RhoGEF specificity mutants implicate RhoA as a target for Dbs transforming activity

Molecular and Cellular Biology

Volumn 22, Issue 19, 2002, Pages 6895-6905

  Cheng, Li ^a   Rossman, Kent L ^a   Mahon, Gwendolyn M ^a   Worthylake, David K ^a   Korus, Malgorzata ^a   Sondek, John ^a   Whitehead, Ian P ^a  

^a RUTGERS NEW JERSEY MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GUANINE NUCLEOTIDE EXCHANGE FACTOR; GUANOSINE TRIPHOSPHATASE; PROTEIN CDC42; RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR; RHOA GUANINE NUCLEOTIDE BINDING PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BIOTRANSFORMATION; CELL STRAIN; CONTROLLED STUDY; CORRELATION ANALYSIS; CRYSTAL STRUCTURE; GENE MUTATION; MOUSE; MUTANT; NONHUMAN; PRIORITY JOURNAL; PROTEIN STRUCTURE; SIGNAL TRANSDUCTION;

3T3 CELLS; ANIMALS; CDC42 GTP-BINDING PROTEIN; CELL TRANSFORMATION, NEOPLASTIC; FIBROBLASTS; GUANINE NUCLEOTIDE EXCHANGE FACTORS; GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATE; MICE; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RHOA GTP-BINDING PROTEIN; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY; TRANSFECTION;

ANIMALIA;

EID: 0036786484     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.22.19.6895-6905.2002     Document Type: Article

References (45)

1. Vav2 is an activator of Cdc42, Rac1, and RhoA
2. Activation of RhoA and SAPK/JNK signalling pathways by the RhoA-specific exchange factor mNET1
3. Characterization and expression of the human rhoH12 gene product
4. Biological assays for Ras transformation
5. The multidomain protein Trio binds the LAR transmembrane tyrosine phosphatase, contains a protein kinase domain, and has separate rac-specific and rho-specific guanine nucleotide exchange factor domains
6. Trp(56) of rac1 specifies interaction with a subset of guanine nucleotide exchange factors
7. The Dbl-related protein, Lfc, localizes to microtubules and mediates the activation of Rac signaling pathways in cells
8. Lfc and Lsc oncoproteins represent two new guanine nucleotide exchange factors for the Rho GTP-binding protein
9. Lck regulates Vav activation of members of the Rho family of GTPases
10. Role of substrates and products of PI 3-kinase in regulating activation of Rac-related guanosine triphosphatases by Vav
11. Cellular transformation and guanine nucleotide exchange activity are catalyzed by a common domain on the db1 oncogene product
12. Ras-mediated transcription activation: Analysis by transient cotransfection assays
13. Inhibition of invasion of epithelial cells by Tiam1-Rac signaling
14. A novel oncogene, ost, encodes a guanine nucleotide exchange factor that potentially links Rho and Rac signaling pathways
15. Molecular basis for Rac1 recognition by guanine nucleotide exchange factors
16. Dbl and Vav mediate transformation via mitogen-activated protein kinase pathways that are distinct from those activated by oncogenic Ras
17. Activation of Rac1, RhoA, and mitogen-activated protein kinases is required for Ras transformation
18. GAPs for rho-related GTPases
19. Specific contributions of the small GTPases Rho, Rac, and Cdc42 to Dbl transformation
20. NMR structure and mutagenesis of the N-terminal Dbl homology domain of the nucleotide exchange factor Trio
21. The CDC42-specific inhibitor derived from ACK-1 blocks v-Ha-Ras-induced transformation
22. Cdc42 regulates anchorage-independent growth and is necessary for Ras transformation
23. An essential role for Rac in Ras transformation
24. A role for Rho in Ras transformation
25. Guanine nucleotide exchange factors: Activators of the Ras superfamily of proteins
26. A comparative analysis of the phosphoinositide binding specificity of pleckstrin homology domains
27. Identification and characterization of hPEM-2, a guanine nucleotide exchange factor specific for Cdc42
28. Rhotekin, a new putative target for Rho bearing homology to a serine/threonine kinase, PKN, and rhophilin in the rho-binding domain
29. A crystallographic view of interactions between Dbs and Cdc42: PH domain-assisted guanine nucleotide exchange
30. Engineering multiple properties of a protein by combinatorial mutagenesis
31. Rac downregulates Rho activity: Reciprocal balance between both GTPases determines cellular morphology and migratory behavior
32. Matrix-dependent Tiam1/Rac signaling in epithelial cells promotes either cell-cell adhesion or cell migration and is regulated by phosphatidylinositol 3-kinase
33. Isolation and characterization of murine vav2, a member of the vav family of proto-oncogenes
34. Quantitative analysis of the effect of phosphoinositide interactions on the function of Dbl family proteins
35. Structural basis for the selective activation of Rho GTPases by Dbl exchange factors
36. Rac regulation of transformation, gene expression, and actin organization by multiple, PAK-independent pathways
37. Retroviral transduction and oncogenic selection of a cDNA encoding Dbs, a homolog of the Dbl guanine nucleotide exchange factor
38. Expression cloning of lfc, a novel oncogene with structural similarities to guanine nucleotide exchange factors and to the regulatory region of protein kinase C
39. Dbl family proteins
40. Dependence of Dbl and Dbs transformation on MEK and NF-κB activation
41. Crystal structure of Racl in complex with the guanine nucleotide exchange region of Tiaml
42. Control of the yeast bud-site assembly GTPase Cdc42. Catalysis of guanine nucleotide exchange by Cdc24 and stimulation of GTPase activity by Bern3
43. The faciogenital dysplasia gene product FGD1 functions as a Cdc42Hs-specific guanine-nucleotide exchange factor
44. Direct involvement of the small GTP-binding protein Rho in lbc oncogene function
45. Mas oncogene signaling and transformation require the small GTP-binding protein Rac