Rac regulates phosphorylation of the myosin-II heavy chain, actinomyosin disassembly and cell spreading

Nature Cell Biology

Volumn 1, Issue 4, 1999, Pages 242-248

  Van Leeuwen, Frank N ^a   Van Delft, Sanne ^a   Kain, Hendrie E ^a   Van Der Kammen, Rob A ^a   Collard, John G ^a  

^a NETHERLANDS CANCER INSTITUTE   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

BRADYKININ; CALCIUM; MYOSIN ADENOSINE TRIPHOSPHATASE; MYOSIN HEAVY CHAIN; PROTEIN SERINE THREONINE KINASE; RAC PROTEIN; RHO GUANINE NUCLEOTIDE BINDING PROTEIN;

ANIMAL; ARTICLE; CELL LINE; CELL SIZE; CELL STRAIN; METABOLISM; MOUSE; PHOSPHORYLATION; PHYSIOLOGY; RAT; SIGNAL TRANSDUCTION;

ACTOMYOSIN; ANIMALS; BRADYKININ; CALCIUM; CELL LINE; CELL SIZE; MICE; MYOSIN HEAVY CHAINS; PC12 CELLS; PHOSPHORYLATION; PROTEIN-SERINE-THREONINE KINASES; RAC GTP-BINDING PROTEINS; RATS; RHO GTP-BINDING PROTEINS; SIGNAL TRANSDUCTION;

EID: 0033174147     PISSN: 14657392     EISSN: None     Source Type: Journal    
DOI: 10.1038/12068     Document Type: Article

References (41)

