Structure of the ligand-binding domain of oestrogen receptor beta in the presence of a partial agonist and a full antagonist

EMBO Journal

Volumn 18, Issue 17, 1999, Pages 4608-4618

  Pike, Ashley C W ^a   Brzozowski, Andrzej M ^a   Hubbard, Roderick E ^a   Bonn, Tomas ^b   Thorsell, Ann Gerd ^b   Engström, Owe ^b   Ljunggren, Jan ^b   Gustafsson, Jan Åke ^c   Carlquist, Mats ^a,b  

^a UNIVERSITY OF YORK   (United Kingdom)

^b Karo Bio   (Sweden)

^c KAROLINSKA INSTITUTE   (Sweden)

Author keywords

Activation function 2; Antagonist; Crystal structure; Oestrogen receptor; Phyto oestrogen

Indexed keywords

ESTRADIOL; ESTROGEN RECEPTOR; GENISTEIN; PARTIAL AGONIST; PHYTOHORMONE; RALOXIFENE; RECEPTOR SUBTYPE;

AMINO ACID SEQUENCE; ARTICLE; CHEMICAL STRUCTURE; CONTROLLED STUDY; DRUG RECEPTOR BINDING; ESCHERICHIA COLI; HORMONE BINDING; HYDROPHOBICITY; LIGAND BINDING; NONHUMAN; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; TRANSACTIVATION;

AMINO ACID SEQUENCE; ANIMALS; CRYSTALLOGRAPHY, X-RAY; ESTROGEN RECEPTOR BETA; GENISTEIN; HUMANS; LIGANDS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; RALOXIFENE; RATS; RECEPTORS, ESTROGEN; SEQUENCE HOMOLOGY, AMINO ACID; TAMOXIFEN;

EID: 0033198735     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/18.17.4608     Document Type: Article

References (43)

