Evaluation of the effect of culture configuration on morphology, survival time, antioxidant status and metabolic capacities of cultured rat hepatocytes

Toxicology in Vitro

Volumn 16, Issue 1, 2002, Pages 89-99

  Richert, L ^a   Binda, D ^a   Hamilton, G ^b   Viollon Abadie, C ^a   Alexandre, E ^c   Bigot Lasserre, D ^d   Bars, R ^d   Coassolo, P ^e   LeCluyse, E ^b  

^a UNIVERSITÉ DE FRANCHE COMTÉ   (France)

^b UNIVERSITY OF NORTH CAROLINA   (United States)

^c Fondation Transplantation   (France)

^d BAYER CROPSCIENCE AG   (Germany)

^e F HOFFMANN LA ROCHE LTD   (Switzerland)

Author keywords

Antioxidant status; Culture matrices; Metabolic capacities; Rat hepatocytes after isolation and in culture

Indexed keywords

ANTIOXIDANT; CATALASE; GLUCURONOSYLTRANSFERASE; GLUTATHIONE; GLUTATHIONE PEROXIDASE; GLUTATHIONE REDUCTASE; GLUTATHIONE TRANSFERASE; THIOBARBITURIC ACID REACTIVE SUBSTANCE; UNSPECIFIC MONOOXYGENASE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL ADHESION; CELL CULTURE; CELL ISOLATION; CELL STRUCTURE; CELL SURVIVAL; CONTROLLED STUDY; DETOXIFICATION; ENZYME ACTIVITY; LIPID PEROXIDATION; LIVER CELL; LONGEVITY; MALE; METABOLIC CAPACITY; NONHUMAN; PROTEIN INDUCTION; RAT;

ANIMALS; ANTIOXIDANTS; CELL CULTURE TECHNIQUES; CELLS, CULTURED; ENZYMES; GLUTATHIONE; HEPATOCYTES; LIPID PEROXIDATION; MALE; MICROSOMES, LIVER; RATS; RATS, SPRAGUE-DAWLEY; TIME FACTORS;

EID: 0036153552     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0887-2333(01)00099-6     Document Type: Article

References (56)

