-
3
-
-
0027488367
-
-
J. Tower, G. H. Karpen, N. Craig, A. C. Spradling, Genetics 133, 347 (1993).
-
(1993)
Genetics
, vol.133
, pp. 347
-
-
Tower, J.1
Karpen, G.H.2
Craig, N.3
Spradling, A.C.4
-
6
-
-
0026418313
-
-
G. B. Gloor, N. A. Nassif, D. M. Johnson-Schlitz, C. R. Preston, W. R. Engels, Science 253, 1110 (1991).
-
(1991)
Science
, vol.253
, pp. 1110
-
-
Gloor, G.B.1
Nassif, N.A.2
Johnson-Schlitz, D.M.3
Preston, C.R.4
Engels, W.R.5
-
12
-
-
0023058299
-
-
L. Colleaux et al., Cell 44, 521 (1986).
-
(1986)
Cell
, vol.44
, pp. 521
-
-
Colleaux, L.1
-
13
-
-
0000113348
-
-
L. Colleaux, L. D'Auriol, F. Galibert, B. Dujon, Proc. Natl. Acad. Sci. U.S.A. 85, 6022 (1988).
-
(1988)
Proc. Natl. Acad. Sci. U.S.A.
, vol.85
, pp. 6022
-
-
Colleaux, L.1
D'Auriol, L.2
Galibert, F.3
Dujon, B.4
-
17
-
-
0023665624
-
-
The 18-bp I-Scel cut site (termed I-site here) (13) was synthesized as two oligonucleotides, GGCCGCTAGGGATAACAGGGTAATGTAC and ATTACCCTGTTATCCCTAGC, that were allowed to anneal to each other and cloned between Not I and Kpn I of plasmid pw8 [R. Klemenz, U. Weber, W. J. Gehring, Nucleic Acids Res. 15, 3947 (1987)]. This generated pP[w8, I-site], the tester construct of Fig. 1A. The same synthetic I-site was cloned between the Not I and Kpn I sites of pP[X97] (39) to generate pP[X97, I-site]. Each of these constructs was transformed by standard P element-mediated techniques. The FRT-flanked portion of P[X97, I-site] was mobilized to the RS3r-4A element on chromosome 2 and to the RS3r-2 element on chromosome 3 by FLP-mediated DNA mobilization (39), generating the tester construct of Fig. 1B in two different locations.
-
(1987)
Nucleic Acids Res.
, vol.15
, pp. 3947
-
-
Klemenz, R.1
Weber, U.2
Gehring, W.J.3
-
18
-
-
0342462731
-
-
note
-
+ loss is measured as the fraction of progeny receiving the reporter chromosome that were white-eyed. For the reporter P[w8, I-site], the results of Fig. 1A are the summed results of testing five independent insertions of the reporter that were located on chromosome X, 2, or 3. For the reporter of Fig. 1B, two independent insertions were tested.
-
-
-
-
21
-
-
0026019344
-
-
H. Sun, N. P. Treco, N. P. Schultes, J. W. Szostak, Cell 64, 1155 (1991).
-
(1991)
Cell
, vol.64
, pp. 1155
-
-
Sun, H.1
Treco, N.P.2
Schultes, N.P.3
Szostak, J.W.4
-
26
-
-
0025895425
-
-
P. Hasty, J. Rivera-Perez, C. Chang, A. Bradley, Mol. Cell. Biol. 11, 4509 (1991).
-
(1991)
Mol. Cell. Biol.
, vol.11
, pp. 4509
-
-
Hasty, P.1
Rivera-Perez, J.2
Chang, C.3
Bradley, A.4
-
28
-
-
0027441115
-
-
P. J. Hastings, C. McGill, B. Shafer, J. N. Strathern, Genetics 135, 973 (1993).
-
(1993)
Genetics
, vol.135
, pp. 973
-
-
Hastings, P.J.1
McGill, C.2
Shafer, B.3
Strathern, J.N.4
-
29
-
-
0027985408
-
-
P. Hasty, M. Crist, M. Grompe, A. Bradley, Mol. Cell. Biol. 14, 8385 (1994).
-
(1994)
Mol. Cell. Biol.
, vol.14
, pp. 8385
-
-
Hasty, P.1
Crist, M.2
Grompe, M.3
Bradley, A.4
-
33
-
-
0342462726
-
-
note
-
For the targeting screen (Fig. 3A), flies with the appropriate genotypes were generated by crossing, heat-shocked during the first 3 days of development, and test-crossed as indicated. Female germ line targeting used two or three females by four males per vial; male germ line targeting used two or three males by four females. In separate experiments, we used one of two transformant lines of the donor construct P[y-donor] that were both located on chromosome 3. We used insertions of 70I-Scel and 70FLP that were located on chromosome 2.
-
-
-
-
35
-
-
0342896793
-
-
note
-
2 (2 df) = 25.6]. In some cases, events from the same vial were molecularly distinct and are reported as independent events.
-
-
-
-
36
-
-
0342896792
-
-
note
-
In addition to Sal I digests for Southern blot analysis, we also carried out Southern blotting after digestion of genomic DNA with Sph I and Eco RI (29).
-
-
-
-
39
-
-
0034708480
-
-
M. D. Adams et al., Science 287, 2185 (2000).
-
(2000)
Science
, vol.287
, pp. 2185
-
-
Adams, M.D.1
-
44
-
-
0030880076
-
-
M. M. Golic, Y. S. Rong, R. B. Petersen, S. L. Lindquist, K. G. Golic, Nucleic Acids Res. 25, 3665 (1997).
-
(1997)
Nucleic Acids Res.
, vol.25
, pp. 3665
-
-
Golic, M.M.1
Rong, Y.S.2
Petersen, R.B.3
Lindquist, S.L.4
Golic, K.G.5
-
48
-
-
0342462721
-
-
note
-
We thank M. Golic and S. Titen for technical assistance, M. Jasin for plasmid pCMV/SCE1XNLS, P. Geyer for plasmid pS/G, E. Raff for the 5-kb genomic fragment of β2t and for plasmid p[β3*] + 3′UTR, and the University of Utah Core Facilities for oligonucleotide synthesis and DNA sequencing. Supported by the University of Utah Research Foundation and by NIH grant R21GM57792.
-
-
-
|