Green fluorescent protein applications in Drosophila

Methods of Biochemical Analysis

Volumn 47, Issue , 2005, Pages 227-257

  Hazelrigg, Tulle ^a   Mansfield, Jennifer H ^a,b  

^a Och Spine at New York Presbyterian Hospitals   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN;

ANIMAL; ARTICLE; CYTOLOGY; DROSOPHILA; GENE VECTOR; GENETICS; HYDROZOA; MEIOSIS; MITOSIS; NERVE CELL; PHYSIOLOGY; REPORTER GENE; SENSITIVITY AND SPECIFICITY;

ANIMALS; DROSOPHILA; GENES, REPORTER; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HYDROZOA; MEIOSIS; MITOSIS; NEURONS; RECOMBINANT FUSION PROTEINS; SENSITIVITY AND SPECIFICITY;

CYTOLOGY; MOLECULAR BIOLOGY; PHOTOBLEACHING;

FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING; GREEN FLUORESCENT PROTEIN;

FLUORESCENCE;

EID: 33644628212     PISSN: 00766941     EISSN: None     Source Type: Book Series    
DOI: 10.1002/0471739499.ch11     Document Type: Article

References (97)

1. Ahmad, K., and Henikoff, S. (2001). Modulation of a transcription factor counteracts heterochromatic gene silencing in Drosophila. Cell 104:839-847.
2. Aubin, J. E. (1979). Autofluorescence of viable cultured mammalian cells. J. Histochem. Cytochem. 27:36043.
3. Barolo, S., Carver, L. A., and Posakony, J. W. (2000). GFP and beta-galactosidase transformation vectors for promoter/enhancer analysis in Drosophila. Biotechniques 29:726-732.
4. Barthmaier, P., and Fyrberg, E. (1995). Monitoring development and pathology of Drosophila indirect flight muscles using green fluorescent protein. Dev. Biol. 169:770-774.
5. Berdnik, D., and Knoblich, J. A. (2002). Drosophila Aurora-A is required for centrosome maturation and actin-dependent asymmetric protein localization during mitosis. Curr. Biol. 12:640-647.
6. Bertrand, E., Chartrand, P., Schaefer, M., Shenoy, S. M., Singer, R. H., and Long, R. M. (1998). Localization of ASH1 mRNA particles in living yeast. Mol. Cell 2:437-445.
7. Bhalero, S., Sen, A., Stocker, R., and Rodrigues, V. (2003). Olfactory neurons expressing identified receptor genes project to subsets of glomeruli within the antennal lobe of Drosophila melanogaster. J. Neurobiol. 54:577-592.
8. Bharathi, V., Pallavi, S. K., Bajpai, R., Emerald, B. S., and Shashidhara, L. S. (2004). Genetic characterization of the Drosophila homologue of coronin. J. Cell Sci. 117:1911-1922.
9. Bobinnec, Y., Marcaillou, C., Morin, X., and Debec, A. (2003). Dynamics of the endoplasmic reticulum during early development of Drosophila melanogaster. Cell Motil. Cytoskeleton 54:217-225.
10. Bossing, T., Barros, C. S., and Brand, A. H. (2002). Rapid tissue-specific expression assay in living embryos. Genesis 34:123-126.
11. Brand, A. H., and Perrimon, N. (1993). Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development 118:401-415.
12. Brand, A. H. (1995). GFP in Drosophila. Trends Genet. 11:324-325.
13. Brennecke, J., Hipfner, D. R., Stark, A., Russell, R. B., and Cohen, S. M. (2003). bantam encodes a developmentally regulated microRNA that controls cell proliferation and regulates the proapoptotic gene hid in Drosophila. Cell 113:25-36.
14. Brown, S., and Castelli-Gair Hombria, J. (2000). Drosophila grain encodes a GATA transcription factor required for cell rearrangement during morphogenesis. Development 127:4867-4876.
15. Brumby, A. M., and Richardson, H. E. (2003). scribble mutants cooperate with oncogenic Ras or Notch to cause neoplastic overgrowth in Drosophila. EMBO J. 22:5769-5779.
16. Casso, D., Ramirez-Weber, F., and Kornberg, T. B. (2000). GFP-tagged balancer chromosomes for Drosophila melanogaster. Mech. Dey. 91:451-454.
17. Cha, B. J., Koppetsch, B. S., and Theurkauf, W. E. (2001). In vivo analysis of Drosophila bicoid mRNA localization reveals a novel microtubule-dependent axis specification pathway. Cell 106:35-46.
