Mechanistic analysis of a multiple product sterol methyltransferase implicated in ergosterol biosynthesis in Trypanosoma brucei

Journal of Biological Chemistry

Volumn 281, Issue 10, 2006, Pages 6290-6296

  Zhou, Wenxu ^a   Lepesheva, Galina I ^b   Waterman, Michael R ^b   Nes, W David ^a  

^a TEXAS TECH UNIVERSITY   (United States)

^b VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOSYNTHESIS; CATALYSTS; DNA; DRUG PRODUCTS; ESCHERICHIA COLI; FUNGI; GEL PERMEATION CHROMATOGRAPHY; GENES; MICROORGANISMS; PLANTS (BOTANY); YEAST;

ERGOSTEROL; PHYTOSTEROLS; STEROL METHYLTRANSFERASE; TRYPANOSOMA BRUCEI; ZYMOSTEROL;

ENZYMES;

24,24 DIMETHYL ERGOSTA 8,25 (27) DIENOL; 24,25 EPIMINOLANOSTEROL; 25 AZALANOSTEROL; 26,27 DEHYDROZYMOSTEROL; ERGOSTA 8,24 (25) DIENOL; ERGOSTA 8,24 (28) DIENOL (FECOSTEROL); ERGOSTA 8,25 (27) DIENOL; ERGOSTEROL; GENOMIC DNA; METHYLTRANSFERASE; STEROL DERIVATIVE; STEROL METHYLTRANSFERASE; TETRAMER; UNCLASSIFIED DRUG; ZYMOSTEROL; ENZYME INHIBITOR; PROTOZOAL PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; CONTROLLED STUDY; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME SUBSTRATE; ESCHERICHIA COLI; GEL CHROMATOGRAPHY; GENE EXPRESSION; GENE ISOLATION; MASS SPECTROMETRY; MOLECULAR CLONING; NONHUMAN; PH MEASUREMENT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN PURIFICATION; PROTON NUCLEAR MAGNETIC RESONANCE; PROTOZOON; RECOMBINANT GENE; STEROL ANALYSIS; STEROL SYNTHESIS; TOPOGRAPHY; TRYPANOSOMA BRUCEI; AMINO ACID SEQUENCE; ANIMAL; BINDING SITE; BIOSYNTHESIS; CATALYSIS; CHEMISTRY; DRUG ANTAGONISM; ENZYME SPECIFICITY; ENZYMOLOGY; MASS FRAGMENTOGRAPHY; MOLECULAR GENETICS; PROTEIN MOTIF; SEQUENCE ALIGNMENT;

ESCHERICHIA COLI; FUNGI; PROTOZOA; TRYPANOSOMA BRUCEI;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; CATALYSIS; ENZYME INHIBITORS; ERGOSTEROL; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; METHYLTRANSFERASES; MOLECULAR SEQUENCE DATA; PROTOZOAN PROTEINS; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY; TRYPANOSOMA BRUCEI BRUCEI;

EID: 33646571616     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M511749200     Document Type: Article

References (36)

