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The concentration of the channel in the vesicles is reported as mol % (with respect to the lipid); the lipid content was estimated by using a modification of the Bartlett assay (details in the Supporting Information).
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The activity relative to gramicidin A was gauged using k values obtained for 0.078 mol % of gramicidin A and 0.04 mol % of 1 as gramicidin A forms bimolecular ion channels.
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A lower channel concentration (0.3 mol %) was used in this case to obtain a curve amenable to fitting.
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