Effects of constitutively active GTPases on fibroblast behavior

Cellular and Molecular Life Sciences

Volumn 63, Issue 1, 2006, Pages 82-91

  Zhang, Z G ^a   Lambert, C A ^b   Servotte, S ^b   Chometon, G ^a   Eckes, B ^a   Krieg, T ^a   Lapiere C M ^b   Nusgens, B V ^b   Aumailley, M ^a  

^a UNIVERSITY OF COLOGNE   (Germany)

^b UNIVERSITY OF LIÈGE   (Belgium)

Author keywords

Adhesion; Cdc42; Contraction; Cytoskeleton; Fibroblast; Migration; QL mutants; Rac1; RhoA

Indexed keywords

GUANOSINE TRIPHOSPHATASE; PROTEIN CDC42; RAC1 PROTEIN; RHO GUANINE NUCLEOTIDE BINDING PROTEIN; RHOA GUANINE NUCLEOTIDE BINDING PROTEIN; SCLEROPROTEIN;

ARTICLE; CELL ACTIVITY; CELL ADHESION; CELL CLONING; CELL POPULATION; CELL SPREADING; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SPECIFICITY; FIBROBLAST; HUMAN; HUMAN CELL; PROTEIN EXPRESSION;

ACTINS; CDC42 GTP-BINDING PROTEIN; CELL ADHESION; CELL LINE; CELL MOVEMENT; COLLAGEN; EXTRACELLULAR MATRIX; FIBROBLASTS; HUMANS; RAC1 GTP-BINDING PROTEIN; RHOA GTP-BINDING PROTEIN;

EID: 30744468805     PISSN: 1420682X     EISSN: 15691632     Source Type: Journal    
DOI: 10.1007/s00018-005-5416-5     Document Type: Article

References (48)

