Centrosome and spindle function of the Drosophila Ncd microtubule motor visualized in live embryos using Ncd-GFP fusion proteins

Journal of Cell Science

Volumn 109, Issue 10, 1996, Pages 2429-2442

  Endow, Sharyn A ^a   Komma, Donald J ^a  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

Centrosome; GFP; Kinesin; Microtubule motor; Mitosis; Ncd; Spindle

Indexed keywords

ANAPHASE; ARTICLE; CENTROSOME; CHROMOSOME SEGREGATION; DROSOPHILA; EMBRYO; INTERPHASE; METAPHASE; MICROTUBULE; MITOSIS; MITOSIS SPINDLE; NONHUMAN; PRIORITY JOURNAL;

ADENOSINE TRIPHOSPHATASES; ANIMALS; CELL CYCLE; CENTROSOME; CHROMOSOMES; DROSOPHILA; DROSOPHILA PROTEINS; EMBRYO, NONMAMMALIAN; FEMALE; KINESIN; MICROSCOPY, CONFOCAL; MICROTUBULE PROTEINS; MITOSIS; MITOTIC SPINDLE APPARATUS; MUTATION; RECOMBINANT FUSION PROTEINS; TUBULIN;

AEQUOREA VICTORIA; VICTORIA;

EID: 0029909273     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (37)

