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Inhibition of cell proliferation assay: growth inhibition of the tumor cells was analyzed by the MTT method. Human tumor cell lines HCT116 and A549 were trypsined and seeded with 3000 cells/well in 96-well plates, after attaching, cells were treated with different concentration compounds for subsequent 72 h incubation. Then, 40 μL MTT (5 mg/mL) was added into 200 μL medium each well and incubated for 3 h, finally the medium was removed and 500 μL dimethylsulfoxide was added to each well. The absorbance of each well at 550 nM (reference wave length is 690 nM) was determined using SpectraMAX340 microplate reader (Molecular Devices, Sunnyvale, CA).
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