Blue native/SDS-PAGE analysis reveals reduced expression of the mCICA3 protein in cystic fibrosis knock-out mice

Molecular and Cellular Proteomics

Volumn 4, Issue 11, 2005, Pages 1762-1775

  Brouillard, Franck ^a,b   Bensalem, Noura ^a   Hinzpeter, Alexandre ^a,b   Tondelier, Danielle ^a,b   Trudel, Stéphanie ^a,b   Gruber, Achim D ^c,d   Ollero, Mario ^a,b   Edelman, Aleksander ^a,b  

^a INSERM   (France)

^b UNIVERSITÉ PARIS DESCARTES   (France)

^c UNIVERSITY OF VETERINARY MEDICINE HANNOVER   (Germany)

^d FREIE UNIVERSITÄT BERLIN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CHLORIDE CHANNEL; NIFLUMIC ACID; TRANSMEMBRANE CONDUCTANCE REGULATOR;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; AUTOSOMAL RECESSIVE DISORDER; CELL MEMBRANE; COLON MUCOSA; CONTROLLED STUDY; CORRELATION ANALYSIS; CYSTIC FIBROSIS; EX VIVO STUDY; GENE EXPRESSION REGULATION; GENE MUTATION; GOBLET CELL; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; KNOCKOUT MOUSE; LUNG ALVEOLUS EPITHELIUM; MALE; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MITOCHONDRION; MOUSE; MOUSE STRAIN; MUCUS SECRETION; NONHUMAN; NUCLEOTIDE SEQUENCE; PLEIOTROPY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEOMICS; STATISTICAL SIGNIFICANCE;

AMINO ACID SEQUENCE; ANIMALS; CARBACHOL; CHLORIDE CHANNELS; COLON; CYSTIC FIBROSIS; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLYCOPROTEINS; LUNG; MALE; MEMBRANE PROTEINS; MICE; MICE, INBRED C57BL; MICE, KNOCKOUT; MOLECULAR SEQUENCE DATA; MUCOPROTEINS; NIFLUMIC ACID; PROTEOMICS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; STAINING AND LABELING; TIME FACTORS;

ANIMALIA; MURINAE;

EID: 28644438680     PISSN: 15359476     EISSN: None     Source Type: Journal    
DOI: 10.1074/mcp.M500098-MCP200     Document Type: Article

References (53)