1. Dixon, H., Ginger, C. D., and Williamson, J. (1972) Comp. Biochem. Physiol. B 41, 1-18
2. Berriman, M. et al. (2005) Science 309, 416-422
3. Low, P., Dallner, G., Mayor, S., Cohen, S., Chait, B. T., and Menon, A. K. (1991) J. Biol. Chem. 266, 19250-19257
4. Lorente, S. O., Rodrigues, J. C. F., Jimenez, C. J., Joyce-Menekse, M., Rodrigues, C., Croft, S. L., Yardley, V., de Luca-Fradley, K., Ruiz-Perez, L. M., Urbina, J., de Souza, W., Pacanowska, D. G., and Gilbert, I. H. (2004) Antimicrob. Agents Chemother. 48, 2937-2950
5. Nes, W. D. (2000) Biochim. Biophys. Acta 1529, 63-88
6. Nes, W. D. (2005) Biochem. Soc. Trans. 33, 1189-1196
7. Roberts, C. W., McLeod, R., Rice, D. W., Ginger, M., Chance, M. L., and Goad, L. J. (2003) Mol. Biochem. Parasitol. 126, 129-142
8. Magaraci, F., Jimenez, C. J., Rodrigues, C., Rodrigues, J. C. F., Braga, V. M., Yardley, V., de Luca-Fradley, K., Croft, S. L., de Souza, W., Ruiz,-Perez, L. M., Urbina, J., Pacanowska, D. G., and Gilbert, I. H. (2003) J. Med. Chem. 46, 4714-4727
9. Zhou, W., Song, Z., Kanagasabai, R., Liu, J., Jayasimha, P., Sinha, A., Veeramachanemi, P., Miller, M. B., and Nes, W. D. (2003) Molecules 9, 185-203
10. Fonteneau, P., Hartmann, M. A., and Benveniste, P. (1977) Plant Sci. Lett. 10, 147-155
11. Rahier, A., Genot, J-C., Benveniste, P., and Narula, A. S. (1984) J. Biol. Chem. 259, 15213-15215
12. Guo, D., Jia, Z., and Nes, W. D. (1996) Tetrahedron Lett. 37, 6823-6826
13. Nes, W. D., Song, Z., Dennis, A. L., Zhou, W., Nam, J., and Miller, M. B. (2003) J. Biol. Chem. 278, 34505-34516
14. Nes, W. D., McCourt, B. S., Marshall, J. A., Ma, J., Dennis, A. L., Lopez, M., Li, H., and He, L. (1999) J. Org. Chem. 64, 1535-1542
15. Zhou, W., and Nes, W. D. (2003) Arch. Biochem. Biophys. 420, 18-34
16. Benveniste, P. (2004) Annu. Rev. Plant Biol. 55, 429-457
17. Pourshafie, M., Morand, S., Virion, A., Rakotmanga, M., Dupuy, C., and Loiseau, P. H. (2004) Antimicrob. Agents Chemother. 48, 2409-2414
18. Mangla, A. T., and Nes, W. D. (2000) Bioorg. Med. Chem. 8, 925-936
19. Kanagasabai, R., Zhou, W., Liu, J., Nguyen, T. T. M., Veeramachaneni, P., and Nes, W. D. (2004) Lipids 39, 737-746
20. Nes, W. D. (2003) Phytochemistry 64, 75-95
21. Bradford, M. M. (1976) Anal. Biochem. 72, 680-685
22. Nes, W. D., Jayasimha, P., Zhou, W., Kanagasabai, R., Jin, C., Jaradat, T. T., and Shaw, R. S. (2004) Biochemistry 43, 569-576
23. Nes, W. D., Janssen, G. G., and Bergenstrahle, A. (1991) J. Biol. Chem. 266, 15202-15212
24. Nes, W. D., Marshall, J. A., Jia, Z., Jaradat, T. T., Song, Z., and Jayasimha, P. (2002) J. Biol. Chem. 277, 42549-42556
25. Kagan, R. M., and Clarke, S. (1994) Arch. Biochem. Biophys. 310, 417-427
26. Fujimoto, Y., Sato, N., Iwai, K., Hamada, H., Yamada, J., and Morisaki, M. (1997) Chem. Commun. 681-682
27. Kalinowska, M., Nes, W. R., Crumley, F. G., and Nes, W. D. (1990) Phytochemistry 29, 3427-3434
28. Akihisa, T., Tamura, T., Matsumoto, T., Kokke, W. C. M. C., Ghosh, P., and Thakur, S. (1990) J. Chem. Soc. Perkin Trans. I, 2213-2218
29. Goad, L. J., Lenton, J. R., Knapp, F. F., and Goodwin, T. W. (1974) Lipids 9, 582-594
30. Arigoni, D. (1978) Ciba Found. Symp. 60, 243-258
31. Nes, W. D., McCourt, B. S., Zhou, W., Ma, J., Marshall, J. A., Peek, L-A., and Brennan, M. (1998) Arch. Biochem. Biophys. 353, 297-311
32. Nes, W. R., and Nes, W. D. (1980) Lipids in Evolution, pp. 157-197, Plenum, New York
33. Nes, W. D., Norton, R. A., Crumley, F. G., Madigan, S. J., and Katz, E. R. (1990) Proc. Natl. Acad. Sci. U. S. A. 87, 7565-7569
34. Lepesheva, G. I., Nes, W. D., Zhou, W., Hill, G. C., and Waterman, M. R. (2004) Biochemistry 43, 10789-10799
35. Hannaert, V., Saaverdra, E., Duffieux, F., Szikora, J-P., Rigden, D. J., Michels, P. A. M., and Opperdoes, F. R. (2002) Proc. Natl. Acad. Sci. U. S. A., 100, 1067-1071
36. Joubert, B. M., Buckner, F. S., and Matsuda, S. P. T. (2001) Org. Lett. 3, 1957-1960