Activity screening of carrier domains within nonribosomal peptide synthetases using complex substrate mixtures and large molecule mass spectrometry

Biochemistry

Volumn 45, Issue 6, 2006, Pages 1537-1546

  Dorrestein, Pieter C ^a   Blackhall, Jonathan ^a   Straight, Paul D ^b   Fischbach, Michael A ^b   Garneau Tsodikova, Sylvie ^b   Edwards, Daniel J ^d   McLaughlin, Shaun ^a   Lin, Myat ^a   Gerwick, William H ^c   Kolter, Roberto ^b   Walsh, Christopher T ^b   Kelleher, Neil L ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d CALIFORNIA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASSAYS; FOURIER TRANSFORM INFRARED SPECTROSCOPY; MASS SPECTROMETRY; MOLECULAR DYNAMICS; SCREENING;

ADENYLATION; CARRIER DOMAINS; NONRIBOSOMAL PEPTIDE SYNTHETASES; SUBSTRATE MIXTURES;

PROTEINS;

COUMAMYCIN A1; ENTEROCHELIN; LARGE MOLECULE TRANSPORT AGENT; NIKKOMYCIN; PEPTIDE SYNTHASE; POLYKETIDE SYNTHASE; RIBOSOME PROTEIN; YERSINIABACTIN;

ADENYLATION; ARTICLE; BACILLUS SUBTILIS; BROMINATION; CLUSTER ANALYSIS; COVALENT BOND; DISEASE CLASSIFICATION; ENZYME SUBSTRATE; FOURIER TRANSFORM MASS SPECTROMETRY; GENE CLUSTER; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN DOMAIN; SCREENING;

ADENOSINE TRIPHOSPHATE; AMINOCOUMARINS; AMINOGLYCOSIDES; BACILLUS SUBTILIS; BROMINE; CATALYTIC DOMAIN; ENTEROBACTIN; MASS SPECTROMETRY; MULTIGENE FAMILY; NOVOBIOCIN; PEPTIDE SYNTHASES; PHENOLS; PYRROLIDINONES; SUBSTRATE SPECIFICITY; SULFHYDRYL COMPOUNDS; THIAZOLES;

BACILLUS SUBTILIS;

EID: 32344442373     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi052333k     Document Type: Article

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