A Cdc42 mutant specifically activated by intersectin

Biochemistry

Volumn 44, Issue 40, 2005, Pages 13282-13290

  Smith, William J ^a,c   Hamel, Brant ^b   Yohe, Marielle E ^b   Sondek, John ^b   Cerione, Richard A ^a   Snyder, Jason T ^b  

^a Cornell University ^*  (United States)

^b UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^c NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; CELL CULTURE; COMPUTER SIMULATION; DATA ACQUISITION; ENZYME KINETICS;

BINDING ARCHITECTURE; CELL POLARITY; GUANINE NUCLEOTIDE EXCHANGE FACTORS; VESICULAR TRAFFICKING;

MUTAGENS;

ANTIPORTER; CELL PROTEIN; GUANOSINE TRIPHOSPHATASE; HYDROXYL GROUP; INTERSECTIN; PROTEIN CDC42; PROTEIN DBL; PROTEIN DBS; RHO GUANINE NUCLEOTIDE BINDING PROTEIN; TYROSINE; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; CATALYSIS; CELL POLARITY; CELL TRANSPORT; CYTOSKELETON; ENZYME ACTIVATION; HUMAN; HUMAN CELL; MOLECULAR RECOGNITION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN FAMILY; PROTEIN PROTEIN INTERACTION; SIGNAL TRANSDUCTION; STRUCTURE ANALYSIS;

ADAPTOR PROTEINS, VESICULAR TRANSPORT; AMINO ACID SEQUENCE; ANIMALS; CATALYSIS; CDC42 GTP-BINDING PROTEIN; CRYSTALLOGRAPHY, X-RAY; CYTOSKELETON; DNA, COMPLEMENTARY; GREEN FLUORESCENT PROTEINS; GUANINE; GUANINE NUCLEOTIDE EXCHANGE FACTORS; GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATE; HELA CELLS; HUMANS; KINETICS; MICE; MICROSCOPY, FLUORESCENCE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; PLASMIDS; POINT MUTATION; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; PROTO-ONCOGENE PROTEINS C-VAV; RHO GTP-BINDING PROTEINS; SIGNAL TRANSDUCTION; SURFACE PLASMON RESONANCE; TIME FACTORS; TYROSINE;

EID: 26444489047     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi050591b     Document Type: Article

References (45)

