Preparation of recombinant bovine, porcine, and porcine W4R/R5K leptins and comparison of their activity and immunoreactivity with ovine, chicken, and human leptins

Protein Expression and Purification

Volumn 19, Issue 1, 2000, Pages 30-40

  Raver, Nina ^a   Gussakovsky, Eugene E ^b,c   Keisler, Duane H ^d   Krishna, Radha ^e   Mistry, Jehangir ^e   Gertler, Arieh ^a  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

^b BAR ILAN UNIVERSITY   (Israel)

^c VOLCANI CENTER   (Israel)

^d UNIVERSITY OF MISSOURI   (United States)

^e Diagnostic Systems Laboratories Inc   (United States)

Author keywords

Analogue; Bioactivity; Bovine; Cross immunoreactivity; Leptin; Porcine; Recombinant

Indexed keywords

BACTERIAL CELL; CATTLE; CELL PROLIFERATION; ESCHERICHIA COLI; GENETIC TRANSFORMATION; ION EXCHANGE CHROMATOGRAPHY; LEPTIN; MOLECULAR WEIGHT; NALIDIXIC ACID; PLASMID; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; RECOMBINANT PROTEIN; SDS POLYACRYLAMIDE GEL ELECTROPHORESIS; SHEEP; SWINE;

BACTERIA (MICROORGANISMS); BOS TAURUS; BOVINAE; CAPRA HIRCUS; ESCHERICHIA COLI; GALLUS GALLUS; ORYCTOLAGUS CUNICULUS; OVIS; OVIS ARIES; PROKARYOTA; SUIDAE; SUS SCROFA;

EID: 0034084568     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2000.1202     Document Type: Article

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