18. Chalfie, M., Tu, Y., Euskirchen, G., Ward, W. W., and Prasher, D. C. (1994). Green fluorescent protein as a marker for gene expression. Science 263:802-805.
19. Clarkson, M., and Saint, R. (1999). A His2AvDGFP fusion gene complements a lethal His2AvD mutant allele and provides an in vivo marker for Drosophila chromosome behavior. DNA Cell Biol. 18:457-462.
20. Clyne, P. J., Brotman, J. S., Sweeney, S. T., and Davis, G. (2003). Green fluorescent protein tagging Drosophila proteins at their native genomic loci with small P elements. Genetics 165:1433-1441.
21. Cox, R. T., and Spradling, A. C. (2003). A Balbiani body and the fusome mediate mitochondrial inheritance during Drosophila oogenesis. Development 130:1579-1590.
22. Datar, S. A., Jacobs, H. W., de la Cruz, A. F., Lehner, C. F., and Edgar, B. A. (2000). The Drosophila cyclin D-Cdk4 complex promotes cellular growth. EMBO J. 19:4543-4554.
23. Davis, I., Girdham, C. H., and O'Farrell, P. H. (1995). A nuclear GFP that marks nuclei in living Drosophila embryos; maternal supply overcomes a delay in the appearance of zygotic fluorescence. Dev. Biol. 170:726-729.
24. Dietzel, S., Niemann, H., Bruckner, B., Maurange, C., and Paro, R. (1999). The nuclear distribution of Polycomb during Drosophila melanogaster development shown with a GFP fusion protein. Chromosoma 108:83-94.
25. Dorman, J. B., James, K. E., Fraser, S. E., Kiehart, D. P., and Berg, C. A. (2004). Bullwinkle is required for epithelial morphogenesis during Drosophila oogenesis. Dev. Biol. 267:320-341.
26. Duffy, J. B. (2002). GAL4 system in Drosophila: A fly geneticist's Swiss army knife. Genesis 34(1-2):1-15.
27. Dutta, D., Bloor, J. W., Ruiz-Gomez, M., Vijay Raghavan, K., and Kiehart, D. P. (2002). Real-time imaging of morphogenetic movements in Drosophila using Gal4-UAS-driven expression of GFP fused to the actin-binding domain of moesin. Genesis 34:146-151.
28. Edwards, K. A., Demsky, M., Montague, R. A., Weymouth, N., and Kiehart, D. P. (1997). GFP-Moesin illuminates actin cytoskeleton dynamics in living tissue and demonstrates cell shape changes during morphogenesis in Drosophila. Dev. Biol. 191:103-117.
29. Endow, S., and Komma, D. (1996). Centrosome and spindle function of the Drosophila Ncd microtubule motor visualized in live embryos using Ncd-GFP fusion proteins. J. Cell Sci. 109:2429-2442.
30. Endow, S., and Komma, D. (1997). Spindle dynamics during meiosis in Drosophila oocytes. J. Cell Biol. 137:1321-1336.
31. Entchev, E. V., Schwabedissen, A., and Gonzalez-Gaitan, M. (2000). Gradient formation of the TGF-beta homolog Dpp. Cell 103:981-991.
32. Ferrandon, D., Jung, A. C., Criqui, M., Lemaitre, B., Uttenweiler-Joseph, S., Michaut, L., Reichhart, J., and Hoffmann, J. A. (1998). A drosomycin-GFP reporter transgene reveals a local immune response in Drosophila that is not dependent on the Toll pathway. EMBO J. 17:1217-1227.
33. Forrest, K. M., and Gavis, E. R. (2003). Live imaging of endogenous RNA reveals a diffusion and entrapment mechanism for nanos mRNA localization in Drosophila. Curr. Biol. 13:1159-1168.
34. Furlong, E. E., Profitt, D., and Scott, M. P. (2001a). Automated sorting of live transgenic embryos. Nat. Biotechnol. 19:153-156.
35. Furlong, E. E., Andersen, E. C., Null, B., White, K. P., and Scott, M. P. (2001b). Patterns of gene expression during Drosophila mesoderm development. Science 293:1629-1633.
36. Gerlitz, O., Nellen, D., Ottiger, M., and Basler, K. (2002). A screen for genes expressed in Drosophila imaginal discs. Int. J. Dev. Biol. 46:173-176.
37. Gibson, M. C., Lehman, D. A., and Schubiger, G. (2002). Lumenal transmission of Decapentaplegic in Drosophila imaginal discs. Dev. Cell 3:451-460.