1. Erickson, J. W., and Cerione, R. A. (2001) Multiple roles for Cdc42 in cell regulation, Curr. Opin. Cell Biol. 13, 153-157.
2. Nobes, C. D., and Hall, A. (1999) Rho GTPases control polarity, protrusion, and adhesion during cell movement, J. Cell Biol. 144, 1235-1244.
3. Hall, A. (1999) Signal transduction pathways regulated by the Rho family of small GTPases, Br. J. Cancer 80, 25-27.
4. Hall, A. (1998) Rho GTPases and the actin cytoskeleton, Science 279, 509-514.
5. Malliri, A., and Collard, J. G. (2003) Role of Rho-family proteins in cell adhesion and cancer, Curr. Opin. Cell Biol. 15, 583-589.
6. Michiels, F., and Collard, J. G. (1999) Rho-like GTPases: Their role in cell adhesion and invasion, Biochem. Soc. Symp. 65, 125-146.
7. Overbeck, A. F., Brtva, T. R., Cox, A. D., Graham, S. M., Huff, S. Y., Khosravi-Far, R., Quilliam, L. A., Solski, P. A., and Der, C. J. (1995) Guanine nucleotide exchange factors: Activators of Ras superfamily proteins, Mol. Reprod. Dev. 42, 468-476.
8. Malliri, A., van der Kammen, R. A., Clark, K., van der Valk, M., Michiels, F., and Collard, J. G. (2002) Mice deficient in the Rac activator Tiam1 are resistant to Ras-induced skin tumours, Nature 417, 867-871.
9. Lin, R., Cerione, R. A., and Manor, D. (1999) Specific contributions of the small GTPases Rho, Rac, and Cdc42 to Dbl transformation, J. Biol Chem. 274, 23633-23641.
10. Cerione, R. A. (2004) Cdc42: New roads to travel, Trends Cell Biol. 14, 127-132.
11. Rossman, K. L., Der, C. J., and Sondek, J. (2005) GEF means go: Turning on RHO GTPases with guanine nucleotide-exchange factors, Nat. Rev. Mol. Cell Biol. 6, 167-180.
12. Erickson, J. W., and Cerione, R. A. (2004) Structural elements, mechanism, and evolutionary convergence of Rho protein-guanine nucleotide exchange factor complexes, Biochemistry 43, 837-842.
13. Abe, K., Rossman, K. L., Liu, B., Ritola, K. D., Chiang, D., Campbell, S. L., Burridge, K., and Der, C. J. (2000) Vav2 is an activator of Cdc42, Rac1, and RhoA, J. Biol. Chem. 275, 10141-10149.
14. Hart, M. J., Eva, A., Evans, T., Aaronson, S. A., and Cerione, R. A. (1991) Catalysis of guanine nucleotide exchange on the CDC42Hs protein by the dbl oncogene product, Nature 354, 311-314.
15. Wang, Y., Suzuki, H., Yokoo, T., Tada-Iida, K., Kihara, R., Miura, M., Watanabe, K., Sone, H., Shimano, H., Toyoshima, H., and Yamada, N. (2004) WGEF is a novel RhoGEF expressed in intestine, liver, heart, and kidney, Biochem. Biophys. Res. Commun. 324, 1053-1058.
16. Hussain, N. K., Jenna, S., Glogauer, M., Quinn, C. C., Wasiak, S., Guipponi, M., Antonarakis, S. E., Kay, B. K., Stossel, T. P., Lamarche-Vane, N., and McPherson, P. S. (2001) Endocytic protein intersectin-1 regulates actin assembly via Cdc42 and N-WASP, Nat. Cell Biol. 3, 927-932.
17. Mohney, R. P., Das, M., Bivona, T. G., Hanes, R., Adams, A. G., Philips, M. R., and O'Bryan, J. P. (2003) Intersectin activates Ras but stimulates transcription through an independent pathway involving JNK, J. Biol. Chem. 278, 47038-47045.
18. Predescu, S. A., Predescu, D. N., Timblin, B. K., Stan, R. V., and Malik, A. B. (2003) Intersectin regulates fission and internalization of caveolae in endothelial cells, Mol. Biol. Cell 14, 4997-5010.
19. Worthylake, D. K., Rossman, K. L., and Sondek, J. (2000) Crystal structure of Rac1 in complex with the guanine nucleotide exchange region of Tiaml, Nature 408, 682-688.
20. Rossman, K. L., Worthylake, D. K., Snyder, J. T., Siderovski, D. P., Campbell, S. L., and Sondek, J. (2002) A crystallographic view of interactions between Dbs and Cdc42: PH domain-assisted guanine nucleotide exchange, EMBO J. 21, 1315-1326.
21. Snyder, J. T., Worthylake, D. K., Rossman, K. L., Betts, L., Pruitt, W. M., Siderovski, D. P., Der, C. J., and Sondek, J. (2002) Structural basis for the selective activation of Rho GTPases by DbI exchange factors, Nat. Struct. Biol. 9, 468-475.
22. Kristelly, R., Gao, G., and Tesmer, J. J. (2004) Structural determinants of RhoA binding and nucleotide exchange in leukemia-associated Rho guanine-nucleotide exchange factor, J. Biol. Chem. 279, 47352-47362.
23. 2+ sensitization pathway in smooth muscle, Structure 12, 1955-1965.
24. Rossman, K. L., Worthylake, D. K., Snyder, J. T., Cheng, L., Whitehead, I. P., and Sondek, J. (2002) Functional analysis of cdc42 residues required for guanine nucleotide exchange, J. Biol. Chem. 277, 50893-50898.
