Large-scale preparation of biologically active mouse and rat leptins and their L39A/D40A/F41A muteins which act as potent antagonists

Protein Expression and Purification

Volumn 47, Issue 1, 2006, Pages 128-136

  Salomon, Gili ^a,b   Niv Spector, Leonora ^a   Gussakovsky, Eugene E ^c   Gertler, Arieh ^a  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

^b Protein Forest Inc   (Israel)

^c UNIVERSITY OF MANITOBA   (Canada)

Author keywords

Leptin antagonists; Leptin site III; Mouse leptin; Rat leptin; Site directed mutagenesis

Indexed keywords

ALANINE; ASPARTIC ACID; ISOPROTEIN; LEPTIN; LEUCINE; PHENYLALANINE; RECOMBINANT PROTEIN;

AMINO ACID SUBSTITUTION; ANIMAL; ARTICLE; CELL LINE; DRUG ANTAGONISM; DRUG POTENTIATION; GENETICS; HUMAN; MOLECULAR CLONING; MOLECULAR GENETICS; MOUSE; NUCLEOTIDE SEQUENCE; RAT; SITE DIRECTED MUTAGENESIS; SYNTHESIS;

ALANINE; AMINO ACID SUBSTITUTION; ANIMALS; ASPARTIC ACID; BASE SEQUENCE; CELL LINE; CLONING, MOLECULAR; HUMANS; LEPTIN; LEUCINE; MICE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PHENYLALANINE; PROTEIN ISOFORMS; RATS; RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 33646107384     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.09.016     Document Type: Article

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