1. Adlercreutz, H. and Mazur, W. (1997) Phyto-oestrogens and Western diseases. Anal. Med., 29, 95-120.
2. Anstead, G.M., Carlson, K.E. and Katzenellenbogen, J.A. (1997) The estradiol pharmacophore: ligand structure-estrogen receptor binding affinity relationships and a model for the receptor binding site. Steroids, 62, 268-303.
3. Barkhem, T., Carlsson, B., Nilsson, Y., Enmark, E., Gustafsson, J.-Å. and Nilsson, S. (1998) Differential response of estrogen receptor α and estrogen receptor β to partial estrogen agonists/antagonists. Mol. Pharmacol., 54, 105-112.
4. Barton, G.J. (1993) ALSCRIPT: a tool to format multiple sequence alignments. Protein Eng., 6, 37-40.
5. Beato, M. and Sanchez-Pacheco, A. (1996) Interaction of steroid hormone receptors with the transcription initiation complex. Endocr. Rev., 17, 587-609.
6. Bourguet, W., Ruff, M., Chambon, P., Gronemeyer, H. and Moras, D. (1995) Crystal-structure of the ligand-binding domain of the human nuclear receptor RXR-α. Nature, 375, 377-382.
7. Brünger, A. (1992) X-PLOR Version 3.1: A System for X-ray Crystallography and NMR. Yale University Press, New Haven, CT.
8. Brzozowski, A.M. et al. (1997) Molecular basis of agonism and antagonism in the oestrogen receptor. Nature, 389, 753-758.
9. Collaborative Computational Project No. 4. (1994) The CCP4 suite: programs for protein crystallography. Acta Crystallogr. D, 50, 760-763.
10. Danielian, P.S., White, R., Lees, J.A. and Parker, M.G. (1992) Identification of a conserved region required for hormone dependent transcriptional activation by steroid hormone receptors. EMBO J., 11, 1025-1033.
11. Darimont, B.D., Wagner, R.L., Apriletti, J.W., Stallcup, M.R., Kushner, P.J., Baxter, J.D., Fletterick, R.J. and Yamamoto, K.R. (1998) Structure and specificity of nuclear receptor-coactivator interactions. Genes Dev., 12, 3343-3356.
12. Draper, M.W., Flowers, D.E., Huster, W.J., Neild, J.A., Harper, K.D. and Arnaud, C. (1996) A controlled trial of raloxifene (LY139481) HCl: impact on bone turnover and serum lipid profile in healthy postmenopausal women. J. Bone Miner. Res., 11, 835-842.
13. Esnouf, R.M. (1997) An extensively modified version of Molscript that includes greatly enhanced coloring capabilities. J. Mol. Graph., 15, 132-137.
14. Fawell, S.E., Lees, J.A., White, R. and Parker, M.G. (1990) Characterisation and colocalization of steroid binding and dimerisation activities in the mouse estrogen receptor. Cell, 60, 953-962.
15. Feng, W.J., Ribeiro, R.C.J., Wagner, R.L., Nguyen, H., Aprilleti, J.W., Fletterick, R.J., Baxter, J.D., Kushner, P.J. and West, B.L. (1998) Hormone-dependent coactivator binding to a hydrophobic cleft on nuclear receptors. Science, 280, 1747-1749.
16. Greene, G.L., Nolan, C., Engler, J.P. and Jensen, E.V. (1980) Monoclonal antibodies to the human estrogen receptor. Proc. Natl Acad. Sci. USA, 77, 5115-5119.
17. Grese, T.A. et al. (1997) Molecular determinants of tissue selectivity in estrogen receptor modulators. Proc. Natl Acad. Sci. USA, 94, 14105-14110.
18. Gustafsson, J.-Å. (1998) Raloxifene: magic bullet for heart and bone? Nature Med., 4, 152-153.
19. Jordan, V.C. (1998) Antiestrogenic action of raloxifene and tamoxifen: today and tomorrow. J. Natl Cancer Inst., 90, 967-971.
20. Katzenellenbogen, B.S. and Korach, K.S. (1997) A new actor in the estrogen receptor drama - enter ER-β. Endocrinology, 138, 861-862.
21. Klaholz, B.P., Renaud, J.-P., Mitschler, A., Zusi, C., Chambon, P., Gronemeyer, H. and Moras, D. (1998) Conformational adaptation of agonists to the human nuclear receptor RAR gamma. Nature Struct. Biol., 5, 199-202.
22. Kleywegt, G.J. and Jones, T.A. (1994) Detection, delineation, measurement and display of cavities in macromolecular structures. Acta Crystallogr. D, 50, 178-185.
23. Korach, K. (1994) Insights from the study of animals lacking functional estrogen receptor. Science, 266, 1524-1527.
24. Krege, J.H. et al. (1998) Generation and reproductive phenotypes of mice lacking estrogen receptor β. Proc. Natl Acad. Sci. USA, 95, 15677-15682.
25. Kuiper, G.G.J.M., Enmark, E., Pelto-Huikko, M., Nilsson, S. and Gustafsson, J.-Å. (1996) Cloning of a novel estrogen receptor expressed in rat prostate and ovary. Proc. Natl Acad. Sci. USA, 93, 5925-5930.
26. Kuiper, G.G.J.M., Carlsson, B., Grandien, J., Enmark, E., Häggblad, J., Nilsson, S. and Gustafsson, J.-Å. (1997) Comparison of the ligand binding specificity and transcript tissue distribution of the estrogen receptors α and β. Endocrinology, 138, 863-870.
27. Laskowski, R.A., MacArthur, M.W., Moss, D.S. and Thornton, J.M. (1993) PROCHECK - a program to check the stereochemical quality of protein structures. J. Appl. Crystallogr., 26, 283-291.
28. MacGregor, J.I. and Jordan, V.C. (1998) Basic guide to the mechanisms of antiestrogen action. Pharmacol. Rev., 50, 151-196.
29. McInerney, E.M., Weis, K.E., Sun, J., Mosselman, S. and Katzenellenbogen, B.S. (1998) Transcription activation by the human estrogen receptor subtype β (ERβ) studied with ERβ and ERα receptor chimeras. Endocrinology, 139, 4513-4522.
30. Moras, D. and Gronemeyer, H. (1998) The nuclear receptor ligand-binding domain: structure and function. Curr. Opin. Cell Biol., 10, 384-391.
31. Murshudov, G.N., Vagin, A.A. and Dodson, E.J. (1997) Refinement of macromolecular structures by the maximum-likelihood method. Acta Crystallogr. D, 53, 240-255.
32. Nolte, R.T. et al. (1998) Ligand-binding and co-activator assembly of the peroxisome proliferator-activated receptor-γ. Nature, 395, 137-143.
33. Ogawa, S., Inoue, S., Watanabe, T., Hiroi, H., Orimo, A., Hosoi, T., Ouchi, Y. and Muramatsu, M. (1998) The complete primary structure of human estrogen receptor β (hERβ) and its heterodimerisation with ERα in vivo and in vitro. Biochem. Biophys. Res. Commun., 243, 122-126.
34. Otwinowski, Z. and Minor, W. (1997) Processing X-ray diffraction data collected in oscillation mode. Methods Enzymol, 276, 307-326.
35. Paech, K., Webb, P., Kuiper, G.G.J.M., Nilsson, S., Gustafsson, J.-Å., Kushner, P.J. and Scanlan, T.S. (1997) Differential ligand activation of estrogen receptors ERα and ERβ at API sites. Science, 277, 1508-1510.
36. Renaud, J.P., Rochel, N., Ruff, M., Vivat, V., Chambon, P., Gronemeyer, H. and Moras, D. (1995) Crystal structure of the RAR-γ ligand-binding domain bound to all-trans retinoic acid. Nature, 378, 681-689.
37. Shiau, A.K., Barstad, D., Loria, P.M., Cheng, L., Kushner, P.J., Agard, D.A. and Greene, G.L. (1998) The structural basis of estrogen receptor/ coactivator recognition and the antagonism of this interaction by tamoxifen. Cell, 95, 927-937.
38. Stickle, D.F., Presta, L.G., Dill, K.A. and Rose, G.D. (1992) Hydrogen bonding in globular proteins. J. Mol. Biol., 226, 1143-1159.
39. Tanenbaum, D.M., Wang, Y., Williams, S.P. and Sigler, P.B. (1998) Crystallographic comparison of the estrogen and progesterone receptor's ligand binding domains. Proc. Natl Acad. Sci. USA, 95, 5998-6003.
40. Torchia, J., Glass, C.K. and Rosenfeld, M.G. (1998) Co-activators and co-repressors in the integration of transcriptional responses. Curr. Opin. Cell Biol., 10, 373-383.
41. Tsai, M.-J. and O'Malley, B.W. (1994) Molecular mechanisms of action of steroid/thyroid receptor superfamily members. Annu. Rev. Biochem., 63, 451-486.
42. Wagner, R.L., Apriletti, J.W., McGrath, M.E., West, B.L., Baxter, J.D. and Fletterick, R.J. (1995) A structural role for hormone in the thyroid hormone receptor. Nature, 378, 690-697.
43. Wurtz, J.-M., Bourguet, W., Renaud, J.-P., Vivat, V., Chambon, P., Moras, D. and Gronemeyer, H. (1996) A canonical structure for the ligand-binding domain of nuclear receptors. Nature Struct. Biol., 3, 87-94.