Downregulation of Wnt signaling by increased expression of Dickkopf-1 and -2 is a prerequisite for late-stage osteoblast differentiation of KS483 cells

Journal of Bone and Mineral Research

Volumn 20, Issue 10, 2005, Pages 1867-1877

  Van Der Horst, Geertje ^a   Van Der Werf, Steffie M ^a   Farih Sips, Hetty ^a   Van Bezooijen, Rutger L ^a   Löwik, Clemens W G M ^a   Karperien, Marcel ^a  

^a LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Dickkopf; Mineralization; Osteoblast; RNA interference; Wnt signaling

Indexed keywords

ALKALINE PHOSPHATASE; BETA CATENIN; BONE MORPHOGENETIC PROTEIN; DNA; FRIZZLED PROTEIN; LITHIUM CHLORIDE; LOW DENSITY LIPOPROTEIN RECEPTOR RELATED PROTEIN 5; PROTEIN; PROTEIN DICKKOPF 1; PROTEIN DICKKOPF 2; RNA; UNCLASSIFIED DRUG; WNT PROTEIN; WNT3 PROTEIN;

ANIMAL CELL; ARTICLE; BONE DEVELOPMENT; BONE MATRIX; BONE MINERALIZATION; CELL DIFFERENTIATION; CELL PROLIFERATION; CONTROLLED STUDY; DOWN REGULATION; HEMATOPOIETIC STEM CELL; IMMUNOFLUORESCENCE; MESENCHYMAL STEM CELL; MOUSE; NONHUMAN; OSTEOBLAST; PROTEIN LOCALIZATION; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA INTERFERENCE;

ACTIVE TRANSPORT, CELL NUCLEUS; ANIMALS; ANTIMANIC AGENTS; BETA CATENIN; BONE MARROW CELLS; CELL DIFFERENTIATION; CELL LINE; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; LITHIUM CHLORIDE; MALE; MESENCHYMAL STEM CELLS; MICE; OSTEOBLASTS; PROTEINS; SIGNAL TRANSDUCTION; UP-REGULATION; WNT PROTEINS;

EID: 25444433584     PISSN: 08840431     EISSN: None     Source Type: Journal    
DOI: 10.1359/JBMR.050614     Document Type: Article

References (35)

