Gene expression in human hepatocytes in suspension after isolation is similar to the liver of origin, is not affected by hepatocyte cold storage and cryopreservation, but is strongly changed after hepatocyte plating

Drug Metabolism and Disposition

Volumn 34, Issue 5, 2006, Pages 870-879

  Richert, Lysiane ^a,b,f   Liguori, Michael J ^c   Abadie, Catherine ^a,b   Heyd, Bruno ^d   Mantion, Georges ^d   Halkic, Nermin ^e   Waring, Jeffrey F ^c  

^a Unité de Formation et de Recherche des Sciences Médicales et Pharmaceutiques   (France)

^b KaLy Cell   (France)

^c ABBOTT LABORATORIES   (United States)

^d UNIVERSITY HOSPITAL JEAN MINJOZ   (France)

^e LAUSANNE UNIVERSITY HOSPITAL   (Switzerland)

^f UNIVERSITÉ DE FRANCHE COMTÉ   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ADULT; APOPTOSIS; ARTICLE; CELL CULTURE; CELL ISOLATION; CELL METABOLISM; CELL SUSPENSION; CONTROLLED STUDY; CRYOPRESERVATION; FATTY ACID METABOLISM; FEMALE; GENE EXPRESSION; HUMAN; HUMAN CELL; HUMAN TISSUE; LIPID METABOLISM; LIVER; LIVER CELL; LIVER METABOLISM; OXIDATIVE STRESS; PLATING MEDIUM; PRIORITY JOURNAL; PROTEIN PROCESSING; RECYCLING; THAWING;

CELL SEPARATION; CELLS, CULTURED; CRYOPRESERVATION; CYTOLOGICAL TECHNIQUES; GENE EXPRESSION; HEPATOCYTES; HUMANS; LIVER; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; RNA; TISSUE PRESERVATION;

EID: 33645821905     PISSN: 00909556     EISSN: 1521009X     Source Type: Journal    
DOI: 10.1124/dmd.105.007708     Document Type: Article

References (40)