1. Vankeerberghen, A., Cuppens, H., and Cassiman, J. (2002) The cystic fibrosis transmembrane conductance regulator: an intriguing protein with pleiotropic functions. J. Cyst. Fibros. 1, 13-29
2. McAuley, D., and Elborn, J. (2000) Cystic fibrosis: basic science. Paediatr. Respir. Rev. 1, 93-100
3. Short, D., Trotter, K., Reczek, D., Kreda, S., Bretscher, A., Boucher, R. C., Stutts, M. J., and Milgram, S. L. (1998) An apical PDZ protein anchors the cystic fibrosis transmembrane conductance regulator to the cytoskeleton. J. Biol. Chem. 273, 19797-19801
4. Li, C., Roy, K., Dandridge, K., and Naren, A. (2004) Molecular assembly of cystic fibrosis transmembrane conductance regulator in plasma membrane. J. Biol. Chem. 279, 24673-24684
5. Davies, J., Griesenbach, U., and Alton, E. (2005) Modifier genes in cystic fibrosis. Pediatr. Pulmonol. 39, 383-391
6. Ritzka, M., Stanke, F., Jansen, S., Gruber, A., Pusch, L., Woelfl, S., Veeze, H. J., Halley, D. J., and Tummler, B. (2004) The CLCA gene locus as a modulator of the gastrointestinal basic defect in cystic fibrosis. Hum. Genet. 115, 483-491
7. Bensalem, N., Ventura, A. P., Vallee, B., Lipecka, J., Tondelier, D., Davezac, N., Dos Santos, A., Perretti, M., Fajac, A., Sermet-Gaudelus, I., Renouil, M., Lesure, J.-F., Halgand, F., Laprevote, O., and Edelman, A. (July 12, 2005) Down-regulation of the anti-inflammatory protein annexin A1 in cystic fibrosis knock-out mice and patients. Mol. Cell. Proteomics 10.1074/mcp.M500019-MCP200
8. Santoni, V., Molloy, M., and Rabilloud, T. (2000) Membrane proteins and proteomics: un amour impossible? Electrophoresis 21, 1054-1070
9. Henningsen, R., Gale, B., Straub, K., and DeNagel, D. (2002) Application of zwitterionic detergents to the solubilization of integral membrane proteins for two-dimensional gel electrophoresis and mass spectrometry. Proteomics 2, 1479-1488
10. Wu, C. C., Yates, J. R., III (2003) The application of mass spectrometry to membrane proteomics. Nat. Biotechnol. 21, 262-267
11. Schägger, H., and von Jagow, G. (1991) Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form. Anal. Biochem. 199, 223-231
12. Bentlage, H., Coo, R. D., Laak, H. T., Sengers, R., Trijbels, F., Ruitenbeek, W., Schlote, W., Pfeiffer, K., Gencic, S., and von Jagow, G. (1995) Human diseases with defects in oxidative phosphorylation. 1. Decreased amounts of assembled oxidative phosphorylation complexes in mitochondrial encephalomyopathies. Eur. J. Biochem. 227, 909-915
13. Schägger, H. (1995) Quantification of oxidative phosphorylation enzymes after blue native electrophoresis and two-dimensional resolution: normal complex I protein amounts in Parkinson's disease conflict with reduced catalytic activities. Electrophoresis 16, 763-770
14. Schägger, H., and Ohm, T. (1995) Human diseases with defects in oxidative phosphorylation. 2. F1F0 ATP-synthase defects in Alzheimer disease revealed by blue native polyacrylamide gel electrophoresis. Eur. J. Biochem. 227, 916-921
15. Yoshinaka, K., Kumanogoh, H., Nakamura, S., and Maekawa, S. (2004) Identification of V-ATPase as a major component in the raft fraction prepared from the synaptic plasma membrane and the synaptic vesicle of rat brain. Neurosci. Lett. 363, 168-172
16. Wang, L., and Dobberstein, B. (1999) Oligomeric complexes involved in translocation of proteins across the membrane of the endoplasmic reticulum. FEES Lett. 457, 316-322
17. Claeys, D., Geering, K., and Meyer, B. (2005) Two-dimensional blue native/sodium dodecyl sulfate gel electrophoresis for analysis of multimeric proteins in platelets. Electrophoresis 26, 1189-1199
18. Snouwaert, J., Brigman, K., Latour, A., Malouf, N., Boucher, R., Smithies, O., and Koller, B. (1992) An animal model for cystic fibrosis made by gene targeting. Science 257, 1083-1088
19. Constantine, S., Au, V., and Slavotinek, J. (2004) Abdominal manifestations of cystic fibrosis in adults: a review. Australas. Radiol. 48, 450-458
20. Dorin, J., Dickinson, P., Alton, E., Smith, S., Geddes, D., Stevenson, B. J., Kimber, W. L., Fleming, S., Clarke, A. R., Hooper, M. L., Anderson, L., Beddington, R. S. P., and Porteous, D. J. (1992) Cystic fibrosis in the mouse by targeted insertional mutagenesis. Nature 359, 211-215
21. Kreiselmeier, N., Kraynack, N., Corey, D., and Kelley, T. (2003) Statin-mediated correction of STAT1 signaling and inducible nitric oxide synthase expression in cystic fibrosis epithelial cells. Am. J. Physiol. 285, L1286-L1295
22. Shevchenko, A., Wilm, M., Vorm, O., and Mann, M. (1996) Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal. Chem. 68, 850-858
23. Leverkoehne, I., and Gruber, A. (2002) The murine mCLCA3 (alias gob-5) protein is located in the mucin granule membranes of intestinal, respiratory, and uterine goblet cells. J. Histochem. Cytochem. 50, 829-838
24. Lipecka, J., Bali, M., Thomas, A., Fanen, P., Edelman, A., and Fritsch, J. (2002) Distribution of ClC-2 chloride channel in rat and human epithelial tissues. Am. J. Physiol. 282, C805-C816
25. Steinberg, T., Pretty On Top, K., Berggren, K., Kemper, C., Jones, L., Diwu, Z., Haugland, R., and Patton, W. (2001) Rapid and simple single nanogram detection of glycoproteins in polyacrylamide gels and on electroblots. Proteomics 1, 841-855
