Expression and characterization of recombinant human UDP-glucuronosyltransferases (UGTs): UGT1A9 is more resistant to detergent inhibition than the other UGTs and was purified as an active dimeric enzyme

Journal of Biological Chemistry

Volumn 278, Issue 6, 2003, Pages 3536-3544

  Kurkela, Mika ^a   Garcia Horsmant, J Arturo ^a,b   Luukkanen, Leena ^a   Mörsky, Saila ^a   Taskinen, Jyrki ^a   Baumann, Marc ^a   Kostiainen, Risto ^a   Hirvonen, Jouni ^a   Finel, Moshe ^a,c  

^a UNIVERSITY OF HELSINKI   (Finland)

^b UNIVERSITY OF EASTERN FINLAND   (Finland)

^c UNIVERSITY OF HELSINKI   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODIES; CELLS; DIMERS; MONOMERS; PHOSPHOLIPIDS;

FUSION PROTEINS;

ENZYMES;

1 NAPHTHOL; ANTIBODY; DETERGENT; DIMER; ENTACAPONE; GLUCURONIDE; GLUCURONOSYLTRANSFERASE; GLUCURONOSYLTRANSFERASE 1A1; GLUCURONOSYLTRANSFERASE 1A3; GLUCURONOSYLTRANSFERASE 1A6; GLUCURONOSYLTRANSFERASE 1A9; GLUCURONOSYLTRANSFERASE 2B7; HEMAGGLUTININ; HISTIDINE; HYBRID PROTEIN; IMIDAZOLE; LIVER ENZYME; MONOMER; PEPTIDE DERIVATIVE; PHOSPHOLIPID; RECOMBINANT ENZYME; SCOPOLETIN; TRITON X 100; UDP GLUCURONOSYLTRANSFERASE 1A4; UDP GLUCURONOSYLTRANSFERASE 2B15; UDP GLUCURONOSYLTRANSFERASE 2B4; UNCLASSIFIED DRUG; DNA; ENZYME INHIBITOR; ISOENZYME; RECOMBINANT PROTEIN;

ANIMAL CELL; ANIMAL CELL CULTURE; ANTIBODY SPECIFICITY; ARTICLE; BACULOVIRUS; CARBOXY TERMINAL SEQUENCE; CELL MEMBRANE; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME BINDING; ENZYME INHIBITION; ENZYME MECHANISM; ENZYME PURIFICATION; EXTRACTION; GLUCURONIDATION; HUMAN; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; INSECT CELL; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN STABILITY; VIRUS INFECTION; AMINO ACID SEQUENCE; CHEMISTRY; DIMERIZATION; DRUG ANTAGONISM; GENETICS; KINETICS; LIQUID CHROMATOGRAPHY; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS;

ANIMALIA; ARACHNIDA; HEXAPODA; INSECTA; UNIDENTIFIED BACULOVIRUS;

AMINO ACID SEQUENCE; BASE SEQUENCE; CHROMATOGRAPHY, LIQUID; CLONING, MOLECULAR; DETERGENTS; DIMERIZATION; DNA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME INHIBITORS; GLUCURONOSYLTRANSFERASE; HUMANS; ISOENZYMES; KINETICS; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS;

EID: 0037423299     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M206136200     Document Type: Article

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