Cryopreserved human hepatocytes as alternative in vitro model for cytochrome P450 induction studies

In Vitro Cellular and Developmental Biology - Animal

Volumn 39, Issue 7, 2003, Pages 283-287

  Garcia, Martha ^a   Rager, Joseph ^a   Wang, Qing ^a   Strab, Robert ^a   Hidalgo, Ismael J ^a   Owen, Albert ^a   Li, Jibin ^a  

^a Oaklands Corporate Center   (United States)

Author keywords

CYP1A1 2; CYP3A4; Drug drug interactions; Drug screening model

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; BETA NAPHTHOFLAVONE; CYTOCHROME P450; CYTOCHROME P450 1A1; CYTOCHROME P450 1A2; CYTOCHROME P450 3A4; ETHOXYRESORUFIN DEETHYLASE; MESSENGER RNA; OXIDOREDUCTASE; RIFAMPICIN; XENOBIOTIC AGENT;

ADULT; AGED; ARTICLE; CELL ADHESION; CELL STRUCTURE; CELL SUSPENSION; CELL VIABILITY; CONTROLLED STUDY; CRYOPRESERVATION; CULTURE MEDIUM; DONOR; DRUG SCREENING; ENZYME ACTIVITY; ENZYME INDUCTION; FEMALE; GENE EXPRESSION; HUMAN; HUMAN CELL; IN VITRO STUDY; LIVER CELL; MALE; MODEL; REDUCTION;

ADULT; AGED; ANIMALS; CELL SIZE; CELL SURVIVAL; CELLS, CULTURED; CRYOPRESERVATION; CULTURE MEDIA, SERUM-FREE; CYTOCHROME P-450 CYP1A1; CYTOCHROME P-450 CYP1A2; CYTOCHROME P-450 ENZYME SYSTEM; ENZYME ACTIVATION; ENZYME INHIBITORS; FEMALE; GENE EXPRESSION REGULATION; HEPATOCYTES; HUMANS; HYDROXYTESTOSTERONES; MALE; MIDDLE AGED; MODELS, BIOLOGICAL; OXAZINES; RIFAMPIN; RNA, MESSENGER;

EID: 1042265446     PISSN: 10712690     EISSN: None     Source Type: Journal    
DOI: 10.1290/1543-706X(2003)039<0283:CHHAAI>2.0.CO;2     Document Type: Article

References (18)

