Effect of temperature and ATP supply on the efficiency of programmed nonsense suppression

Analytical Biochemistry

Volumn 345, Issue 1, 2005, Pages 110-115

  Ahn, Jin Ho ^a   Kim, Nam Young ^a   Kim, Tae Wan ^a   Son, Jeong Mi ^a   Kang, Taek Jin ^a   Park, Chang Gil ^b   Choi, Cha Yong ^a   Kim, Dong Myung ^b  

^a Seoul National University   (South Korea)

^b Chungnam National University   (South Korea)

Author keywords

ATP regeneration; Cell free protein synthesis; Erythropoietin; In vitro protein synthesis; Nonsense suppression; Translation

Indexed keywords

AMINO ACIDS; EFFICIENCY; ELONGATION; MOLECULES; PEPTIDES; TRANSLATION (LANGUAGES);

CELL FREE PROTEIN SYNTHESIS; EFFECT OF TEMPERATURE; ERYTHROPOIETIN (EPO); HUMAN ERYTHROPOIETIN; IN-VITRO; NONSENSE SUPPRESSION; REACTION TEMPERATURE; UNNATURAL AMINO ACIDS;

BIOSYNTHESIS;

2 NITROBENZYLCYSTEINE; ADENOSINE TRIPHOSPHATE; CYSTEINE DERIVATIVE; ERYTHROPOIETIN; UNCLASSIFIED DRUG;

ARTICLE; IN VITRO STUDY; LOW TEMPERATURE; PRIORITY JOURNAL; RNA TRANSLATION; TEMPERATURE SENSITIVITY;

EID: 27644596317     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2005.07.010     Document Type: Article

References (31)

