Conformational dynamics of estrogen receptors α and β as revealed by intrinsic tryptophan fluorescence and circular dichroism

Journal of Molecular Endocrinology

Volumn 35, Issue 2, 2005, Pages 211-223

  Nair, S K ^a   Thomas, T J ^a   Greenfield, N J ^a   Chen, A ^b   He, H ^b   Thomas, T ^a  

^a ROBERT WOOD JOHNSON MEDICAL SCHOOL   (United States)

^b RUTGERS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ESTRADIOL; ESTROGEN RECEPTOR ALPHA; ESTROGEN RECEPTOR BETA; TRYPTOPHAN;

ARTICLE; ATOMIC FORCE MICROSCOPY; CIRCULAR DICHROISM; CONTROLLED STUDY; ESTROGEN RESPONSIVE ELEMENT; FLUORESCENCE ANALYSIS; GENETIC TRANSCRIPTION; HIGH TEMPERATURE PROCEDURES; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CONFORMATION; PROTEIN FUNCTION; PROTEIN STABILITY; PROTEIN STRUCTURE; RECEPTOR BINDING; STRUCTURE ANALYSIS;

ACRYLAMIDE; CIRCULAR DICHROISM; ESTRADIOL; ESTROGEN RECEPTOR ALPHA; ESTROGEN RECEPTOR BETA; FEMALE; HUMANS; MALE; MICROSCOPY, ATOMIC FORCE; PARTICLE SIZE; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN ISOFORMS; RECOMBINANT PROTEINS; TRYPTOPHAN;

EID: 27644540697     PISSN: 09525041     EISSN: None     Source Type: Journal    
DOI: 10.1677/jme.1.01830     Document Type: Article

References (59)