38. Grieder, N. C., de Cuevas, M., and Spradling, A. C. (2000). The fusome organizes the microtubule network during oocyte differentiation in Drosophila. Development 127:4253-4264.
39. Guild, G. M., Connelly, P. S., Vranich, K. A., Shaw, M. K., and Tilney, L. G. (2002). Actin filament turnover removes bundles from Drosophila bristle cells. J. Cell Sci. 115:641-653.
40. Halfon, M. S., Gisselbrecht, S., Lu, J., Estrada, B., Keshishian, H., and Michelson, A. M. (2002). New fluorescent protein reporters for use with the Drosophila Gal4 expression system and for vital detection of balancer chromosomes. Genesis 34:135-138.
41. Hazelrigg, T., Liu, N., Hong, Y., and Wang, S. (1998). GFP expression in Drosophila tissues: Time requirements for formation of a fluorescent product. Dev. Biol. 199:245-249.
42. Hiroi, M., Marion-Poll, F., and Tanimura, T. (2002). Differentiated response to sugars among labellar chemosensilla in Drosophila. Zoolog. Sci. 19:1009-1018.
43. Horn, C., and Wimmer, E. A. (2000). A versatile vector set for animal transgenics. Dev. Genes Evol. 201:630-637.
44. Horn, C., Jaunich, B., and Wimmer, E. A. (2000). Highly sensitive, fluorescent transformation marker for Drosophila transgenesis. Dev. Genes Evol. 210:623-629.
45. Huang, J., and Raff, J. W. (1999). The disappearance of cyclin B at the end of mitosis is regulated spatially in Drosophila cells. EMBO J. 18:2184-2195.
46. Hutson, M. S., Tokutake, Y., Chang, M. S., Bloor, J. W., Venakides, S., Kiehart, D. P., and Edwards, G. S. (2003). Forces for morphogenesis investigated with laser microsurgery and quantitative modeling. Science 300:145-149.
47. Ito, K., Suzuki, K., Estes, P., Ramaswami, M., Yamamoto, D., and Strausfeld, N. J. (1998). The organization of extrinsic neurons and their implications in the functional roles of the mushroom bodies in Drosophila melanogaster Meigen. Learn Mem. 5:52-77.
48. Jacinto, A., Wood, W., Balayo, T., Turmaine, M., Martinez-Arias, A., and Martin, P. (2000). Dynamic actin-based epithelial adhesion and cell matching during Drosophila dorsal closure. Curr. Biol. 10:1420-1426.
49. Jacinto, A., Wood, W., Woolner, S., Hiley, C., Turner, L., Wilson, C., Martinez-Arias, A., and Martin, P. (2002). Dynamic analysis of actin cable function during Drosophila dorsal closure. Curr Biol. 12:1245-1250.
50. Jin, Y., Wang, Y., Walker, D. L., Dong, H., Conley, C., Johansen, J., and Johansen, K. M. (1999). JIL-1: A novel chromosomal tandem kinase implicated in transcriptional regulation in Drosophila. Mol. Cell 4:129-135.
51. Kelso, R. J., Buszczak, M., Quinones, A. T., Castiblanco, C., Mazzalupo, S., and Cooley, L. (2004). Flytrap, a database documenting a GFP protein-trap insertion screen in Drosophila melanogaster. Nucleic Acids Res. 32:D418-D420.
52. Kerrebrock, A. W., Moore, D. P., Wu, J. S., and Orr-Weaver, T. L. (1995). Mei-S332, a Drosophila protein required for sister-chromatid cohesion, can localize to meiotic centromere regions. Cell 83:247-256.
53. Kiehart, D. P., Galbraith, C. G., Edwards, K. A., Rickoll, W. L., and Montague, R. A. (2000). Multiple forces contribute to cell sheet morphogenesis for dorsal closure in Drosophila. J. Cell Biol. 149:471-490.
54. Lee, T., and Luo, L. (1999). Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morpholgenesis. Neuron 22:451-461.
55. Lee, T., and Luo, L. (2001). Mosaic analysis with a repressible cell marker (MARCM) for Drosophila neural development. Trends Neurosci. 24:251-254.
56. Lee, M. J., Gergely, F., Jeffers, K., Peak-Chew, S. Y., and Raff, J. W. (2001). Msps/XMAP215 interacts with the centrosomal protein D-TACC to regulate microtubule behaviour. Nat. Cell Biol. 3:643-649.