1. Axton, J. M., Dombradi, V., Cohen, P. T. and Glover, D. M. (1990). One of the protein phosphatase 1 isoenzymes in Drosophila is essential for mitosis. Cell 63, 33-46.
2. Baker, J., Theurkauf, W. E. and Schubiger, G. (1993). Dynamic changes in microtubule configuration correlate with nuclear migration in the preblastoderm Drosophila embryo. J. Cell Biol. 122, 113-121.
3. Bloom, G. S. and Endow, S. A. (1995). Motor proteins I: kinesins. Protein Profile 2, 1109-1171.
4. Boleti, H., Karsenti, E. and Vernos, I. (1996). Xklp2, a novel Xenopus centrosomal kinesin-like protein required for centrosome separation during mitosis. Cell 84, 49-59.
5. Earnshaw, W. C. and Cooke, C. A. (1991). Analysis of the distribution of the INCENPs throughout mitosis reveals the existence of a pathway of structural changes in the chromosomes during metaphase and early events in cleavage furrow formation. J. Cell Sci. 98, 443-461.
6. Edgar, B. A. and O'Farrell, P. H. (1989). Genetic control of cell division patterns in the Drosophila embryo. Cell 57, 177-187.
7. Endow, S. A., Chandra, R., Komma, D. J., Yamamoto, A. H. and Salmon, E. D. (1994a). Mutants of the Drosophila ncd microtubule motor protein cause centrosomal and spindle pole defects in mitosis. J. Cell Sci. 107, 859-867.
8. Endow, S. A., Kang, S. J., Satterwhite, L. L., Rose, M. D., Skeen, V. P. and Salmon, E. D. (1994b). Yeast Kar3 is a minus-end microtubule motor protein that destabilizes microtubules preferentially at the minus ends. EMBO J. 13, 2708-2713.
9. Gatti, M. and Baker, B. S. (1989). Genes controlling essential cell-cycle functions in Drosophila melanogaster. Genes Dev. 3, 438-453.
10. Glover, D. M. (1989). Mitosis in Drosophila. J. Cell Sci. 92, 137-146.
11. Glover, D. M., Leibowitz, M. H., McLean, D. A. and Parry, H. (1995). Mutations in aurora prevent centrosome separation leading to the formation of monopolar spindles. Cell 81, 95-105.
12. Hatsumi, M. and Endow, S. A. (1992a). The Drosophila ncd microtubule motor protein is spindle-associated in meiotic and mitotic cells. J. Cell Sci. 103, 1013-1020.
13. Hatsumi, M. and Endow, S. A. (1992b). Mutants of the microtubule motor protein, nonclaret disjunctional, affect spindle structure and chromosome movement in meiosis and mitosis. J. Cell Sci. 101, 547-559.
14. Heck, M. M. S., Pereira, A., Pesavento, P., Yannoni, Y., Spradling, A. C. and Goldstein, L. S. B. (1993). The kinesin-like protein KLP61F is essential for mitosis in Drosophila. J. Cell Biol. 123, 665-679.
15. Heim, R., Cubitt, A. B. and Tsien, R. Y. (1995). Improved green fluorescence. Nature 373, 663-664.
16. Hyman, A., Drechsel, D., Kellogg, D., Salser, S., Sawin, K., Steffen, P., Wordeman, L. and Mitchison, T. (1991). Preparation of modified tubulins. Meth. Enzymol. 196, 478-485.
17. Karess, R. E., Chang, X. J., Edwards, K. A., Kulkarni, S., Aguilera, I. and Kiehart, K. P. (1991). The regulatory light chain of nonmuscle myosin is encoded by spaghetti-squash, a gene required for cytokinesis in Drosophila. Cell 65, 1177-1189.
18. Komma, D. J., Horne, A. S. and Endow, S. A. (1991). Separation of meiotic and mitotic effects of claret nondisjunctional on chromosome segregation in Drosophila. EMBO J. 10, 419-424.
19. Lewis, E. B. and Gencarella, W. (1952). Claret and non-disjunction in Drosophila melanogaster. Genetics 37, 600-601.
20. Lindsley, D. L. and Zimm, G. G. (1992). The Genome of Drosophila melanogaster. Academic Press, San Diego.
21. Llamazares, S., Moreira, M. A., Tavares, A., Girdham, C., Gonzalez, C., Karess, R. E., Glover, D. M. and Sunkel, C. E. (1991). polo encodes a protein kinase homologue required for mitosis in Drosophila. Genes Dev. 5, 2153-2164.
22. Lombillo, V. A., Nislow, C., Yen, T. J., Gelfand, V. I. and McIntosh, J. R. (1995a). Antibodies to the kinesin motor domain and CENP-E inhibit microlubule depolymerization-dependent motion of chromosomes in vitro. J. Cell Biol. 128, 107-115.
23. Lombillo, V. A., Stewart, R. J. and McIntosh, J. R. (1995b). Minus-end-directed motion of kinesin-coated microspheres driven by microtubule depolymerization. Nature 373, 161-164.
24. Mayer-Jaekel, R. E., Ohkura, H., Gomes, R., Sunkel, C. E., Baumgartner, S., Hemmings, B. A. and Glover, D. M. (1993). The 55 kd regulatory subunit of Drosophila protein phosphatase 2A is required for anaphase. Cell 72, 621-633.
25. Minden, J. S., Agard, D. A., Sedat, J. W. and Alberts, B. M. (1989) Direct cell lineage analysis in Drosophila melanogaster by time-lapse, three-dimensional optical microscopy of living embryos. J. Cell Biol. 109, 505-516.
26. Nelson, C. R. and Szauter, P. (1992). Timing of mitotic chromosome loss caused by the ncd mutation of Drosophila melanogaster. Cell Motil. Cytoskel. 23, 34-44.
27. Nislow, C., Lombillo, V. A., Kuriyama, R. and McIntosh, J. R. (1992). A plus-end-directed motor enzyme that moves antiparallel microtubules in vitro localizes to the interzone of mitotic spindles. Nature 359, 543-547.
28. Portin, P. (1978). Studies on gynandromorphs induced with the claret-nondisjunctional mutation of Drosophila melanogaster. An approach to the timing of chromosome loss in cleavage mitoses. Heredity 41, 193-203.
29. Sawin, K. E., LeGuellec, K., Philippe, M. and Mitchison, T. J. (1992). Mitotic spindle organization by a plus-end-directed microtubule motor. Nature 359, 540-543.
30. Sturtevant, A. H. (1929). The claret mutant type of Drosophila simulons: a study of chromosome elimination and of cell-lineage. Z. Wiss. Zool. 135, 323-356.
31. Sullivan, W., Minden, J. S. and Alberts, B. M. (1990). daughterless-abo-like, a Drosophila maternal-effect mutation that exhibits abnormal centrosome separation during the late blastoderm divisions. Development 110, 311-323.
32. Theurkauf, W. E. (1992). Behavior of structurally divergent α-tubulin isotypes during Drosophila embryogenesis: evidence for post-translational regulation of isotype abundance. Dev. Biol. 154, 205-217.
33. Valdes-Perez, R. E. and Minden, J. S. (1995). Drosophila melanogaster syncytial nuclear divisions are patterned: time-lapse images, hypothesis and computational evidence. J. Theoret. Biol. 175, 525-532.
34. Walczak, C. E., Mitchison, T. J. and Desai, A. (1996). XKCM1: a Xenopus kinesin-related protein that regulates microtubule dynamics during mitotic spindle assembly. Cell 84, 37-47.
35. Wordeman, L. and Mitchison, T. J. (1995). Identification and partial characterization of mitotic centromere-associated kinesin, a kinesin-related protein that associates with centromeres during mitosis. J. Cell Biol. 128, 95-105.
36. Yamamoto, A. H., Komma, D. J., Shaffer, C. D., Pirrotta, V. and Endow, S. A. (1989). The claret locus in Drosophila encodes products required for eyecolor and for meiotic chromosome segregation. EMBO J. 8, 3543-3552.
37. Yen, T. J., Li, G., Schaar, B. T., Szilak, I. and Cleveland, D. W. (1992). CENP-E is a putative kinetochore motor that accumulates just before mitosis. Nature 359, 536-539.