1. Bouter A, Le Tilly V & Sire O 2005 Interplay of flexibility and stability in the control of estrogen receptor activity. Biochemistry 44 790-798.
2. Brooks SC & Skafar DF 2004 From ligand structure to biological activity: modified estratrienes and their estrogenic and antiestrogenic effects in MCF-7 cells. Steroids 69 401-418.
3. Brzozowski AM, Pike AC, Dauter Z, Hubbard RE, Bonn T, Engstrom O, Ohman L, Greene GL, Gustafsson JA & Carlquist M 1997 Molecular basis of agonism and antagonism in the oestrogen receptor. Nature 389 753-758.
4. Carlson KE, Choi I, Gee A, Katzenellenbogen BS & Katzenellenbogen JA 1997 Altered ligand binding properties and enhanced stability of a constitutively active estrogen receptor: evidence that an open pocket conformation is required for ligand interaction. Biochemistry 36 14897-14905.
5. Castro-Fernandez C, Maya-Nunez G & Conn PM 2005 Beyond the signal sequence: protein routing in health and disease. Endocrine Reviews 26 479-503.
6. Couse JF, Lindzey J, Grandien K, Gustafsson J-Å & Korach KS 1997 Tissue distribution and quantitative analysis of estrogen receptor-alpha (ERalpha) and estrogen receptor-beta (ERbeta) messenger ribonucleic acid in the wild-type and ERalpha-knockout mouse. Endocrinology 138 4613-4621.
7. Danielian PS, White R, Lees JA & Parker MG 1992 Identification of a conserved region required for hormone dependent transcriptional activation by steroid hormone receptors. EMBO Journal 11 1025-1033.
8. Eftink MR & Ghiron CA 1976 Exposure of tryptophanyl residues in proteins. Quantitative determination by fluorescence quenching studies. Biochemistry 15 672-680.
9. Eftink MR & Ghiron CA 1981 Fluorescence quenching studies with proteins. Analytical Biochemistry 114 199-227.
10. Forster C, Kietz S, Hultenby K, Warner M & Gustafsson JA 2004 Characterization of the ERbeta-/- mouse heart. PNAS 101 14234-14239.
11. Gee AC & Katzenellenbogen JA 2001 Probing conformational changes in the estrogen receptor: evidence for a partially unfolded intermediate facilitating ligand binding and release. Molecular Endocrinology 15 421-428.
12. Greenfield N, Vijayanathan V, Thomas TJ, Gallo MA & Thomas T 2001 Increase in the stability and helical content of estrogen receptor alpha in the presence of the estrogen response element: analysis by circular dichroism spectroscopy. Biochemistry 40 6646-6652.
13. Gustafsson J 1999 Estrogen receptor beta-a new dimension in estrogen mechanism of action. Journal of Endocrinology 163 379-383.
14. Hall JM, Chang CY & McDonnell DP 2000 Development of peptide antagonists that target estrogen receptor beta-coactivator interactions. Molecular Endocrinology 14 2010-2023.
15. Kamagata K, Arai M & Kuwajima K 2004 Unification of the folding mechanisms of non-two-state and two-state proteins. Journal of Molecular Biology 339 951-965.
16. Katzenellenbogen BS & Frasor J 2004 Therapeutic targeting in the estrogen receptor hormonal pathway. Seminars in Oncology 31 28-38.
17. Klinge CM 2001 Estrogen receptor interaction with estrogen response elements. Nucleic Acids Research 29 2905-2919.
18. Kosinski-Collins MS & King J 2003 In vitro unfolding, refolding, and polymerization of human gammaD crystallin, a protein involved in cataract formation. Protein Science 12 480-490.
19. Krieg AJ, Krieg SA, Ahn BS & Shapiro DJ 2004 Interplay between estrogen response element sequence and ligands controls in vivo binding of estrogen receptor to regulated genes. Journal of Biological Chemistry 279 5025-5034.
20. Kuiper GGJM, Enmark E, Pelto-Huikko M, Nilsson S & Gustafsson J-Å 1996 Cloning of a novel receptor expressed in rat prostate and ovary. PNAS 93 5925-5930.
21. Kumar R & Thompson EB 2003 Transactivation functions of the N-terminal domains of nuclear hormone receptors: protein folding and coactivator interactions. Molecular Endocrinology 17 1-10.
22. Kumar V, Green S, Staub A & Chambon P 1986 Localisation of the oestradiol-binding and putative DNA-binding domains of the human oestrogen receptor. EMBO Journal 5 2231-2236.
23. Lehrer SS 1971 Solute perturbation of protein fluorescence: the quenching of the tryptophyl fluorescence of model compounds and of lysozyme by iodide ion. Biochemistry 10 3254-3263.
24. Lopez D, Sanchez MD, Shea-Eaton W & McLean MP 2002 Estrogen activates the high-density lipoprotein receptor gene via binding to estrogen response elements and interaction with sterol regulatory element binding protein-1A. Endocrinology 143 2155-2168.
25. Loven MA, Likhite VS, Choi I & Nardulli AM 2001 Estrogen response elements alter coactivator recruitment through allosteric modulation of estrogen receptor beta conformation. Journal of Biological Chemistry 276 45282-45288.
26. Maaroufi Y & Leclercq G 1994 Importance of A/B and C domains of the estrogen receptor for its adsorption to hydroxylapatite. Steroid Biochemistry and Molecular Biology 48 155-163.
27. Manas ES, Xu ZB, Unwalla RJ & Somers WS 2004 Understanding the selectivity of genistein for human estrogen receptor-beta using X-ray crystallography and computational methods. Structure (Cambridge) 12 2197-2207.
28. Margeat E, Bourdoncle A, Margueron R, Poujol N, Cavaillès V & Royer C 2003 Ligands differentially modulate the protein interactions of the human estrogen receptors alpha and beta. Journal of Molecular Biology 326 77-92.
29. Matthews J & Gustafsson JA 2003 Estrogen signaling: a subtle balance between ER alpha and ER beta. Molecular Intervention 3 281-292.
30. McDonnell DP (2005) The molecular pharmacology of estrogen receptor modulators: implications for the treatment of breast cancer. Clinical Cancer Research 11 871s-877s.
31. McKenna NJ & O'Malley BW 2002 Combinatorial control of gene expression by nuclear receptors and coregulators. Cell 108 465-474.