1. Alexandre E, Viollon-Abadie C, David P, Coassolo P, Heyd B, Mantion G, Wolf P, Bachellier P, Jaeck D, and Richert L (2002) Cryopreservation of adult human hepatocytes obtained from resected liver biopsies. Cryobiology 44:103-113.
2. Babenko NA, Ruiz-Larrea MB, Martinez R, Martin C, and Lacort M (1998) Inhibition by estrogens of the oxidant-mediated mobilization of arachidonic acid in hepatocytes. J Physiol Biochem 54:77-84.
3. Binda D, Lasserre-Bigot D, Bonet A, Thomassin M, Come MP, Guinchard C, Bars R, Jacqueson A, and Richert L (2003) Time course of cytochromes P450 decline during rat hepatocyte isolation and culture: effect of L-NAME. Toxicol In Vitro 17:59-67.
4. Blanchard N, Alexandre E, Abadie C, Lave T, Heyd B, Mantion G, Jaeck D, Richert L, and Coassolo P (2005) Comparison of clearance predictions using primary cultures and suspensions of human hepatocytes. Xenobiotica 35:1-15.
5. Chandra P and Brouwer KL (2004) The complexities of hepatic drug transport: current knowledge and emerging concepts. Pharm Res 21:719-735.
6. Dahlquist K, Salomonis N, Vranizan K, Lawlor SC, and Conklin BR (2002) GenMAPP, a new tool for viewing and analyzing microarray data on biological pathways. Nat Genet 31:19-20.
7. Donato MT, Serralta A, Jimenez N, Perez G, Castell JV, Mir J, and Gomez-Lechon MJ (2005) Liver grafts preserved in Celsior solution as source of hepatocytes for drug metabolism studies: comparison with surgical liver biopsies. Drug Metab Dispos 33:108-114.
8. Elkon H, Melamed E, and Offen D (2004) Oxidative stress, induced by 6-hydroxydopamine, reduces proteasome activities in PC12 cells: implications for the pathogenesis of Parkinson's disease. J Mol Neurosci 24:387-400.
9. Fang CH, Li BG, Fischer DR, Wang JJ, Runnels HA, Monaco JJ, and Hasselgren PO (2000) Burn injury upregulates the activity and gene expression of the 20 S proteasome in rat skeletal muscle. Clin Sci (Lond) 99:181-187.
10. Garcia M, Rager J, Wang Q, Strab R, Hidalgo IJ, Owen A, and Li J (2003) Cryopreserved human hepatocytes as alternative in vitro model for cytochrome p450 induction studies. In Vitro Cell Dev Biol Anim 39:283-287.
11. Gomez-Lechon MJ, Donato MT, Castell JV, and Jover R (2004) Human hepatocytes in primary culture: the choice to investigate drug metabolism in man. Curr Drug Metab 5:443-462.
12. Gu S, Roderick HL, Camacho P, and Jiang JX (2001) Characterization of an N-system amino acid transporter expressed in retina and its involvement in glutamine transport. J Biol Chem 276:24137-24144.
13. Hamilton GA, Jolley SL, Gilbert D, Coon DJ, Barros S, and LeCluyse EL (2001) Regulation of cell morphology and cytochrome P450 expression in human hepatocytes by extracellular matrix and cell-cell interactions. Cell Tissue Res 306:85-99.
14. Hobler SC, Williams A, Fischer D, Wang JJ, Sun X, Fischer JE, Monaco JJ, and Hasselgren PO (1999) Activity and expression of the 20S proteasome are increased in skeletal muscle during sepsis. Am J Physiol 277:R434-R440.
15. Ishihara Y, Shiba D, and Shimamoto N (2005) Primary hepatocyte apoptosis is unlikely to relate to caspase-3 activity under sustained endogenous oxidative stress. Free Radic Res 39:163-173.
16. Jigorel E, Le Vee M, Boursier-Neyret C, Bertrand M, and Fardel O (2005) Functional expression of sinusoidal drug transporters in primary human and rat hepatocytes. Drug Metab Dispos 33:1418-1422.
17. Jump DB (2002) Dietary polyunsaturated fatty acids and regulation of gene transcription. Curr Opin Lipidol 13:155-164.
18. Lave T, Coassolo P, and Reigner B (1999) Prediction of hepatic metabolic clearance based on interspecies allometric scaling techniques and in vitro-in vivo correlations. Clin Pharmacokinet 36:211-231.
19. LeCluyse EL, Alexandre E, Hamilton GA, Viollon-Abadie C, Coon DJ, Jolley S, and Richert L (2005) Isolation and culture of primary human hepatocytes. Methods Mol Biol 290:207-229.
20. Li AP (2001) Screening for human ADME/Tox drug properties in drug discovery. Drug Discov Today 6:357-366.
21. Li YQ, Prentice DA, Howard ML, Mashford ML, and Desmond PV (1999) The effect of hormones on the expression of five isoforms of UDP- glucuronosyltransferase in primary cultures of rat hepatocytes. Pharm Res 16:191-197.