1. S.N. Cook, W.E. Jack, X.F. Xiong, L.E. Danley, J.A. Ellman, P.G. Schultz, C.J. Noren, Photochemically initiated protein splicing, Angew. Chem. Int. Edit. 34 (1995) 1629-1630.
2. P.M. England, H.A. Lester, N. Davidson, D.A. Dougherty, Site-specific, photochemical proteolysis applied to ion channels in vivo, Proc. Natl. Acad. Sci. USA 94 (1997) 11025-11030.
3. J.C. Miller, S.K. Silverman, P.M. England, D.A. Dougherty, H.A. Lester, Flash decaging of tyrosine sidechains in an ion channel, Neuron 20 (1998) 619-624.
4. S.K. Pollitt, P.G. Schultz, A photochemical switch for controlling protein-protein interactions, Angew. Chem. Int. Edit. 37 (1998) 2104-2107.
5. G. Turcatti, K. Nemeth, M.D. Edgerton, U. Meseth, F. Talabot, M. Peitsch, J. Knowles, H. Vogel, A. Chollet, Probing the structure and function of the tachykinin neurokinin-2 receptor through biosynthetic incorporation of fluorescent amino acids at specific sites, J. Biol. Chem. 271 (1996) 19991-19998.
6. L.E. Steward, C.S. Collins, M.A. Gilmore, J.E. Carlson, J.B.A. Ross, A.R. Chamberlin, In vitro site-specific incorporation of fluorescent probes into beta-galactosidase, J. Am. Chem. Soc. 119 (1997) 6-11.
7. B.E. Cohen, T.B. McAnaney, E.S. Park, Y.N. Jan, S.G. Boxer, L.Y. Jan, Probing protein electrostatics with a synthetic fluorescent amino acid, Science 296 (2002) 1700-1703.
8. M. Taki, T. Hohsaka, H. Murakami, K. Taira, M. Sisido, Position-specific incorporation of a fluorophore-quencher pair into a single streptavidin through orthogonal four-base codon/anticodon pairs, J. Am. Chem. Soc. 124 (2002) 14586-14590.
9. V.W. Cornish, D.R. Benson, C.A. Altenbach, K. Hideg, W.L. Hubbell, P.G. Schultz, Site-Specific incorporation of biophysical probes into proteins, Proc. Natl. Acad. Sci. USA 91 (1994) 2910-2914.
10. V.W. Cornish, K.M. Hahn, P.G. Schultz, Site-specific protein modification using a ketone handle, J. Am. Chem. Soc. 118 (1996) 8150-8151.
11. L. Wang, Z.W. Zhang, A. Brock, P.G. Schultz, Addition of the keto functional group to the genetic code of Escherichia coli, Proc. Natl. Acad. Sci. USA 100 (2003) 56-61.
12. J.T. Koh, V.W. Cornish, P.G. Schultz, An experimental approach to evaluating the role of backbone interactions in proteins using unnatural amino acid mutagenesis, Biochemistry 36 (1997) 11314-11322.
13. P.M. England, Y. Zhang, D.A. Dougherty, H.A. Lester, Backbone mutations in transmembrane domains of a ligand-gated ion channel: implications for the mechanism of gating, Cell 96 (1999) 89-98.
14. J.S. Thorson, I. Shin, E. Chapman, G. Stenberg, B. Mannervik, P.G. Schultz, Analysis of the role of the active site tyrosine in human glutathione transferase A1-1 by unnatural amino acid mutagenesis, J. Am. Chem. Soc. 120 (1998) 451-452.
15. K. Kawashima, M. Sisido, K. Ichimura, Photocontrolled attachment of anti-azobenzene antibody onto azobenzene-linked quartz surfaces, Chem. Lett. (1995) 491-492.
16. B.X. Wang, K.C. Brown, M. Lodder, C.S. Craik, S.M. Hecht, Chemically mediated site-specific proteolysis. Alteration of protein-protein interaction, Biochemistry 41 (2002) 2805-2813.
17. H.H. Chung, D.R. Benson, V.W. Cornish, P.G. Schultz, Probing the role of loop 2 in Ras function with unnatural amino acids, Proc. Natl. Acad. Sci. USA 90 (1993) 10145-10149.
18. H.H. Chung, D.R. Benson, P.G. Schultz, Probing the structure and mechanism of Ras protein with an expanded genetic code, Science 259 (1993) 806-809.
19. L. Wang, A. Brock, B. Herberich, P.G. Schultz, Expanding the genetic code of Escherichia coli, Science 292 (2001) 498-500.
20. J.W. Chin, T.A. Cropp, J.C. Anderson, M. Mukherji, Z.W. Zhang, P.G. Schultz, An expanded eukaryotic genetic code, Science 301 (2003) 964-967.
21. J. Ellman, D. Mendel, S. Anthony-Cahill, C.J. Noren, P.G. Schultz, Biosynthetic method for introducing unnatural amino acids site-specifically into proteins, Methods Enzymol. 202 (1991) 301-336.
22. K. Ninomiya, T. Kurita, T. Hohsaka, M. Sisido, Facile aminoacylation of pdCpA dinucleotide with a nonnatural amino acid in cationic micelle, Chem. Commun. (2003) 2242-2243.
23. C.H. Rohrig, O.A. Retz, T. Meergans, R.R. Schmidt, In vitro non-natural amino acid mutagenesis using a suppressor tRNA generated by the cis-acting hepatitis delta virus ribozyme, Biochem. Biophys. Res. Co. 325 (2004) 731-738.
24. P. Davanloo, A.H. Rosenberg, J.J. Dunn, F.W. Studier, Cloning and expression of the gene for bacteriophage T7 RNA polymerase, Proc. Natl. Acad. Sci. USA 81 (1984) 2035-2039.
25. D.M. Kim, T. Kigawa, C.Y. Choi, S. Yokoyama, A highly efficient cell-free protein synthesis system from Escherichia coli, Eur. J. Biochem. 239 (1996) 881-886.
26. T.J. Kang, J.H. Woo, H.K. Song, J.H. Ahn, J.W. Kum, J. Han, C.Y. Choi, H. Joo, A cell-free protein synthesis system as an investigational tool for the translation stop processes, FEBS Lett. 517 (2002) 211-214.
27. D.M. Kim, J.R. Swartz, Prolonging cell-free protein synthesis with a novel ATP regeneration system, Biotechnol. Bioeng. 66 (1999) 180-188.
28. R.G. Kim, C.Y. Choi, Expression-independent consumption of substrates in cell-free expression system from Escherichia coli, J. Biotechnol. 84 (2000) 27-32.
29. M.C. Jewett, J.R. Swartz, Substrate replenishment extends protein synthesis with an in vitro translation system designed to mimic the cytoplasm, Biotechnol. Bioeng. 87 (2004) 465-472.
30. T. Yamane, Y. Ikeda, T. Nagasaka, H. Nakano, Enhanced cell-free protein synthesis using a S30 extract from Escherichia coli grown rapidly at 42°C in an amino acid enriched medium, Biotechnol. Prog. 21 (2005) 608-613.
31. T. Kigawa, T. Yabuki, Y. Yoshida, M. Tsutsui, Y. Ito, T. Shibata, S. Yokoyama, Cell-free production and stable-isotope labeling of milligram quantities of proteins, FEBS Lett. 442 (1999) 15-19.