32. Metivier R, Stark A, Flouriot G, Hubner MR, Brand H, Penot G, Manu D, Denger S, Reid G, Kos M et al. 2002 A dynamic structural model for estrogen receptor-alpha activation by ligands, emphasizing the role of interactions between distant A and E domains. Molecular Cell 10 1019-1032.
33. Metzger D, Ali S, Bornert JM & Chambon P 1995 Characterization of the amino-terminal transcriptional activation function of the human estrogen receptor in animal and yeast cells. Journal of Biological Chemistry 270 9535-9542.
34. Mosselman S, Polman J & Dijkema R 1996 ER beta: identification and characterization of a novel human estrogen receptor. FEBS Letters 392 49-53.
35. Nettles KW, Sun J, Radek JT, Sheng S, Rodriguez AL, Katzenellenbogen JA, Katzenellenbogen BS & Greene GL 2004 Allosteric control of ligand selectivity between estrogen receptors alpha and beta: implications for other nuclear receptors. Molecular Cell 13 317-327.
36. Pabo CO & Sauer RT 1992 Transcription factors: structural families and principles of DNA recognition. Annual Review of Biochemistry 61 1053-1095.
37. Pace CN 1986 Determination and analysis of urea and guanidine hydrochloride denaturation curves. Methods in Enzymology 131 266-280.
38. Paruthiyil S, Parmar H, Kerekatte V, Cunha GR, Firestone GL & Leitman DC 2004 Estrogen receptor beta inhibits human breast cancer cell proliferation and tumor formation by causing a G2 cell cycle arrest. Cancer Research 64 423-428.
39. Perczel A, Hollosi M, Tusnady G & Fasman GD 1991 Convex constraint analysis: a natural deconvolution of circular dichroism curves of proteins. Protein Engineering 4 669-679.
40. Pike AC, Brzozowski AM, Hubbard RE, Bonn T, Thorsell AG, Engstrom O, Ljunggren J, Gustafsson JA & Carlquist M 1999 Structure of the ligand-binding domain of oestrogen receptor beta in the presence of a partial agonist and a full antagonist. EMBO Journal 18 4608-4618.
41. Ptitsyn OB 1998 Protein folding: nucleation and compact intermediates. Biochemistry (Moscow) 63 367-373.
42. Ramsey TL, Risinger KE, Jernigan SC, Mattingly KA & Klinge CM 2004 Estrogen receptor beta isoforms exhibit differences in ligand-activated transcriptional activity in an estrogen response element sequence-dependent manner. Endocrinology 145 149-160.
43. Savitsky A & Golay MJE 1964 Smoothing and differentiation of data by simplified least square procedures. Analytical Chemistry 36 1627-1639.
44. Spolar RS & Record MT 1994 Coupling of local folding to site-specific binding of proteins to DNA. Science 263 777-784.
45. Strom A, Hartman J, Foster JS, Kietz S, Wimalasena J & Gustafsson, JA 2004 Estrogen receptor beta inhibits 17 beta-estradiol-stimulated proliferation of the breast cancer cell line T47D. PNAS 101 1566-1571.
46. Thomas T, Kulkarni GD, Gallo MA, Greenfield N, Lewis JS, Shirahata A & Thomas TJ 1997 Effects of natural and synthetic polyamines on the conformation of an oligodeoxyribonucleotide with the estrogen response element. Nucleic Acids Research 25 2396-2402.
47. Thomas T, Gallo MA & Thomas TJ 2004 Estrogen receptors as targets for drug development for breast cancer, osteoporosis and cardiovascular diseases Current Cancer Drug Targets 4 483-499.
48. Tremblay GB, Tremblay A, Copeland NG, Gilbert DJ, Jenkins NA, Labrie F & Giguere V 1997 Cloning, chromosomal localization, and functional analysis of the murine estrogen receptor beta. Molecular Endocrinology 11 353-365.
49. Tsai HW, Katzenellenbogen JA, Katzenellenbogen BS & Shupnik MA 2004 Protein kinase A activation of estrogen receptor alpha transcription does not require proteasome activity and protects the receptor from ligand-mediated degradation. Endocrinology 145 2730-2738.
50. Turgeon JL, McDonnell DP, Martin KA & Wise PM 2004 Hormone therapy: physiological complexity belies therapeutic simplicity. Science 304 1269-1273.
51. Uversky VN & Fink AL 2004 Conformational constraints for amyloid fibrillation: the importance of being unfolded. Biochimica et Biophysica Acta 1698 131-153.
52. van de Stolpe A, Slycke AJ, Reinders MO, Zomer AW, Goodenough S, Behl C, Seasholtz AF & van der Saag PT 2004 Estrogen receptor (ER)-mediated transcriptional regulation of the human corticotropin-releasing hormone-binding protein promoter: differential effects of ERalpha and ERbeta. Molecular Endocrinology 18 2908-2923.
53. Wacker JL, Zareie MH, Fong H, Sarikaya M & Muchowski PJ 2004 Hsp70 and Hsp40 attenuate formation of spherical and annular polyglutamine oligomers by partitioning monomer. Nature Structural & Molecular Biology 11 1215-1222.
54. Warnmark A, Almlof T, Leers J, Gustafsson JA & Treuter E 2001a Differential recruitment of the mammalian mediator subunit TRAP220 by estrogen receptors ERalpha and ERbeta. Journal of Biological Chemistry 276 23397-23404.
55. Warnmark A, Wikstrom A, Wright AP, Gustafsson JA & Hard T 2001b The N-terminal regions of estrogen receptor alpha and beta are unstructured in vitro and show different TBP binding properties. Journal of Biological Chemistry 276 45939-45944.
56. Webb P, Valentine C, Nguyen P, Price Jr RH, Marimuthu A, West BL, Baxter JD & Kushner PJ. 2003 ERbeta Binds N-CoR in the Presence of Estrogens via an LXXLL-like Motif in the N-CoR C-terminus. Nuclear Receptor 1 1336-1378.
57. Wood JR, Likhite VS, Loven MA & Nardulli AM 2001 Allosteric modulation of estrogen receptor conformation by different estrogen response elements. Molecular Endocrinology 15 1114-1126.
58. Yi P, Driscoll MD, Huang J, Bhagat S, Hilf R, Bambara RA & Muyan M 2002 The effects of estrogen-responsive element- and ligand-induced structural changes on the recruitment of cofactors and transcriptional responses by ER alpha and ER beta. Molecular Endocrinology 16 674-693.
59. Yon JM 2002 Protein folding in the post-genomic era. Journal of Cellular and Molecular Medicine 6 307-327.