22. Liguori MJ, Anderson MG, Bukofzer S, McKim J, Pregenzer JF, Retief J, Spear BB, and Waring JF (2005) Microarray analysis in human hepatocytes suggests a mechanism for hepatotoxicity induced by trovafloxacin. Hepatology 41:177-186.
23. Liu G, Loraine AE, Shigeta R, Cline M, Cheng J, Valmeekam V, Sun S, Kulp D, and Siani-Rose MA (2003) NetAffx: Affymetrix probesets and annotations. Nucleic Acids Res 31:82-86.
24. Luttringer O, Theil FP, Lave T, Wernli-Kuratli K, Guentert TW, and de Saizieu A (2002) Influence of isolation procedure, extracellular matrix and dexamethasone on the regulation of membrane transporters gene expression in rat hepatocytes. Biochem Pharmacol 64:1637-1650.
25. Madan A, Graham RA, Carroll KM, Mudra DR, Burton LA, Krueger LA, Downey AD, Czerwinski M, Forster J, Ribadeneira MD, et al. (2003) Effects of prototypical microsomal enzyme inducers on cytochrome P450 expression in cultured human hepatocytes. Drug Metab Dispos 31:421-431.
26. Meiners S, Heyken D, Weller A, Ludwig A, Stangl K, Kloetzel PM, and Kruger E (2003) Inhibition of proteasome activity induces concerted expression of proteasome genes and de novo formation of mammalian proteasomes. J Biol Chem 278:21517-21525.
27. O'Brien PJ, Chan K, and Silber PM (2004) Human and animal hepatocytes in vitro with extrapolation in vivo. Chem Biol Interact 150:97-114.
28. Pan M, Cederbaum AI, Zhang YL, Ginsberg HN, Williams KJ, and Fisher EA (2004) Lipid peroxidation and oxidant stress regulate hepatic apolipoprotein B degradation and VLDL production. J Clin Investig 113:1277-1287.
29. Rauen U, Polzar B, Stephan H, Mannherz HG, and de Groot H (1999) Cold-induced apoptosis in cultured hepatocytes and liver endothelial cells: mediation by reactive oxygen species. FASEB J 13:155-168.
30. Richert L, Alexandre E, Lloyd T, Orr S, Viollon-Abadie C, Patel R, Kingston S, Berry D, Dennison A, Heyd B, et al. (2004) Tissue collection, transport and isolation procedures required to optimise human hepatocyte isolation from waste liver surgical resections. A multi-laboratory study. Liver Int 24:371-378.
31. Richert L, Binda D, Hamilton G, Viollon-Abadie C, Alexandre E, Bigot-Lasserre D, Bars R, Coassolo P, and LeCluyse E (2002) Evaluation of the effect of culture configuration on morphology, survival time, antioxidant status and metabolic capacities of cultured rat hepatocytes. Toxicol In Vitro 16:89-99.
32. Richert L, Lamboley C, Viollon-Abadie C, Grass P, Hartmann N, Laurent S, Heyd B, Mantion G, Chibout SD, and Staedtler F (2003) Effects of clofibric acid on mRNA expression profiles in primary cultures of rat, mouse and human hepatocytes. Toxicol Appl Pharmacol 191:130-146.
33. Rippin SJ, Hagenbuch B, Meier PJ, and Stieger B (2001) Cholestatic expression pattern of sinusoidal and canalicular organic anion transport systems in primary cultured rat hepatocytes. Hepatology 4:776-782.
34. Roymans D, Annaert P, Van Houdt J, Weygers A, Noukens J, Sensenhauser C, Silva J, Van Looveren C, Hendrickx J, Mannens G, et al. (2005) Expression and induction potential of cytochromes P450 in human cryopreserved hepatocytes. Drug Metab Dispos 33:1004-1016.
35. Shitara Y, Li AP, Kato Y, Lu C, Ito K, Itoh T, and Sugiyama Y (2003) Function of uptake transporters for taurocholate and estradiol 17β-D-glucuronide in cryopreserved human hepatocytes. Drug Metab Pharmacokinet 18:33-41.
36. Swagell CD, Henly DC, and Morris CP (2005) Expression analysis of a human hepatic cell line in response to palmitate. Biochem Biophys Res Commun 328:432-441.
37. Wang H and LeCluyse EL (2003) Role of orphan nuclear receptors in the regulation of drug-metabolising enzymes. Clin Pharmacokinet 42:1331-1357.
38. Waring JF, Ciurlionis R, Jolly RA, Heindel M, Gagne G, Fagerland JA, and Ulrich RG (2003) Isolated human hepatocytes in culture display markedly different gene expression patterns depending on attachment status. Toxicol In Vitro 17:693-701.
39. Waring JF, Jolly RA, Ciurlionis R, Lum PY, Praestgaard JT, Morfitt DC, Buratto B, Roberts C, Schadt E, and Ulrich RG (2001) Clustering of hepatotoxins based on mechanism of toxicity using gene expression profiles. Toxicol Appl Pharmacol 175:28-42.
40. Zurlo J and Arterburn LM (1996) Characterization of a primary hepatocyte culture system for toxicological studies. In Vitro Cell Dev Biol Anim 32:211-220.