The receptor-Gα fusion protein as a tool for ligand screening: A model study using a nociceptin receptor-Gαi2 fusion protein

Journal of Biochemistry

Volumn 135, Issue 5, 2004, Pages 597-604

  Takeda, Shigeki ^a,b,e   Okada, Tomoaki ^c,f   Okamura, Michiko ^a   Haga, Tatsuya ^a,b,c   Isoyama Tanaka, Junko ^d   Kuwahara, Hiromiki ^d   Minamino, Naoto ^d  

^a UNIVERSITY OF TOKYO   (Japan)

^b GAKUSHUIN UNIVERSITY   (Japan)

^c JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^d NATIONAL CEREBRAL AND CARDIOVASCULAR CENTER   (Japan)

^e GUNMA UNIVERSITY   (Japan)

^f NARA INSTITUTE OF SCIENCE AND TECHNOLOGY   (Japan)

Author keywords

Endogenous ligands; G protein coupled receptor; High throughput screening; Nociceptin

Indexed keywords

BRAIN EXTRACT; G PROTEIN COUPLED RECEPTOR; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE 5' O (3 THIOTRIPHOSPHATE); GUANOSINE DIPHOSPHATE; HYBRID PROTEIN; INHIBITORY GUANINE NUCLEOTIDE BINDING PROTEIN ALPHA2 SUBUNIT; LIGAND; NOCICEPTIN; NOCICEPTIN RECEPTOR; NOCICEPTIN RECEPTOR AGONIST; NOCICEPTIN RECEPTOR ANTAGONIST; PARTIAL AGONIST; UNCLASSIFIED DRUG; INHIBITORY GUANINE NUCLEOTIDE BINDING PROTEIN; ONCOPROTEIN; OPIATE PEPTIDE; OPIATE RECEPTOR; PRIMER DNA;

ANIMAL TISSUE; ARTICLE; BINDING AFFINITY; CONCENTRATION (PARAMETERS); HIGH THROUGHPUT SCREENING; IC 50; LIGAND BINDING; MEDIAN EFFECTIVE CONCENTRATION; MODEL; NONHUMAN; PROTEIN BINDING; SCREENING TEST; SWINE; ANIMAL; BIOCHEMISTRY; BRAIN; CHEMISTRY; CHROMATOGRAPHY; DOSE RESPONSE; KINETICS; METABOLISM; METHODOLOGY;

ANIMALIA; SUS SCROFA;

ANIMALS; BIOCHEMISTRY; BRAIN; CHROMATOGRAPHY; DNA PRIMERS; DOSE-RESPONSE RELATIONSHIP, DRUG; GTP-BINDING PROTEIN ALPHA SUBUNIT, GI2; GTP-BINDING PROTEIN ALPHA SUBUNITS, GI-GO; GUANOSINE 5'-O-(3-THIOTRIPHOSPHATE); KINETICS; LIGANDS; OPIOID PEPTIDES; PROTEIN BINDING; PROTO-ONCOGENE PROTEINS; RECEPTORS, OPIOID; RECOMBINANT FUSION PROTEINS; SWINE;

EID: 3042711567     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/jb/mvh073     Document Type: Article

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