25. Vetter, I. R., and Wittinghofer, A. (2001) The guanine nucleotide-binding switch in three dimensions, Science 294, 1299-1304.
26. Gao, Y., Xing, J., Streuli, M., Leto, T. L., and Zheng, Y. (2001) Trp(56) of rac1 specifies interaction with a subset of guanine nucleotide exchange factors, J. Biol. Chem. 276, 47530-47541.
27. Li, R., and Zheng, Y. (1997) Residues of the Rho family GTPases Rho and Cdc42 that specify sensitivity to Dbl-like guanine nucleotide exchange factors, J. Biol. Chem. 272, 4671-4679.
28. Bagrodia, S., Taylor, S. J., Jordon, K. A., Van Aelst, L., and Cerione, R. A. (1998) A novel regulator of p21-activated kinases, J. Biol. Chem. 273, 23633-23636.
29. Snyder, J. T., Rossman, K. L., Baumeister, M. A., Pruitt, W. M., Siderovski, D. P., Der, C. J., Lemmon, M. A., and Sondek, J. (2001) Quantitative analysis of the effect of phosphoinositide interactions on the function of Dbl family proteins, J. Biol. Chem. 276, 45868-45875.
30. Karnoub, A. E., Worthylake, D. K., Rossman, K. L., Pruitt, W. M., Campbell, S. L., Sondek, J., and Der, C. J. (2001) Molecular basis for Rac1 recognition by guanine nucleotide exchange factors, Nat. Struct. Biol. 8, 1037-1041.
31. Rudolph, M. G., Weise, C., Mirold, S., Hillenbrand, B., Bader, B., Wittinghofer, A., and Hardt, W. D. (1999) Biochemical analysis of SopE from Salmonella typhimurium, a highly efficient guanosine nucleotide exchange factor for RhoGTPases, J. Biol. Chem. 274, 30501-30509.
32. Schlumberger, M. C., Friebel, A., Buchwald, G., Scheffzek, K., Wittinghofer, A., and Hardt, W. D. (2003) Amino acids of the bacterial toxin SopE involved in G nucleotide exchange on Cdc42, J. Biol. Chem. 278, 27149-27159.
33. Nishikimi, A., Meller, N., Uekawa, N., Isobe, K., Schwartz, M. A., and Maruyama, M. (2005) Zizimin2: A novel, DOCK180-related Cdc42 guanine nucleotide exchange factor expressed predominantly in lymphocytes, FEBS Lett. 579, 1039-1046.
34. Wittinghofer, F. (1992) Three-dimensional structure of p21H-ras and its implications, Semin. Cancer Biol. 3, 189-198.
35. Rudolph, M. G., Wittinghofer, A., and Vetter, I. R. (1999) Nucleotide binding to the G12V-mutant of Cdc42 investigated by X-ray diffraction and fluorescence spectroscopy: Two different nucleotide states in one crystal, Protein Sci. 8, 778-787.
36. Longenecker, K., Read, P., Lin, S. K., Somlyo, A. P., Nakamoto, R. K., and Derewenda. Z. S. (2003) Structure of a constitutively activated RhoA mutant (Q63L) at 1.55 Å resolution, Acta Crystallogr. D59, 876-880.
37. Cool, R. H., Schmidt, G., Lenzen, C. U., Prinz, H., Vogt, D., and Wittinghofer, A. (1999) The Ras mutant D119N is both dominant negative and activated, Mol. Cell. Biol. 19, 6297-6305.
38. Geyer, M., and Wittinghofer, A. (1997) GEFs, GAPs, GDIs and effectors: Taking a closer (3D) look at the regulation of Ras-related GTP-binding proteins, Curr. Opin. Struct. Biol. 7, 786-792.
39. Dvorsky, R., and Ahmadian, M. R. (2004) Always look on the bright site of Rho: Structural implications for a conserved intermolecular interface, EMBO Rep. 5, 1130-1136.
40. Cherfils, J., Menetrey, J., Le Bras, G., Janoueix-Lerosey, I., de Gunzburg, J., Garel, J. R., and Auzat, I. (1997) Crystal structures of the small G protein Rap2A in complex with its substrate GTP with GDP and with GTPγS, EMBO J. 16, 5582-5591.
41. Fidyk, N. J., and Cerione, R. A. (2002) Understanding the catalytic mechanism of GTPase-activating proteins: Demonstration of the importance of switch domain stabilization in the stimulation of GTP hydrolysis, Biochemistry 41, 15644-15653.
42. Akasaka, K., Tamada, M., Wang, F., Kariya, K., Shima, F., Kikuchi, A., Yamamoto, M., Shirouzu, M., Yokoyama, S., and Kataoka, T. (1996) Differential structural requirements for interaction of Ras protein with its distinct downstream effectors, J. Biol. Chem. 271, 5353-5360.
43. Gao, Y., Dickerson, J. B., Guo, F., Zheng, J., and Zheng, Y. (2004) Rational design and characterization of a Rac GTPase-specific small molecule inhibitor, Proc. Natl. Acad. Sci. U.S.A. 101, 7618-7623.
44. Cheng, L., Rossman, K. L., Mahon, G. M., Worthylake, D. K., Korus, M., Sondek, J., and Whitehead, I. P. (2002) RhoGEF specificity mutants implicate RhoA as a target for Dbs transforming activity, Mol. Cell. Biol. 22, 6895-6905.
45. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F., and Higgins, D. G. (1997) The CLUSTAL_X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools, Nucleic Acids Res. 25, 4876-4882.