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Volumn 16, Issue 7, 2006, Pages 1905-1908

Synthesis of miltirone analogues as inhibitors of Cdc25 phosphatases

Author keywords

Cancer; Cdc25 phosphatases; Miltirone

Indexed keywords

ANTINEOPLASTIC AGENT; MILTIRONE; PROTEIN TYROSINE PHOSPHATASE; PROTEIN TYROSINE PHOSPHATASE INHIBITOR; UNCLASSIFIED DRUG;

EID: 33144467432     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2005.12.080     Document Type: Article
Times cited : (32)

References (41)
  • 39
    • 0039619868 scopus 로고    scopus 로고
    • a,b. The typical inhibition assay for cdc25s was carried out in a 100 μL system containing 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 2 mM DTT, 2 mM EDTA, 1% glycerol, 10 μM OMFP, 2% DMSO, and 200-300 nM GST-cdc25A or 40-80 nM GST-cdc25B. For the PTP1B assay, the reaction system was 50 mM MOPs, pH 6.5, 2 mM DTT, 1 mM EDTA, 2 mM pNPP, 2% DMSO, and 30-40 nM GST-PTP1B. S. Walchli, M.L. Curchod, R.P. Gobert, S. Arkinstall, and R.H.V. Huijsduijnen J. Biol. Chem. 275 2000 9792
    • (2000) J. Biol. Chem. , vol.275 , pp. 9792
    • Walchli, S.1    Curchod, M.L.2    Gobert, R.P.3    Arkinstall, S.4    Huijsduijnen, R.H.V.5
  • 41
    • 33144478621 scopus 로고    scopus 로고
    • note
    • Inhibition of cell proliferation assay: growth inhibition of the tumor cells was analyzed by the MTT method. Human tumor cell lines HCT116 and A549 were trypsined and seeded with 3000 cells/well in 96-well plates, after attaching, cells were treated with different concentration compounds for subsequent 72 h incubation. Then, 40 μL MTT (5 mg/mL) was added into 200 μL medium each well and incubated for 3 h, finally the medium was removed and 500 μL dimethylsulfoxide was added to each well. The absorbance of each well at 550 nM (reference wave length is 690 nM) was determined using SpectraMAX340 microplate reader (Molecular Devices, Sunnyvale, CA).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.