Analysis of opioid binding to UDP-glucuronosyltransferase 2B7 fusion proteins using nuclear magnetic resonance spectroscopy

Molecular Pharmacology

Volumn 59, Issue 6, 2001, Pages 1464-1469

  Coffman, B L ^a   Kearney, W R ^a   Green, M D ^a   Lowery, R G ^a   Tephly, T R ^a  

^a UNIVERSITY OF IOWA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; BUPRENORPHINE; GLUCURONOSYLTRANSFERASE; HYBRID PROTEIN; MORPHINE; NALOXONE; OPIATE; ZIDOVUDINE;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING SITE; DISSOCIATION CONSTANT; DRUG BINDING; DRUG PROTEIN BINDING; DRUG RECEPTOR BINDING; EQUILIBRIUM DIALYSIS; ESCHERICHIA COLI; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN LOCALIZATION;

EID: 0034990314     PISSN: 0026895X     EISSN: None     Source Type: Journal    
DOI: 10.1124/mol.59.6.1464     Document Type: Article

References (26)

1. Small molecule conformation in receptor-bound state by two-dimensional spin exchange experiment
2. Negative nuclear Overhauser effects as probes of macromolecular structure
3. 3′-Azido-3′-deoxythimidine (AZT) is glucuronidated by human UDP-glucuronosyltransferase 2B7 (UGT2B7)
4. Two-dimensional nuclear Overhauser effect in complete relaxation analysis
5. Theoretical evaluation of the two-dimensional transferred nuclear Overhauser effect
6. The glucuronidation of opioids, other xenoblotics and androgens by human UGT2B7Y(268) and UGT2B7H(268)
7. Human UGT2B7 catalyzes morphine glucuronidation
8. NMR studies of flexible opiate confirmations at monoclonal antibody binding sites I. Transferred nuclear Overhauser effects show bound confirmations
9. Glucuronidation of opioids, carboxylic acid-containing drugs, and hydroxylated xenobiotics catalyzed by expressed monkey UDP-glucuuronosyltransferase 2B9 protein
10. Complementary deoxyribonucleic acid cloning and expression of a human liver uridine diphosphate-glucuronosyltransferase glucuronidating carboxylic acid-containing drugs
11. UDP-Glucuronosyltransferases
12. Expression of chimeric cDNAs in cell culture defines a region of UDP glucuronosyltransferase involved in substrate selection
13. The UDP glycosyltransferase gene superfamily: Recommended nomenclature update based on evolutionary divergence
14. Key residues defining the μ-opioid receptor binding pocket: A site-directed mutagenesis study
15. Structure and function of uridine diphosphate glucuronosyltransferases
16. Determinants of UDP glucuronosyltransferase membrane association and residency in the endoplasmic reticulum
17. A model for human cytochrome P450 2D6 based on homology modeling and NMR studies of substrate binding
18. Cloning and expression of solanidine UDP-glucose glucosyltransferase from potato
19. Determination of the human liver UDP-glucuronosyltransferase 2B4 domains involved in the binding of UDP-glucuronic acid using photoaffinity labeling of fusion proteins
20. Structural and functional studies of UDP-glucuronosyltransferases
21. Cloning and expression of human liver UDP-glucuronosyltransferase in COS-1 cells. 3,4-Catechol estrogens and estriol as primary substrates
22. Prediction of protein secondary structure at better than 70% accuracy
23. Ligand recognition in μ opioid receptor: Experimentally based modeling of μ opioid receptor binding sites and their testing by ligand docking
24. 4-Hydroxyretinoic acid, a novel substrate for human liver microsomal UDP-glucurosyltransferase(s) and recombinant UGT2B7
25. Arginine 52 and histidine 54 located in a conserved amino-terminal hydrophobic region (LX2-R52-G-H54-X3-V-L) are important amino acids for the functional and structural integrity of the human liver UDP-glucuronosyltransferase UGT1A6
26. Measurement of spin relaxation in complex systems