Comparison of different bioreactor systems for the production of high titer retroviral vectors

Biotechnology Progress

Volumn 17, Issue 2, 2001, Pages 326-335

  Merten, O W ^a   Cruz, P E ^a   Rochette, C ^a   Geny Fiamma, C ^a   Bouquet, C ^a   Goncalves D ^a   Danos, O ^a   Carrondo, M J T ^a  

^a GÉNÉTHON   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BIOTECHNOLOGICAL PROCEDURES; CELL CLONE; CELL DENSITY; CELL GROWTH; CELL LINE; CULTURE OPTIMIZATION; FIXED BED REACTOR; GIBBON LEUKEMIA VIRUS; LACZ GENE; PLAQUE FORMING UNIT; RETROVIRAL VECTOR; STIRRED REACTOR; SUSPENSION CELL CULTURE; VIRUS ENVELOPE PROTEIN; VIRUS GLYCOPROTEIN;

BIOREACTORS; CELL IMMOBILIZATION; CLONING; GLYCOLS; OPTIMIZATION; PROTEINS; VIRUSES;

GRADE VECTORS;

BIOTECHNOLOGY;

ALOCASIA MACRORRHIZOS; GIBBON APE LEUKEMIA VIRUS; HYLOBATES SP.; ILEX COOKII; UNIDENTIFIED RETROVIRUS;

EID: 0034923817     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp000162z     Document Type: Article

References (31)

1. An academic centre for gene therapy research with clinical grade manufacturing capability
2. Potential of gene therapy for Parkinson's disease: Neurobiologic issues and new developments in gene transfer methodologies
3. Biosafety monitoring of patients receiving intracerebral injections of murine retroviral vector producer cells
4. Virosomes: Cationic liposomes enhance retroviral transduction
5. Large-scale processing of recombinant retroviruses for gene therapy
6. Improved methods of retroviral vector transduction and production for gene therapy
7. Kinetics of retroviral production from the amphotropic ψCRIP murine producer cell line
8. Temperature significantly affects retroviral vector production and deactivation rates, and thereby determines retroviral titers
9. Kinetics of retrovirus production and decay
10. Fixed immobilized beds for the cultivation of animal cells
11. Fixed bed porous glass spheres (porosphere) bioreactors for animal cells
12. A fiber-bed bioreactor for anchorage dependent animal cell culture: Part 1. Bioreactor design and operations
13. Alternative surfaces for microcarrier culture of animal cells
14. A comparison of various laboratory scale culture configurations for microcarrier culture of animal cells
15. Thousand litre scale microcarrier culture of Vero cells for killed polio virus vaccine. Promising results
16. Oral polio vaccine (Sabin) produced on large-scale Vero cell culture
17. Animal cell bioreactor - Recent advances and challenges to scale-up
18. Hydrodynamic effects on animal cells grown in microcarrier cultures
19. Formation and disruption of animal cell aggregates in stirred vessels: Mechanisms and kinetic studies
20. Modeling retrovirus production for gene therapy. 1. Determination of optimal operation mode and harvest strategy
21. Functional characterization of adenoviral/retroviral chimeric vectors and their use for efficient screening of retroviral producer cell lines
22. New process for cell detachment: Use of heparin
23. Use of a recombinant retrovirus to study post-implantation cell lineage in mouse embryos
24. Evidence that monoclonal antibody production kinetics is related to the integral of viable cells curve in batch systems
25. Scale-up engineering in animal cell technology. Part II
26. Retrovirus expression during the cell cycle. I. Virus production, synthesis, and expression of viral proteins in Rauscher murine leukemia virus-infected mouse cells
27. Large scale production of retrovirus vectors
28. Antibody production in packed bed reactors using serum-free and protein-free medium
29. Justification of continuous packed-bed reactor for a retroviral vector production from amphotropic ψCRIP murine producer cell
30. Development of cell culture conditions for large scale retroviral vector production in reduced serum media
31. Development of a serum-free process for the efficient transduction of hematopoietic cells used in retroviral gene therapy against HIV