26. Nakanishi, A., Morita, S., Iwashita, H., Sagiya, Y., Ashida, Y., Shirafuji, H., Fujisawa, Y., Nishimura, O., and Fujino, M. (2001) Role of gob-5 in mucus overproduction and airway hyperresponsiveness in asthma. Proc. Natl. Acad. Sci. U. S. A. 98, 5175-5180
27. - channel 1 (CaCC1) gene in the asthmatic airway. Am. J. Respir. Crit Care Med. 165, 1132-1136
28. - channels in Xenopus oocytes. Mol. Pharmacol. 37, 720-724
29. Romio, L., Musante, L., Cinti, R., Seri, M., Moran, O., Zegarra-Moran, O., and Galietta, L. (1999) Characterization of a murine gene homologous to the bovine CaCC chloride channel. Gene (Amst.) 228, 181-188
30. Carew, M., Yang, X., Schultz, C., and Shears, S. (2000) myo-Inositol 3,4,5,6-tetrakisphosphate inhibits an apical calcium-activated chloride conductance in polarized monolayers of a cystic fibrosis cell line. J. Biol. Chem. 275, 26906-26913
31. - channels: a newly emerging anion transport family. Pfluegers Arch. 443, Suppl. 1, S107-S110
32. Zhou, Y., Shapiro, M., Dong, Q., Louahed, J., Weiss, C., Wan, S., Chen, Q., Dragwa, C., Savio, D., Huag, M., Fuller, C., Tomer, Y., Nicolaides, N., McLane, M., and Levitt, R. (2002) A calcium-activated chloride channel blocker inhibits goblet cell metaplasia and mucus overproduction. Novartis Found. Symp. 248, 150-165
33. Hauber, H., Daigneault, P., Frenkiel, S., Lavigne, F., Hung, H., Levitt, R., and Hamid, Q. (2005) Niflumic acid and MSI-2216 reduce TNF-α-induced mucin expression in human airway mucosa. J. Allergy Clin. Immunol. 115, 266-271
34. Kamijo, A., Terakawa, S., and Hisamatsu, K. (1993) Neurotransmitter-induced exocytosis in goblet and acinar cells of rat nasal mucosa studied by video microscopy. Am. J. Physiol. 265, L200-L209
35. Segawa, A., Terakawa, S., Yamashina, S., and Hopkins, C. (1991) Exocytosis in living salivary glands: direct visualization by video-enhanced microscopy and confocal laser microscopy. Eur. J. Cell Biol. 54, 322-330
36. Halm, D., and Halm, S. (2000) Secretagogue response of goblet cells and columnar cells in human colonic crypts. Am. J. Physiol. 278, C212-C233
37. Ma, T., Thiagarajah, J., Yang, H., Sonawane, N., Folli, C., Galietta, L., and Verkman, A. (2002) Thiazolidinone CFTR inhibitor identified by high-throughput screening blocks cholera toxin-induced intestinal fluid secretion. J. Clin. Investig. 110, 1651-1658
38. Brunger, A., and DeLaBarre, B. (2003) NSF and p97/VCP: similar at first, different at last. FEBS Lett. 555, 126-133
39. Rabouille, C., Levine, T., Peters, J., and Warren, G. (1995) An NSF-like ATPase, p97, and NSF mediate cisternal regrowth from mitotic Golgi fragments. Cell 82, 905-914
40. Pleasure, I., Black, M., and Keen, J. (1993) Valosin-containing protein, VCP, is a ubiquitous clathrin-binding protein. Nature 365, 459-462
41. Llorca, O., McCormack, E., Hynes, G., Grantham, J., Cordell, J., Carrascosa, J., Willison, K., Fernandez, J., and Valpuesta, J. (1999) Eukaryotic type II chaperonin CCT interacts with actin through specific subunits. Nature 402, 693-696
42. Creutz, C., Liou, A., Snyder, S., Brownawell, A., and Willison, K. (1994) Identification of the major chromaffin granule-binding protein, chromobindin A, as the cytosolic chaperonin CCT (chaperonin containing TCP-1). J. Biol. Chem. 269, 32035-32038
43. Loewen, M., and Forsyth, G. (2005) Structure and function of CLCA proteins. Physiol. Rev. 85, 1061-1092
44. 2+-mediated Cl secretion in nasal epithelia of CF mice. Am. J. Physiol. 266, C1479-C1483
45. Rozmahel, R., Wilschanski, M., Matin, A., Plyte, S., Oliver, M., Auerbach, W., Moore, A., Forstner, J., Durie, P., Nadeau, J., Bear, C., and Tsui, L. (1996) Modulation of disease severity in cystic fibrosis transmembrane conductance regulator deficient mice by a secondary genetic factor. Nat. Genet. 12, 280-287
46. Clarke, L., Grubb, B., Yankaskas, J., Cotton, C., McKenzie, A., and Boucher, R. (1994) Relationship of a non-cystic fibrosis transmembrane conductance regulator-mediated chloride conductance to organ-level disease in Cftr(-/-) mice. Proc. Natl. Acad. Sci. U. S. A. 91, 479-483
47. Gandhi, R., Elble, R., Gruber, A., Schreur, K., Ji, H., Fuller, C., and Pauli, B. (1998) Molecular and functional characterization of a calcium-sensitive chloride channel from mouse lung. J. Biol. Chem. 273, 32096-32101
48. Wilschanski, M., Rozmahel, R., Beharry, S., Kent, G., Li, C., Tsui, L., Durie, P., and Bear, C. (1996) In vivo measurements of ion transport in long-living CF mice. Biochem. Biophys. Res. Commun. 219, 753-759
49. Podolsky, D. (1989) The colonic goblet cell and glycoprotein heterogeneity. Immunol. Investig. 18, 485-497
50. Cuthbert, A., MacVinish, L., Hickman, M., Ratcliff, R., Colledge, W., and Evans, M. (1994) Ion-transporting activity in the murine colonic epithelium of normal animals and animals with cystic fibrosis. Pfluegers Arch. 428, 508-515
51. Hasty, P., O'Neal, W., Liu, K., Morris, A., Bebok, Z., Shumyatsky, G., Jilling, T., Sorscher, E., Bradley, A., and Beaudet, A. (1995) Severe phenotype in mice with termination mutation in exon 2 of cystic fibrosis gene. Somat. Cell Mol. Genet. 21, 177-187
52. Grubb, B. (1997) Ion transport across the murine intestine in the absence and presence of CFTR. Comp. Biochem. Physiol. A Physiol. 118, 277-282
53. Pappin, D., Hojrup, P., and Bleasby, A. (1993) Rapid identification of proteins by peptide-mass fingerprinting. Curr. Biol. 3, 327-332