1. Gong Y, Slee RB, Fukai N, Rawadi G, Roman-Roman S, Reginato AM, Wang H, Cundy T, Glorieux FH, Lev D, Zacharin M, Oexle K, Marcelino J, Suwairi W, Heeger S, Sabatakos G, Apte S, Adkins WN, Allgrove J, Arslan-Kirchner M, Batch JA, Beighton P, Black GC, Boles RG, Boon LM, Borrone C, Brunner HG, Carle GF, Dallapiccola B, De Paepe A, Floege B, Halfhide ML, Hall B, Hennekam RC, Hirose T, Jans A, Juppner H, Kim CA, Keppler-Noreuil K, Kohlschuetter A, LaCombe D, Lambert M, Lemyre E, Letteboer T, Peltonen L, Ramesar RS, Romanengo M, Somer H, Steichen-Gersdorf E, Steinmann B, Sullivan B, Superti-Furga A, Swoboda W, van den Boogaard MJ, Van Hul W, Vikkula M, Votruba M, Zabel B, Garcia T, Baron R, Olsen BR, Warman ML 2001 LDL receptor-related protein 5 (LRP5) affects bone accrual and eye development. Cell 107:513-523.
2. Kato M, Patel MS, Levasseur R, Lobov I, Chang BH, Glass DA, Hartmann C, Li L, Hwang TH, Brayton CF, Lang RA, Karsenty G, Chan L 2002 Cbfa1-independent decrease in osteoblast proliferation, osteopenia, and persistent embryonic eye vascularization in mice deficient in Lrp5, a Wnt coreceptor. J Cell Biol 157:303-314.
3. Novak A, Dedhar S 1999 Signaling through beta-catenin and Lef/Tcf. Cell Mol Life Sci 56:523-537.
4. Wodarz A, Nusse R 1998 Mechanisms of Wnt signaling in development. Annu Rev Cell Dev Biol 14:59-88.
5. Veeman MT, Axelrod JD, Moon RT 2003 A second canon. Functions and mechanisms of beta-catenin-independent Wnt signaling. Dev Cell 5:367-377.
6. Lev D, Binson I, Foldes AJ, Watemberg N, Lerman-Sagie T 2003 Decreased bone density in carriers and patients of an Israeli family with the osteoporosis-pseudoglioma syndrome. Isr Med Assoc J 5:419-421.
7. Babij P, Zhao W, Small C, Kharode Y, Yaworsky PJ, Bouxsein ML, Reddy PS, Bodine PV, Robinson JA, Bhat B, Marzolf J, Moran RA, Bex F 2003 High bone mass in mice expressing a mutant LRP5 gene. J Bone Miner Res 18:960-974.
8. Boyden LM, Mao J, Belsky J, Mitzner L, Farhi A, Mitnick MA, Wu D, Insogna K, Lifton RP 2002 High bone density due to a mutation in LDL-receptor-related protein 5. N Engl J Med 346:1513-1521.
9. Little RD, Carulli JP, Del Mastro RG, Dupuis J, Osborne M, Folz C, Manning SP, Swain PM, Zhao SC, Eustace B, Lappe MM, Spitzer L, Zweier S, Braunschweiger K, Benchekroun Y, Hu X, Adair R, Chee L, FitzGerald MG, Tulig C, Caruso A, Tzellas N, Bawa A, Franklin B, McGuire S, Nogues X, Gong G, Allen KM, Anisowicz A, Morales AJ, Lomedico PT, Recker SM, Van Eerdewegh P, Recker RR, Johnson ML 2002 A mutation in the LDL receptor-related protein 5 gene results in the autosomal dominant high-bone-mass trait. Am J Hum Genet 70:11-19.
10. Van Wesenbeeck L, Cleiren E, Gram J, Beals RK, Benichou O, Scopelliti D, Key L, Renton T, Bartels C, Gong Y, Warman ML, De Vernejoul MC, Bollerslev J, Van Hul W 2003 Six novel missense mutations in the LDL receptor-related protein 5 (LRP5) gene in different conditions with an increased bone density. Am J Hum Genet 72:763-771.
11. Bain G, Muller T, Wang X, Papkoff J 2003 Activated beta-catenin induces osteoblast differentiation of C3H10T1/2 cells and participates in BMP2 mediated signal transduction. Biochem Biophys Res Commun 301:84-91.
12. Rawadi G, Vayssiere B, Dunn F, Baron R, Roman-Roman S 2003 BMP-2 Controls Alkaline Phosphatase Expression and Osteoblast Mineralization by a Wnt Autocrine Loop. J Bone Miner Res 18:1842-1853.
13. Bergwitz C, Wendlandt T, Kispert A, Brabant G 2001 Wnt's differentially regulate colony growth and differentiation of chondrogenic rat calvaria cells. Biochim Biophys Acta 1538:129-140.
14. Tian E, Zhan F, Walker R, Rasmussen E, Ma Y, Barlogie B, Shaughnessy JD Jr 2003 The role of the Wnt-signaling antagonist DKK1 in the development of osteolytic lesions in multiple myeloma. N Engl J Med 349:2483-2494.