1. Hall A. (1998) Rho GTPases and the actin cytoskeleton. Science 279: 509-514
2. Etienne-Manneville S. and Hall A. (2002) Rho GTPases in cell biology. Nature 420: 629-635
3. Krengel U., Schlichting L., Scherer A., Schumann R., Frech M., John J. et al. (1990) Three-dimensional structures of H-ras p21 mutants: molecular basis for their inability to function as signal switch molecules. Cell 62: 539-548
4. Bishop A. L. and Hall A. (2000) Rho GTPases and their effector proteins. Biochem. J. 348: 241-255
5. Burridge K. and Wennerberg K. (2004) Rho and Rac take center stage. Cell 116: 167-179.
6. Ingber D. E. (1997) Tensegrity: the architectural basis of cellular mechanotransduction. Annu. Rev. Physiol. 59: 575-599
7. Grinnell F. (2003) Fibroblast biology in three-dimensional collagen matrices. Trends Cell Biol. 13: 264-269
8. Humphries M. J., Travis M. A., Clark K. and Mould A. P. (2004) Mechanisms of integration of cells and extracellular matrices by integrins. Biochem. Soc. Trans. 32: 822-825
9. Ridley A. J. and Hall A. (1992) The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors. Cell 70: 389-399
10. Ridley A. J., Paterson H. F., Johnston C. L., Diekmann D. and Hall A. (1992) The small GTP-binding protein rac regulates growth factor-induced membrane ruffling. Cell 70: 401-410
11. Hotchin N. A. and Hall A. (1995) The assembly of integrin adhesion complexes requires both extracellular matrix and intracellular rho/rac GTPases. J. Cell Biol. 131: 1857-1865
12. Chrzanowska-Wodnicka M. and Burridge K. (1996) Rho-stimulated contractility drives the formation of stress fibers and focal adhesions. J. Cell Biol. 133: 1403-1415
13. Leeuwen F. N. van, Delft S. van, Kain H. E., Kammen R. A. van der and Collard J. G. (1999) Rac regulates phosphorylation of the myosin-II heavy chain, actinomyosin disassembly and cell spreading. Nat. Cell Biol. 4: 242-248
14. Nobes C. D. and Hall A. (1999) Rho GTPases control polarity, protrusion, and adhesion during cell movement. J. Cell Biol. 144: 1235-1244
15. Raftopoulou M. and Hall A. (2004) Cell migration: Rho GT-Pases lead the way. Dev. Biol. 265: 23-32
16. Servotte S., Zhang Z.-G., Lambert C. A., Ho T. T. G., Chometon G., Eckes B. et al. (2005) Cytoskeletal modifications induced by stable expression of constitutively active Rho-GT-Pases are compatible with fibroblast survival and proliferation. Protoplasma. in press
17. Ren X. D., Kiosses W. B. and Schwartz M. A. (1999) Regulation of the small GTP-binding protein Rho by cell adhesion and the cytoskeleton EMBO J. 18: 578-585
18. Sander E. E., Klooster J. P. ten, Delft S. van, Kammen R. A. van der and Collard J.G. (1999) Rac downregulates Rho activity: reciprocal balance between both GTPases determines cellular morphology and migratory behavior J. Cell Biol. 147: 1009-1022
19. El Mourabit H., Müller S., Tunggal L., Paulsson M. and Aumailley M. (2004) Analysis of the adaptor function of the LIM domain-containing protein FHL2 using an affinity chromatography approach. J. Cell. Biochem. 92: 612-625
20. Tasanen K., Tunggal L., Chometon G., Bruckner-Tuderman L. and Aumailley M. (2004) Keratinocytes from patients lacking collagen XVII display a migratory phenotype. Am. J. Pathol. 164: 2027-2038
21. Rousselle P. and Aumailley M. (1994) Kalinin is more efficient than laminin in promoting adhesion of primary keratinocytes and some other epithelial cells and has a different requirement for integrin receptors J. Cell Biol. 125: 205-214
22. Kessler D., Dethlefsen D., Haase I., Plomann M. F. Hirche F., Krieg T. et al. (2001) Fibroblasts in mechanically stressed collagen lattices assume a 'synthetic' phenotype. J. Biol. Chem. 276: 36575-36585
23. Van Aelst L. and D'Souza-Schorey C. (1997) Rho GTPases and signaling networks. Genes Dev. 11: 2295-2322
24. Nobes C. D. and Hall A. (1995) Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia. Cell 81: 53-62
25. Flier A. van der and Sonnenberg A. (2001) Function and interactions of integrins. Cell Tissue Res. 305: 285-298
26. Arthur W. T. and Burridge K. (2001) RhoA inactivation by p190RhoGAP regulates cell spreading and migration by promoting membrane protrusion and polarity. Mol. Biol. Cell 12: 2711-2720
27. Danen E. H., Sonneveld P., Brakebusch C., Fässler R. and Sonnenberg A. (2002) The fibronectin-binding integrins α5β1 and αvβ3 differentially modulate RhoA-GTP loading, organization of cell matrix adhesions, and fibronectin fibrillogenesis. J. Cell Biol. 159: 1071-1086
28. Moyano J. V., Maqueda A., Casanova B. and Garcia-Pardo A. (2003) α4β1 integrin/ligand interaction inhibits α5β1-induced stress fibers and focal adhesions via down-regulation of RhoA and induces melanoma cell migration. Mol. Biol. Cell. 14: 3699-3715
29. Malliri A. and Collard J. G. (2003) Role of Rho-family proteins in cell adhesion and cancer. Curr. Opin. Cell Biol. 15: 583-589
30. Bell E., Ivarsson B. and Merrill C. (1979) Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro. Proc. Natl. Acad. Sci. USA 76: 1274-1278
31. Mayer T., Meyer M., Janning A., Schiedel A. C. and Barnekow A. (1999) A mutant form of the rho protein can restore stress fibers and adhesion plaques in v-src transformed fibroblasts. Oncogene 18: 2117-2128
32. Michaelson D., Silletti J., Murphy G., D'Eustachio P., Rush M. and Philips M. (2001) Differential localization of Rho GTPases in live cells: regulation by hypervariable regions and RhoGDI binding. J. Cell Biol. 152: 111-126
33. Longenecker K., Read P., Lin S. K., Nakamoto R. K. and Derewenda Z. S. (2003) Structure of a constitutively activated RhoA mutant (Q63L) at 1.55 Angstrom resolution. Acta Cryst. D59: 876-880
34. Kaibuchi K., Kuroda S. and Amano M. (1999) Regulation of the cytoskeleton and cell adhesion by the Rho family GTPases in mammalian cells. Annu. Rev. Biochem. 68: 459-486
35. Stewart M. and Hogg N. (1996) Regulation of leukocyte integrin function: affinity vs. avidity. J. Cell. Biochem. 61: 554-561
36. Bazzoni G. and Hemler M. E. (1998) Are changes in integrin affinity and conformation overemphasized? Trends Biochem. Sci. 23: 30-34
37. Fukata M., Nakagawa M., Kuroda S. and Kaibuchi K. (1999) Cell adhesion and Rho small GTPases J. Cell Sci. 112: 4491-4500
38. Vasioukhin V., Bauer C., Yin M. and Fuchs E. (2000) Directed actin polymerization is the driving force for epithelial cell-cell adhesion. Cell 100: 209-219
39. Kuroda S., Fukata M., Nakagawa M., Fujii K., Nakamura T., Ookubo T. et al. (1998) Role of IQGAP1, a target of the small GTPases Cdc42 and Rac1, in regulation of E-cadherin-mediated cell-cell adhesion. Science 281: 832-835
40. Tkach V., Bock E. and Berezin, V. (2005) The role of RhoA in the regulation of cell morphology and motility. Cell Motil. Cytoskeleton 61: 21-33
41. Gutjahr M.C., Rossy J. and Niggli V. (2005) Role of Rho, Rac, and Rho-kinase in phosphorylation of myosin light chain, development of polarity, and spontaneous migration of Walker 256 carcinosarcoma cells. Exp. Cell Res. 308: 422-438
42. Zhou H.and Kramer R.H. (2005) Integrin engagement differentially modulates epithelial cell motility by RhoA/ROCK and PAK1. J. Biol. Chem. 280: 10624-10635
43. Cox E.A., Sastry S.K. and Huttenlocher A. (2001) Integrin-mediated adhesion regulates cell polarity and membrane protrusion through the Rho family of GTPases. Mol. Biol Cell. 12: 265-277
44. Olivo C., Vanni C., Mancini P., Silengo L., Torrisi M.R., Tarone G. et al. (2000) Distinct involvement of cdc42 and RhoA GTPases in actin organization and cell shape in untransformed and Db1 oncogene transformed NIH3T3 cells. Oncogene 19: 1428-1436
45. Banyard J., Anand-Apte B., Symons M. and Zetter B.R. (2000) Motility and invasion are differentially modulated by Rho family GTPases. Oncogene 19: 580-591
46. Lee D. J., Ho C. H. and Grinnell F. (2003) LPA-stimulated fibroblast contraction of floating collagen matrices does not require Rho kinase activity or retraction of fibroblast extensions. Exp. Cell Res. 289: 86-94
47. Olson M. F. (2004) Contraction reaction: mechanical regulation of Rho GTPase. Trends Cell Biol. 14: 111-114
48. Beningo K. A., Dembo M., Kaverina I., Small J. V. and Wang Y. L. (2001) Nascent focal adhesions are responsible for the generation of strong propulsive forces in migrating fibroblasts. J. Cell Biol. 153: 881-888