULTURE EQUIPMENT; CELL CULTURE MONITORING; CELL LINE; HALF LIFE TIME; HUMAN CELL; PACKAGING CELL; PH; RETROVIRAL VECTOR; TEMPERATURE; VIRUS CULTURE;

BIOASSAY; CELL IMMOBILIZATION; CELLS; OXYGEN; PH EFFECTS; TEMPERATURE; VIRUSES;

CELL CULTURE;

BLOOD; CELL CULTURE TECHNIQUES; CELL LINE; CULTURE MEDIA; GENETIC VECTORS; HEAT; HUMANS; HYDROGEN-ION CONCENTRATION; OXYGEN; RETROVIRIDAE;

UNIDENTIFIED RETROVIRUS;

EID: 0034281468     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp000078j     Document Type: Article

References (30)

1. Adenovirus vectors for gene delivery
2. Strategies for the isolation and purification of retroviral vectors for human gene therapy
3. Effect of serum concentration on retroviral vector production from the amphotropic ψ CRIP murine producer cells
4. High-titre packaging cells producing recombinant retroviruses resistant to human serum
5. Gene expression in mice after high efficiency retroviral-mediated gene transfer
6. Oxygenation of intensive cell-culture system
7. Lentiviruses as gene delivery vectors
8. B7, a new member of the Ig superfamily with unique expression on activated and neoplastic B cells
9. Improved titres of retroviral vectors from the human FLY-RD18 packaging cell line in serum- and protein-free medium
10. Production of retroviral vectors for gene therapy with the human packaging cell line FLYRD18
11. Gene therapy using retroviral vectors
12. The vector void in gene therapy
13. Optimised conditions for the production of recombinant amphotropic retroviral vector preparations
14. Synthesis, characterisation and anti-tumour activity of new chloroethylamine platinum complexes
15. Improved methods of retroviral vector transduction and production for gene therapy
16. Multistage production of Autographa californica nuclear polyhedrosis virus in insect cell cultures
17. Optimization of environmental factors for the production and handling of recombinant retrovirus
18. The role of the CD28 receptor during T cell responses to antigen
19. Biochemical recovery and purification of gene therapy vectors
20. Induction of apoptosis in oxygen-deprived cultures of hybridoma cells
21. Improved retroviral vectors for gene transfer and expression
22. Delivering therapeutic genes - Matching approach and application
23. Retroviral vectors in human gene therapy
24. The physicochemical factors that govern retovirus-mediated gene transfer
25. Therapeutic delivery issues in gene therapy, Part 1: Vectors
26. Viral vectors for gene therapy
27. Hybridoma growth, metabolism, and product formation in HEPES-buffered medium: II. Effect of pH
28. Kinetics of retroviral production from the amphotropic CRIP murine producer cell line
29. Sensitization of cells and retroviruses to human serum by(α-1-3) galactosyltransferase