Application of zwitterionic detergents to the solubilization of integral membrane proteins for two-dimensional gel electrophoresis and mass spectrometry

Proteomics

Volumn 2, Issue 11, 2002, Pages 1479-1488

  Henningsen, Robert ^a   Gale, Brenda L ^b   Straub, Kenneth M ^b   DeNagel, Diane C ^a,b  

^a Neurobiology Unit   (United States)

^b ROCHE BIOSCIENCE   (United States)

Author keywords

Mass spectrometry; Membrane protein; Two dimensional gel electrophoresis; Zwitterionic detergent

Indexed keywords

3 [(3 CHOLAMIDOPROPYL)DIMETHYLAMINO] 1 PROPANESULFONATE; 4 OCTYLBENZOL AMIDOSULFOBETAINE; AMPHOLYTE; ANAZOLENE SODIUM; DETERGENT; G PROTEIN COUPLED RECEPTOR; HISTAMINE H2 RECEPTOR; ION CHANNEL; MEMBRANE PROTEIN; MYRISTIC AMIDOSULFOBETAINE; PURINE P2X RECEPTOR; SILVER; UNCLASSIFIED DRUG; PEPTIDE FRAGMENT; PURINE P2 RECEPTOR; PURINE P2X3 RECEPTOR;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IMMUNOBLOTTING; LIQUID CHROMATOGRAPHY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN INTERACTION; PROTEIN LOCALIZATION; PROTEOMICS; TANDEM MASS SPECTROMETRY; TWO DIMENSIONAL GEL ELECTROPHORESIS; WESTERN BLOTTING; AMINO ACID SEQUENCE; ANIMAL; CHEMISTRY; CHO CELL; HAMSTER; MASS SPECTROMETRY; METHODOLOGY; SOLUBILITY;

ANIMALIA;

AMINO ACID SEQUENCE; ANIMALS; CHO CELLS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CRICETINAE; DETERGENTS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ION CHANNELS; MEMBRANE PROTEINS; PEPTIDE FRAGMENTS; RECEPTORS, PURINERGIC P2; SOLUBILITY; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 0036857488     PISSN: 16159853     EISSN: None     Source Type: Journal    
DOI: 10.1002/1615-9861(200211)2:11<1479::AID-PROT1479>3.0.CO;2-A     Document Type: Article

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