1. Amano, M. et al. Phosphorylation and activation of myosin by Rho-associated kinase (Rho-kinase). J. Biol. Chem. 271, 20246-20249 (1996).
2. Kimura, K. et al. Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase). Science 273, 245-248 (1996).
3. Jalink, K., Hengeveld, T., Morii, N., Narumiya, S. & Moolenaar, W. H. Inhibition of lysophosphatidate- and thrombin-induced neurite retraction and neuronal cell founding by ADP ribosylation of the small GTP-binding protein Rho. J. Cell Biol. 126, 801-810 (1994).
4. Hirose, M. et al. Molecular dissection of the Rho-associated protein kinase (p160ROCK)-regulated neurite remodeling in neuroblastoma N1E-115 cells. J. Cell Biol. 141, 1625-1636 (1998).
5. van Leeuwen, F. N. et al. The guanine nucleotide exchange factor Tiam1 affects neuronal morphology; opposing roles for the small GTPases Rac and Rho. J. Cell Biol. 139, 797-807 (1997).
6. Kozma, R., Sarner, S., Ahmed, S. & Lim, L. Rho family GTPases and neuronal growth cone remodeling: relationship between increased complexity induced by Cdc42 Hs, RaC1 and acetylcholine, and collapse induced by RhoA and lysophosphatidic acid. Mol. Cell. Biol. 17, 1201-1211 (1997).
7. Lim, L., Manser, E., Leung, T. & Hall, C. Regulation of phosphorylation pathways by p21 GTPases. Eur. J. Biochem. 242, 171-185 (1996).
8. Sander, E. E. et al. Matrix-dependent Tiam1/Rac signaling in epithelial cells promotes either cell-cell adhesion or cell migration and is regulated by phosphatidylinositol 3-kinase. J. Cell Biol. 143, 1385-1398 (1998).
9. Manser, E., Leung, T., Salihuddin, H., Zhao, Z. S. & Lim, L. A brain serine/threonine protein kinase activated by Cdc42 and Rac1. Nature 367, 40-46 (1994).
10. Michiels, F., Habets, G. G. M., Stam, J. C., Van der Kammen, R. A. & Collard, J. G. A role for Rac-1 in Tiam1 induced membrane ruffling and T-lymphoma invasion. Nature 375, 338-340 (1995).
11. Kozma, R., Ahmed, S., Best, A. & Lim, L. The Ras-related protein Cdc42HS and Bradykinin promote the formation of peripheral actin microspikes and filopodia in Swiss 3T3 fibroblasts Mol. Cell. Biol. 15, 1942-1952 (1995).
12. Nobes, C. D. & Hall, A. Rho, Rac and Cdc42 GTPases regulate the assembly of multi-molecular focal complexes associated with actin stress fibers, lamellipodia and filopodia. Cell 81, 53-62 (1995).
13. Sells, M. A. et al. Human p21-activated kinase (Pak1) regulates actin organization in mammalian cells. Curr. Biol. 7, 202-210 (1997).
14. Manser, E. et al. Expression of constitutively active alpha-PAK reveals effects of the kinase on actin and focal complexes. Mol. Cell Biol. 17, 1129-1143 (1997).
15. Daniels, R. H., Hall, P. S. & Bokoch, G. M. Membrane targeting of p21-activated kinase 1 (PAK1) induces neurite outgrowth from PC12 cells. EMBO J. 17, 754-764 (1998).
16. Tang , Y. et al. Kinase deficient Pak1 mutants inhibit Ras transformation of Rat-1 fibroblasts. Mol. Cell. Biol. 17, 4454-4464 (1997).
17. King, A. J. et al. The protein kinase Pak3 positively regulates Raf-1 activity through phosphorylation of serine 338. Nature 396, 180-183 (1998).
18. 2+, and ionic fluxes in PC12 cells treated with bradykinin. J. Biol. Chem. 263, 17350-17359 (1988).
19. Zheng, J. Q., Felder, M., Connor, I. A. & Poo, M. M. Turning of nerve growth cones induced by neurotransmitters. Nature 368, 140-144 (1994).
20. Burridge, K. & Chrzanowska-Wodnicka, M. Focal adhesions, contractility, and signaling. Annu. Rev. Cell Dev. Biol. 12, 463-518 (1996).
21. Chrzanowska-Wodnicka, M. & Burridge, K. Rho-stimulated contractility drives the formation of stress fibers and focal adhesions. J. Cell Biol. 133, 1403-1415 (1996).
22. Tan, J. L., Ravid, S. & Spudich, J. A. Control of nonmusde myosins by phosphorylation. Annu. Rev. Biochem. 61, 721-759 (1992).
23. Ludowyke, R. I., Peleg, I., Beaven, M. A. & Adelstein, R. S. Antigen-induced secretion of histamine and the phosphorylation of myosin by protein kinase C in rat basophilic leukemia cells. J. Biol. Chem. 264, 12492-12501 (1989).
24. Spudich, A. Myosin reorganization in activated RBL cells correlates temporally with stimulated secretion. Cell Motil. Cytoskeleton 29, 345-353 (1994).
25. 2+-dependent threonine phosphorylation of myosin heavy chain in rat pancreatic islets and RINm5F cells. J. Biol. Chem. 273, 22729-22737 (1998).
26. Brzeska, H. & Korn, E. D. Regulation of class I and class II myosins by heavy chain phosphorylation. J. Biol. Chem. 271, 16983-16986 (1996).
27. Egelhoff, T. T., Lee, R. J. & Spudich, J. A. Dictyostelium myosin heavy chain phosphorylation sites regulate myosin filament assembly and localization in vivo. Cell 75, 363-371 (1993).
28. Murakami, N., Chauhan, V. P. & Elzinga, M. Two nonmuscle myosin II heavy chain isoforms expressed in rabbit brains: filament forming properties, the effects of phosphorylation by protein kinase C and casein kinase II, and location of the phosphorylation sites. Biochemistry 37, 1989-2003 (1998).
29. Mitchison, T. J. & Cramer, L. P. Actin-based cell motility and cell locomotion. Cell 84, 371-379 (1996).
30. 2+ influx. J. Biol Chem. 271, 7883-7886 (1996).
31. Lee, S. F., Egelhoff, T. T., Mahasneh, A. & Cote, G. P. Cloning and characterization of a Dictyostelium myosin I heavy chain kinase activated by Cdc42 and Rac. J. Biol Chem. 271, 27044-27048 (1996).
32. Brzeska, H., Szczepanowska, J., Hoey, J. & Korn, E. D. The catalytic domain of acanthanioeba myosin I heavy chain kinase. II. Expression of active catalytic domain and sequence homology to p21-activated kinase (PAK). J. Biol. Chem. 271, 27056-27062 (1996).
33. Wu, C. et al. Activation of myosin-I by members of the Ste20p protein family. J. Biol Chem. 271, 31787-31790 (1996).
34. Sanders, L. C., Matsumura, F., Bokoch, G. M. & de Lanerolle, P. Inhibition of myosin light chain kinase by p21-activated kinase. Science 283, 2083-2085 (1999).
35. Sells, M. A., Boyd, J. T. & Chernoff, J. p21-activated kinase 1 (Pak1) regulates cell motility in mammalian fibroblasts. J. Cell Biol. 145, 837-849 (1999).
36. Burridge, K. Crosstalk between Rac and Rho. Science 283, 2028-2029 (1999).
37. Ramos, E., Wysolmerski, R. B. & Masaracchia, R. A. Myosin phosphorylation by human cdc42-dependent S6/H4 kinase/gammaPAK from placenta and lymphoid cells. Recept. Signal Transduct. 7, 99-110 (1997).
38. 2+-independent contraction of smooth muscle. J. Biol Chem. 273, 23433-23439 (1998).
39. Yablonski, D., Kane, L. P., Qian, D. & Weiss, A. A Nck-Pak1 signaling module is required for T-cell receptor-mediated activation of NFAT, but not of JNK. EMBO J. 17, 5647-5657 (1998).
40. Kinsella, T. M. & Nolan, G. P. Episomal vectors rapidly and stably produce high-liter recombinant retrovirus. Hum. Gene Ther. 7, 1405-1413 (1996).
41. Kelley, C. A. et al. Xenopus nonmuscle myosin heavy chain isoforms have different subcellular localizations and enzymatic activities J. Cell Biol. 134, 675-687 (1996).