15. Kahler RA, Westendorf JJ 2003 Lymphoid enhancer factor-1 and beta-catenin inhibit Runx2-dependent transcriptional activation of the osteocalcin promoter. J Biol Chem 278:11937-11944.
16. Boland GM, Perkins G, Hall DJ, Tuan RS 2004 Wnt 3a promotes proliferation and suppresses osteogenic differentiation of adult human mesenchymal stem cells. J Cell Biochem 93:1210-1230.
17. De Boer J, Wang HJ, Van Blitterswijk C 2004 Effects of Wnt signaling on proliferation and differentiation of human mesenchymal stem cells. Tissue Eng 10:393-401.
18. De Boer J, Siddappa R, Gaspar C, van Apeldoorn A, Fodde R, Van Blitterswijk C 2004 Wnt signaling inhibits osteogenic differentiation of human mesenchymal stem cells. Bone 34:818-826.
19. van der Horst G, Van Bezooijen RL, Deckers MM, Hoogendam J, Visser A, Lowik CWGM, Karperien M 2002 Differentiation of murine preosteoblastic KS483 cells depends on autocrine bone morphogenetic protein signaling during all phases of osteoblast formation. Bone 31:661-669.
20. van der Horst G, Farih-Sips H, Lowik CWGM, Karperien M 2003 Hedgehog stimulates only osteoblastic differentiation of undifferentiated KS483 cells. Bone 33:899-910.
21. Brummelkamp TR, Bernards R, Agami R 2002 A system for stable expression of short interfering RNAs in mammalian cells. Science 296:550-553.
22. He TC, Sparks AB, Rago C, Hermeking H, Zawel L, da Costa LT, Morin PJ, Vogelstein B, Kinzler KW 1998 Identification of c-MYC as a target of the APC pathway. Science 281:1509-1512.
23. Chomczynski P, Sacchi N 1987 Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156-159.
24. van Bezooijen RL, Farih-Sips H, Papapoulos SE, Lowik CWGM 1998 IL-1α, IL-1β, IL-6 and TNF-a steady state mRNA levels analyzed by reverse transcriptase competitive PCR in bone marrow of gonadectomized mice. J Bone Miner Res 13:185-194.
25. Livak KJ, Schmittgen TD 2001 Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25:402-408.
26. Olmeda D, Castel S, Vilaro S, Cano A 2003 Beta-catenin regulation during the cell cycle: Implications in G2/M and apoptosis. Mol Biol Cell 14:2844-2860.
27. Mao B, Niehrs C 2003 Kremen2 modulates Dickkopf2 activity during Wnt/LRP6 signaling. Gene 302:179-183.
28. Mao B, Wu W, Davidson G, Marhold J, Li M, Mechler BM, Delius H, Hoppe D, Stannek P, Walter C, Glinka A, Niehrs C 2002 Kremen proteins are Dickkopf receptors that regulate Wnt/beta-catenin signalling. Nature 417:664-667.
29. Vaes BL, Dechering KJ, Feijen A, Hendriks JM, Lefevre C, Mummery CL, Olijve W, van Zoelen EJ, Steegenga WT 2002 Comprehensive microarray analysis of bone morphogenetic protein 2-induced osteoblast differentiation resulting in the identification of novel markers for bone development. J Bone Miner Res 17:2106-2118.
30. Chung YS, Baylink DJ, Srivastava AK, Amaar Y, Tapia B, Kasukawa Y, Mohan S 2004 Effects of secreted frizzled-related protein 3 on osteoblasts in vitro. J Bone Miner Res 19:1395-1402.
31. Zechner D, Fujita Y, Hulsken J, Muller T, Walther I, Taketo MM, Crenshaw EB III, Birchmeier W, Birchmeier C 2003 Beta-catenin signals regulate cell growth and the balance between progenitor cell expansion and differentiation in the nervous system. Dev Biol 258:406-418.
32. Gottlieb D 2002 Signposts on the neural path. Nat Biotechnol 20:1208-1210.
33. Pinto D, Gregorieff A, Begthel H, Clevers H 2003 Canonical Wnt signals are essential for homeostasis of the intestinal epithelium. Genes Dev 17:1709-1713.
34. Sancho E, Batlle E, Clevers H 2003 Live and let die in the intestinal epithelium. Curr Opin Cell Biol 15:763-770.
35. van de Wetering M, Sancho E, Verweij C, de Lau W, Oving I, Hurlstone A, van der Horn K, Batlle E, Coudreuse D, Haramis AP, Tjon-Pon-Fong M, Moerer P, van den Born M, Soete G, Pals S, Eilers M, Medema R, Clevers H 2002 The beta-catenin/TCF-4 complex imposes a crypt progenitor phenotype on colorectal cancer cells. Cell 111:241-250.