Expression and purification of extracellular penicillin G acylase in Bacillus subtilis

Protein Expression and Purification

Volumn 21, Issue 1, 2001, Pages 60-64

  Yang, Sheng ^a   Huang, He ^a   Zhang, Ruan ^a   Huang, Xiaodong ^a   Li, Shiyun ^a   Yuan, Zhongyi ^a  

^a INSTITUTE OF BIOCHEMISTRY AND CELL BIOLOGY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

AMIDASE; BETA LACTAM ANTIBIOTIC; CENTRIFUGATION; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME PURIFICATION; EXPRESSION VECTOR; GENETIC REGULATION; GENETIC STRAIN; HYDROPHOBICITY; PENICILLIN G ACYLASE; PHENYLACETIC ACID; POLYMERASE CHAIN REACTION; PROMOTER REGION; TEMPERATURE;

BACILLUS MEGATERIUM; BACILLUS SUBTILIS;

EID: 0034951829     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2000.1339     Document Type: Article

References (10)

1. Penicillin acylase in the industrial production of β-lactam antibiotics
2. Cloning and sequencing of the pac gene encoding the penicillin G acylase of Bacillus megaterium
3. Cloning and expression of penicillin acylase genes from overproducing strains of Escherichia coli and Bacillus megaterium
4. Cloning and expression of the pga gene of Bacillus megaterium in E. coli
5. Expression and Purification of the PGA gene of Bacillus megaterium in Bacillus subtilis
6. High expression of penicillin G acylase gene from Bacilus megaterium in Bacillus subtilis
7. 37 RNA polymerase holoenzymes during growth and stationary phases
8. Formation of competent Bacillus subtilis cells
9. A procedure for the isolation of deoxyribonucleic acid from microorganisms
10. Preparation and general properties of crystalline penicillin acylase from Escherichia coli ATCC11105