57. Levashina, E. A., Ohresser, S., Lemaitre, B., and Imler, J. L. (1998). Two distinct pathways can control expression of the gene encoding the Drosophila antimicrobial peptide metchnikowin. J. Mol. Biol. 278:515-527.
58. Martin, S. G., Leclerc, V., Smith-Litiere, K., and St. Johnston, D. (2003). The identification of novel genes required for Drosophila anteroposterior axis formation in a germline clone screen using GFP-Staufen. Development 130:4201-4215.
59. Megraw, T. L., Kilaru, S., Turner, F. R., and Kaufman, T. C. (2002). The centrosome is a dynamic structure that ejects PCM flares. J. Cell Sci. 115:4707-4718.
60. Merabet, S., Catala, F., Pradel, J., and Graba, Y. (2002). A green fluorescent protein reporter genetic screen that identifies modifiers of Hox gene function in the Drosophila embryo. Genetics 162:189-202.
61. Morin, X., Daneman, R., Zavortink, M., and Chia, W. (2001). A protein trap strategy to detect GFP-tagged proteins expressed from their endogenous loci in Drosophila. PNAS 98:15050-15055.
62. Nagel, A. C., Maier, D., and Preiss, A. (2002). Green fluorescent protein as a convenient and versatile marker for studies on functional genomics in Drosophila. Dev. Genes Evol. 212:93-98.
63. Nakamura, A., Amikura, R., Hanyu, K., and Kobayashi, S. (2001). Me31B silences translation of oocyte-localizing RNAs through the formation of cytoplasmic RNP complex during Drosophila oogenesis. Development 128:3233-3242.
64. Neufeld, T. P., de la Cruz, A. F., Johnston, L. A., and Edgar, B. A. (1998). Coordination of growth and cell division in the Drosophila wing. Cell 93:1183-1193.
65. Niswender, K. D., Blackman, S. M., Rohde, L., Magnuson, M. A., and Piston, D. W. (1995). Quantitative imaging of green fluorescent protein in cultured cells: Comparison of microscopic techniques, use in fusion proteins and detection limits. J. Microsc. 180:109-116.
66. Noguchi, T., and Miller, K. G. (2003). A role for actin dynamics in individualization during spermatogenesis in Drosophila melanogaster. Development 130:1805-1816.
67. Parker, L., Gross, S., and Alphey, L. (2001). Vectors for the expression of tagged proteins in Drosophila. Biotechniques 31:1280-1286.
68. Pfeiffer, S., Ricardo, S., Manneville, J. B., Alexandre, C., and Vincent, J. P. (2002). Producing cells retain and recycle Wingless in Drosophila embryos. Curr. Biol. 12:957-962.
69. Plautz, J. D., Day, R. N., Dailey, G. M., Welsh, S. B., Hall, J. C., Halpain, S., and Kay, S. A. (1996). Green fluorescent protein and its derivatives as versatile markers for gene expression in living Drosophila melanogaster, plant and mammalian cells. Gene 173:83-87.
70. Potter, S. M., Wang, C.-W., Garrity, P. A., and Fraser, S. E. (1996). Intravital imaging of green fluorescent protein using two-photon laser-scanning microscopy. Gene 173:25-31.
71. Raff, J. W., Jeffers, K., and Huang, J. Y. (2002). The roles of Fzy/Cdc20 and Fzr/Cdh1 in regulating the destruction of cyclin B in space and time. J. Cell Biol. 157:1139-1149.
72. Rashkova, S., Karam, S. E., Kellum, R., and Pardue, M. L. (2002). Gag proteins of the two Drosophila telomeric retrotransposons are targeted to chromosome ends. J. Cell Biol. 159:397-402.
73. Rashkova, S., Athanasiadis, A., and Pardue, M. L. (2003). Intracellular targeting of Gag proteins of the Drosophila telomeric retrotransposons. J. Virol. 77:6376-6384.
74. Ritzenthaler, S., Suzuki, E., and Chiba, A. (2000). Postsynaptic filopodia in muscle cells interact with innervating motoneuron axons. Nat. Neurosci. 3:1012-1017.
75. Rong, Y. S., and Golic, K. G. (2000). Gene targeting by homologous recombination in Drosophila. Science 288:2013-2018.
76. Salvaterra, P. M., and Kitamoto, T. (2001). Drosophila cholinergic neurons and processes visualized with Gal4/UAS-GFP. Gene. Expr. Patterns 1:73-82.