1. Bapiro, T.; Anderson, T. B.; Otter, C.; Hasler, J. A.; Masimirembwa, C. M. Cytochrome P450 1A1/2 induction by antiparasitic drugs: dose-dependent increase in ethoxyresorufin O-deethylase activity and mRNA caused by quinine, primaquine and albendazole in HepG2 cells. Eur. J. Clin. Pharmacol. 58:537-542; 2002.
2. Bjornsson, T. D.; Callaghan, J. T.; Einolf, H. J., et al. The conduct of in vitro and in vivo drug-drug interaction studies: a pharmaceutical research and manufacturers of America (PhRMA) perspective. Drug Metab. Dispos. 31:815-832; 2003.
3. Chr.'t Hoen, P. A.; Commandeur, J. N. M.; Vermeulen, N. P. E.; Van Berkel, T. J. C.; Bijsterbosch, M. K. Selective induction of cytochrome P450 3A1 by dexamethasone in cultured rat hepatocytes. Biochem. Pharmacol. 60:1509-1518; 2000.
4. de Sousa, G.; Langouët, S.; Nicolas, F.; Lorenzon, G.; Placidi, M.; Rahmani, R.; Guillouzo, A. Increase of cytochrome P-4501A and glutathione transferase transcripts in cultured hepatocytes from dogs, monkeys. and humans after cryopreservation. Cell Biol. Toxicol. 12:351-358; 1996.
5. Donato, M. T.; Castell, J. V. Strategies and molecular probes to investigate the role of cytochrome P450 in drug metabolism. Clin. Pharmacokinet. 42:154-178; 2003.
6. Donato, M. T.; Gómez-Lechón, M. J.; Castell, J. V. A microassay for measuring cytochrome P4501A1 and P450IIB1 activities in intact human and rat hepatocytes cultured on 96-well plates. Anal. Biochem. 213:29-33; 1993.
7. Gómez-Lechón, M. J.; Donato, T.; Jover, R.; Rodriguez, C.; Ponsoda, X.; Glaise, D.; Castell, J. V.; Guguen-Guillouzo, C. Expression and induction of a large set of drug-metabolizing enzymes by the highly differentiated human hepatoma cell line BC2. Eur. J. Biochem. 268:1448-1459; 2001.
8. Hengstler, J. G.; Utesch, D.; Steinberg, P., et al. Cryopreserved primary hepatocytes as a constantly available in vitro model for the evaluation of human and animal drug metabolism and enzyme induction. Drug Metab. Rev. 32:81-118; 2000.
9. Hewitt, N. J.; Büring, K.-U.; Dasenbrock, J.; Haunschild, J.; Ladstetter, B.; Utesch, D. Studies comparing in vivo: in vitro metabolism of three pharmaceutical compounds in rat, dog, monkey and human using cryopreserved hepatocytes, microsomes and collagen gel immobilized hepatocyte cultures. Drug Metab. Dispos. 29:1042-1050; 2001.
10. Li, A. P.; Lu, C.; Brent, J. A.; Pham. C.; Fackett, A.; Ruegg, C. E.; Silber, P. M. Cryopreserved human hepatocytes: characterization of drug-metabolizing enzyme activities and applications in higher throughput screening assays for hepatotoxicity, metabolic stability, and drug-drug interaction potential. Chem. Biol. Interact. 121:17-35: 1999.
11. Madan, A.; Dehaan, R.; Mudra, D.; Carroll, K.; LeCluyse, E.; Parkinson, A. Effect of cryopreservation on cytochrome P-450 enzyme indnction in cultured rat hepatocytes. Drug Metab. Dispos. 27:327-335; 1999.
12. Madan, A.; Graham, R. A.; Carroll, K. M., et al. Effects of prototypical microsomal enzyme inducers on cytochrome P450 expression in cultured human hepatocytes. Drug Metab. Dispos. 31:421-431: 2003.
13. Marshall, N. J.; Goodwin, C. J.; Holt, S. J. A critical assessment of the use of microculture tetrazolium assays to measure cell growth and function. Growth Regul. 5:69-84; 1995.
14. Raucy, J. L. Regulation of CYP3A4 expression in human hepatocytes by pharmaceuticals and natural products. Drug Metab, Dispos. 31:533-539; 2003.
15. Rodriguez-Antona, C.; Donato, M. T.; Boobis, A.; Edwards, R. J.; Watts, P. S.; Castell, J. V.; Gómez-Lechón, M.-J. Cytochrome P450 expression in human hepatocytes and hepatoma cell lines: molecular mechanisms that determine lower expression in cultured cells. Xenobiotica 32:505-520; 2002.
16. Shibata, Y.; Takahashi, H.; Chiba, M.; Ishii, Y. Prediction of hepatic clearance and availability by cryopreserved human hepatocytes: an application of serum incubation method. Drug Metab. Dispos. 30:892-896; 2002.
17. Silva, J. M.; Day, S. H.; Nicoll-Griffith, D. A. Induction of cytochrome-P450 in cryopreserved rat and human hepatocytes. Chem. Biol. Interact. 121:49-63; 1999.
18. Silva, J. M.; Morin, P. E.; Day, S. H.; Kennedy, B. P.; Payette, P.; Rushmore, T.; Yerger, J. A.; Nicoll-Griffith, D. A. Refinement of an in vitro cell model for cytochrome P450 induction. Drug Metab. Dispos. 26:490-496; 1998.