Phenylalanine 90 and 93 are localized within the phenol binding site of human UDP-glucuronosyltransferase 1A10 as determined by photoaffinity labeling, mass spectrometry, and site-directed mutagenesis

Biochemistry

Volumn 45, Issue 7, 2006, Pages 2322-2332

  Xiong, Yan ^a   Bernardi, Dan ^b   Bratton, Stacie ^a   Ward, Michael D ^c   Battaglia, Eric ^b   Finel, Moshe ^d   Drake, Richard R ^c   Radominska Pandya, Anna ^a  

^a UNIVERSITY OF ARKANSAS FOR MEDICAL SCIENCES   (United States)

^b UNIVERSITÉ DE LORRAINE   (France)

^c EASTERN VIRGINIA MEDICAL SCHOOL   (United States)

^d UNIVERSITY OF HELSINKI   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; AROMATIC COMPOUNDS; BENZENE; DESORPTION; IONIZATION; MASS SPECTROMETRY; PHENOLS; SYNTHESIS (CHEMICAL);

4-AZIDO-2-HYDROXYBENZOIC ACID (4-AZHBA); ENZYMATIC DIGESTION; PHENOLIC SUBSTRATES; PHOTOPROBE;

ENZYMES;

4 AZIDO 2 HYDROXYBENZOIC ACID; 4 NITROPHENOL; AMINO ACID; GLUCURONOSYLTRANSFERASE; GLUCURONOSYLTRANSFERASE 1A10; GLUCURONOSYLTRANSFERASE 1A7; GLUCURONOSYLTRANSFERASE 1A8; GLUCURONOSYLTRANSFERASE 1A9; HISTIDINE; HYDROXYBENZOIC ACID DERIVATIVE; HYMECROMONE; METHIONINE; PHENOL; PHENYLALANINE; TRYPSIN; UNCLASSIFIED DRUG; VALINE;

ARTICLE; BINDING SITE; CATALYSIS; ELECTROSPRAY MASS SPECTROMETRY; ENZYME ACTIVITY; GLUCURONIDATION; HUMAN; KINETICS; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NUCLEOTIDE SEQUENCE; PHOTOAFFINITY LABELING; PRIORITY JOURNAL; SEQUENCE ALIGNMENT; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; AZIDES; BINDING SITES; CHROMATOGRAPHY, LIQUID; GLUCURONOSYLTRANSFERASE; HUMANS; HYMECROMONE; KINETICS; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NITROPHENOLS; PHENOL; PHENYLALANINE; PHOTOAFFINITY LABELS; RECOMBINANT PROTEINS; SALICYLIC ACIDS; SEQUENCE ALIGNMENT; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 33144483681     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi0519001     Document Type: Article

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