77. Sano, H., Nakamura, A., and Kobayahsi, S. (2002). Identification of a transcriptional regulatory region for germline-specific expression of vasa gene in Drosophila melanogaster. Mech. Dev. 112:129-139.
78. Savoian, M. S., and Rieder, C. L. (2002). Mitosis in primary cultures of Drosophila melanogaster larval neuroblasts. J. Cell Sci. 115:3061-3072.
79. 2 zinc-finger gene downstream of the circadian clock. Mol. Cell Neurosci 19:501-514.
80. Sepp, K. J., and Auld, V. J. (2003). RhoA and Rac1 GTPases mediate the dynamic rearrangement of actin in peripheral glia. Development 130:1825-1835.
81. Shiga, Y., Tanaka-Matakatsu, M., and Hayashi, S. (1996). A nuclear GFP/β-galactosidase fusion protein as a marker in living Drosophila. Dev. Growth Differ. 38:99-106.
82. Teleman, A. A., and Cohen, S. M. (2000). Dpp gradient formation in the Drosophila wing imaginal disc. Cell 103:971-980.
83. Theodosiou, N. A., and Xu, T. (1998). Use of FLP/FRT system to study Drosophila development. Methods 14:355-365.
84. Theurkauf, W. E., and Hazelrigg, T. I. (1998). In vivo analyses of cytoplasmic transport and cytoskeletal organization during Drosophila oogenesis: Characterization of a multistep anterior localization pathway. Development 125:3655-3666.
85. Timmons, L., Becker, J., Barthmaier, P., Fyrberg, C., Shearn, A., and Fyrberg, E. (1997). Green fluorescent protein/β-galactosidase double reporters for visualizing Drosophila gene expression patterns. Dev. Genet. 20:338-347.
86. Tzou, P., Ohresser, S., Ferrandon, D., Capovilla, M., Reichhart, J. M., Lemaitre, B., Hoffmann, J. A., and Imler, J. L. (2000). Tissue-specific inducible expression of antimicrobial peptide genes in Drosophila surface epithelia. Immunity 13:737-748.
87. Van Roessel, P., Hayward, N. M., Barros, C. S., and Brand, A. H. (2002). Two-color GFP imaging demonstrates cell-autonomy of GAL4-driven RNA interference in Drosophila. Genesis 34:170-173.
88. Vazquez, J., Belmont, A. S., and Sedat, J. W. (2001). Multiple regimes of constrained chromosome motion are regulated in the interphase Drosophila nucleus. Curr. Biol. 11:1227-1239.
89. Vazquez. J., Belmont, A. S., and Sedat, J. W. (2002). The dynamics of homologous chromosome pairing during male Drosophila meiosis. Curr. Biol. 12:1473-1483.
90. Verkhusha, V. V., Tsukita, S., and Oda, H. (1999). Actin dynamics in lamellipodia of migrating border cells in the Drosophila ovary revealed by a GFP-actin fusion protein. FEBS Lett. 445:395-401.
91. Vermaak, D., Hayden, H. S., and Henikoff, S. (2002). Centromere targeting element within the histone fold domain of Cid. Mol. Cell Biol. 22:7553-7561.
92. Wang, S., and Hazelrigg, T. (1994). Implications for bcd mRNA localization from spatial distribution of exu protein in Drosophila oogenesis. Nature (London) 369:400-403.
93. Ward, R. E., Reid, P., Bashirullah, A., D'Avino, P. P., and Thummel, C. S. (2003). GFP in living animals reveals dynamic developmental responses to ecdysone during Drosophila metamorphosis. Dev. Boil. 256:389-402.
94. Wilhelm, J. E., Mansfield, J., Hom-Booher, N., Wang, S., Turck, C. W., Hazelrigg, T., and Vale, R. D. (2000). Isolation of a ribonucleoprotein complex involved in mRNA localization in Drosophila oocytes. J. Cell Biol. 148:427-440.
95. Wood, W., Jacinto, A., Grose, R., Woolner, S., Gale, J., Wilson, C., and Martin, P. (2002). Wound healing recapitulates morphogenesis in Drosophila embryos. Nat. Cell Biol. 4:907-912.
96. Yeh, E., Gustafson, K., and Boulianne, G. L. (1995). Green fluorescent protein as a vital marker and reporter of gene expression in Drosophila. Proc. Natl. Acad. Sci. 92:7036-7040.
97. Zhang, Y. Q., Rodesch, C. K., and Broadie, K. (2002). Living synaptic vesicle marker: Synaptotagmin-GFP. Genesis 34:142-145.