1. Differential regulation of glutathione S-transferases in cultured hepatocytes
2. Morphological comparison and functional reconstitution of rat hepatic parenchymal cells on various matrices
3. Effect of extracellular matrix composition of the expression of glutathione S-transferase isoenzymes in organotypical hepatocyte cultures
4. Isolated hepatocytes preparation, properties and applications
5. Hepatic antioxidant status in normotensive (WKY, SPRD) and hypertensive (SHR, DOCA) rats. Ex vivo and in vitro data
6. Characterization of a pretranscriptional pathway for induction by phenobarbital of cytochrome P450 3A23 in primary cultures of adult rat hepatocytes
7. Metabolic capacities in cultured human hepatocytes obtained by a new isolating procedure from non-wedge small liver biopsies
8. Effects of extracellular matrix on the expression of peroxisomes in primary rat hepatocyte cultures
9. Collagen type 1 gel cultures of adult rat hepatocytes as a screening induction model for cytochrome P450-dependent enzymes
10. Co-cultures of hepatocytes with epithelial-like cell lines: Expression of drug-biotransformation activities by hepatocytes
11. Long-term in vitro function of adult hepatocytes in a collagen sandwich configuration
12. New tables for multiple comparisons with a control
13. Regulation of hepatic nitric oxide synthase by reactive oxygen intermediates and glutathione
14. Biochemical mechanisms
15. Induction of monooxygenase and UDP-glucuronosyltransferase activities in primary cultures of rat hepatocytes
16. The activity of UDP-glucuronosyltransferase, sulfotransferase and glutathione-S-transferase in primary culture of rat hepatocytes
17. Comparison of the effects of various peroxisome proliferators on peroxisomal enzyme activities, DNA synthesis and apoptosis in rat and human hepatocyte cultures
18. An in vitro model for peroxisome proliferation utilizing primary hepatocytes in sandwich culture
19. Effect of extracellular matrix on the expression of peroxisome proliferation associated genes in cultured rat hepatocytes
20. Evaluation of the effect of culture matrices on induction of CYP3A isoforma in cultured porcine hepatocytes
21. The protective role of glutathione peroxidase in apoptosis induced by reactive oxygen species
22. Expression of multiple forms of P450s mRNAs in primary cultures of rat hepatocytes maintained on Matrigel
23. Regulation of glutathione S-transferase enzymes in primary cultures of rat hepatocytes maintained under various matrix configurations
24. Influence of extracellular matrix overlay and medium formulation on the induction of cytochrome P-450 2B enzymes in primary cultures of rat hepatocytes
25. Strategies for restoration and maintenance of normal hepatic structure and function in long-term cultures of rat hepatocytes
26. Cultured rat hepatocytes
27. The effect of hormones on the expression of five isoforms of UDP-glucuronosyltransferase in primary cultures of rat hepatocytes
28. Endogenous nitric oxide is responsible for the early loss of P-450 in cultured rat hepatocytes
29. Peroxynitrite generated from constitutive nitric oxide synthase mediates the early biochemical injury in short-term cultured hepatocytes
30. Effect of cryopreservation on cytochrome P450 enzyme induction in cultured rat hepatocytes
31. Glutathione dependent detoxication in adult rat hepatocytes under various culture conditions
32. Historical review of hepatocyte transplantation: The road from whole liver transplantation to hepatocyte transplantation
33. Cell-cell interactions are essential for maintenance of hepatocyte function in collagen gel but not on Matrigel
34. Glutathione status in primary cultures of rat hepatocytes and its role in cell attachment to collagen
35. Rat adult hepatocytes in primary pure and mixed monolayer culture: Comparison of the maintenance of mixed function oxidase and conjugation pathways of drug metabolism
36. Effects of freezing, thawing, and storing human liver microsomes on cytochrome P450 activity
37. Cell-substratum adhesion strength as a determinant of hepatocyte aggregate morphology
38. The effects of cell density, attachment substratum and dexamethasone on spontaneous apoptosis of rat hepatocytes in primary culture
39. Preparation of isolated rat liver hepatocytes
40. Rapid induction of NF-kB binding during liver cell isolation and culture: Inhibition by L-NAME indicates a role for nitric oxide synthase
41. Development of a bioartificial liver: Properties and function of a hollow-fiber module inoculated with liver cells
42. Effects of dimethyl sulfoxide on phase I and phase II biotransformation in cultured rat hepatocytes
43. Mechanochemical manipulation of hepatocyte aggregation can selectively induce or repress liver-specific function
44. Attenuation of endogenous oxidative stress-induced cell death by cytochrome P450 inhibitors in primary cultures of rat hepatocytes
45. Refinement of an in vitro cell model for cytochrome P450 induction
46. Problems in using isolated and cultured hepatocytes for xenobiotic metabolism-based toxicity testing - Solutions?
47. Regulation of testosterone hydroxylation by rat liver microsomal cytochrome P-450
48. Effect of N-dicyclopropylmethyl-amino-2-oxazoline (S-3341) on antioxidant status and nitric oxide in hypertensive patients
49. Inhibition of cytochromes P450A by nitric oxide
50. Characterization of nitric oxide production following isolation of rat hepatocytes
51. Inhibition of cytochrome P450 by nitric oxide and a nitric oxide-releasing agent
52. Phenobarbital, β-Naphtoflavone, Clofibrate and Pregnenolone-16α-Carbonitrile do not affect hepatic thyroid hormone UDP-glucuronosyl transferase activity and thyroid function in mice
53. Effects of model inducers on thyroxine UDP-glucuronosyl transferase activity in vitro in rat and mouse hepatocyte cultures
54. Post-isolation inducible nitric oxide synthase gene expression due to collagenase buffer perfusion and characterization of the gene regulation in primary cultured murine hepatocytes
55. Phenobarbital induction of cytochromes P450: High-level long-term responsiveness of primary rat hepatocyte cultures to drug induction, and glucocorticoid dependence of the phenobarbital response
56. The isoenzyme pattern of cytochrome P450 in rat hepatocytes in primary culture, comparing different enzyme activities in microsomal incubations and in intact monolayers