Solution structure of BmKK2, a new potassium channel blocker from the venom of Chinese scorpion Buthus martensi Karsch

Proteins: Structure, Function and Genetics

Volumn 55, Issue 4, 2004, Pages 835-845

  Zhang, Naixia ^a   Li, Minghua ^b   Chen, Xiang ^a   Wang, Yuefeng ^a   Wu, Gong ^a   Hu, Guoyuan ^b   Wu, Houming ^a  

^a SHANGHAI INSTITUTE OF ORGANIC CHEMISTRY   (China)

^b SHANGHAI INSTITUTE OF MATERIA MEDICA   (China)

Author keywords

BmKK2; Buthus martensi Karsch; Kv channel; NMR solution structure; Potassium channel blocker; RSK channel; Scorpion toxin

Indexed keywords

CHARYBDOTOXIN; NOXIUSTOXIN; POTASSIUM CHANNEL; PROTEIN BMKK2; SCORPION VENOM; UNCLASSIFIED DRUG;

ALPHA HELIX; AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; BETA SHEET; BUTHUS MARTENSI; CHANNEL GATING; DRUG ISOLATION; DRUG MECHANISM; DRUG STRUCTURE; HIPPOCAMPUS; HYDROGEN BOND; MOLECULAR DYNAMICS; NONHUMAN; NUCLEAR OVERHAUSER EFFECT; PATCH CLAMP; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN SECONDARY STRUCTURE; RAT; SCORPION;

AMINO ACID SEQUENCE; ANIMALS; ELECTRIC CONDUCTIVITY; HYDROGEN BONDING; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NEURONS; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PATCH-CLAMP TECHNIQUES; POTASSIUM CHANNEL BLOCKERS; POTASSIUM CHANNELS; POTASSIUM CHANNELS, CALCIUM-ACTIVATED; PROTEIN STRUCTURE, SECONDARY; RATS; RATS, SPRAGUE-DAWLEY; SCORPION VENOMS; SEQUENCE ALIGNMENT; SMALL-CONDUCTANCE CALCIUM-ACTIVATED POTASSIUM CHANNELS; STRUCTURE-ACTIVITY RELATIONSHIP;

BUTHUS; MESOBUTHUS MARTENSII; SCORPIONES;

EID: 2542566500     PISSN: 08873585     EISSN: None     Source Type: Journal    
DOI: 10.1002/prot.20117     Document Type: Article

References (60)

1. Rochat H, Bernard P, Couraud F. Scorpion toxins: chemistry and mode of action. In: Ceccarelli B, Clementi F, editor. Advances in cytopharmacology. Vol 3B. New York: Raven Press; 1979. p 325-334.
2. Possani LD. In: Tu AT, editor. Handbook of natural toxins. Vol 2. New York: Marcel Dekker Inc.; 1984. p 513-550.
3. Zlotkin E, Eitan M, Bindokas VP, Adams ME, Moyer M, Burkhart W, Fowler E. Functional duality and structural uniqueness of depressant insect-selective neurotoxins. Biochemistry 1991;30:4814-4821.
4. Kopeyan C, Martinez G, Lissitzky S, Miranda F, Rochat H. Disulfide bonds of toxin II of the scorpion Androctonus australis Hector. Eur J Biochem 1974;47:483-489.
5. + channels by a novel scorpion toxin. Nature 1982;296:90-91.
6. Martin BM, Ramirez AN, Gurrola GB, Nobile M, Prestipino G, Possani LD. Novel K(+) channel-blocking toxins from the venom of the scorpion Centruroides limpidus limpidus Karsch. Biochem J 1994;304(Pt1):51-56.
7. + channels. Mol Pharmacol 1996;50:1167-1177.
8. + channel blocking toxin from the scorpion Pandinus imperator. Biochem J 1996;315:977-981.
9. Garcia ML, Gao Y-D, McManus OB, Kaczorowski GJ. Potassium channels: from scorpion venoms to high-resolution structure. Toxicon 2001;39:739-748.
10. Garcia-Calvo M, Knaus HG, McManus OB, Giangiacomo KM, Kaczorowski GJ, Garcia ML. Purification and reconstitution of the high-conductance, calcium-activated potassium channel from tracheal smooth muscle. J Biol Chem 1994;269:676-682.
11. Garcia ML, Hanner M, Knaus HG, Koch R, Schmalhofer W, Slaughter RS, Kaczorowski GJ. Pharmacology of potassium channels. Adv Pharmacol 1997;39:425-471.
12. + channel blocking peptide purified from the venom of the scorpion Centruroides noxius Hoffmann. Carlsberg Res Commun 1982;47:285-289.
13. Tytgat J, Chandy KG, Garcia ML, Gutman GA, Martin-Eauclaire MF, et al. A unified nomenclature for short-chain peptides isolated from scorpion venoms: alpha-KTx molecular subfamilies. Trends Pharmacol Sci 1999;20:444-447.
14. Goudet C, Chi C-W, Tytgat J. An overview of toxins and genes from the venom of the Asian scorpion Buthus martensi Karsch. Toxicon 2002;40:1239-1258.
15. Bontems F, Roumestand C, Boyot P, Gilquin B, Doljansky Y, Menez A, Toma F. Three-dimensional structure of natural charybdotoxin in aqueous solution by 1H-NMR. Charybdotoxin possesses a structural motif found in other scorpion toxins. Eur J Biochem 1991;196:19-28.
16. Dauplais M, Gilquin B, Possani LD, Gurrola-Briones G, Roumestand C, Menez A. Determination of the three-dimensional solution structure of noxiustoxin: analysis of structural differences with related short-chain scorpion toxins. Biochemistry 1995;34:16563-16573.
17. Blanc E, Romi-Lebrun R, Bornet O, Nakajima T, Darbon H. Solution structure of two new toxins from the venom of the Chinese scorpion Buthus martensi Karsch blockers of potassium channels. Biochemistry 1998;37:12412-12418.
18. Meunier S, Bernassau JM, Sabatier JM, Martin-Eauclaire MF, Van Rietschoten J, Cambillau C, Darbon H. Solution structure of P05-NH2, a scorpion toxin analog with high affinity for the apamin-sensitive potassium channel. Biochemistry 1993;32:11969-11976.
19. Fernandez I, Romi R, Szendeffy S, Martin-Eauclaire MF, Rochat H, Van Rietschoten J, Pons M, Giralt E. Kaliotoxin (1-37) shows structural differences with related potassium channel blockers. Biochemistry 1994;33:14256-14263.
20. Wang I, Wu S-H, Chang H-K, Shieh R-C, Yu H-M, Chen C. Solution structure of a K(+)-channel blocker from the scorpion Tityus cambridgei. Protein Sci 2002;11:390-400.
21. Wu G, Li Y, Wei D, He F, Jiang S, Hu G, Wu H. Solution structure of BmP01 from the venom of scorpion Buthus martensii Karsch. Biochem Biophys Res Commun 2000;276:1148-1154.
22. + channel-blocking peptide from venom of Chinese scorpion Buthus martensii Karsch. Acta Pharmacologica Sinica 1998;19:317-321.
23. Zeng XC, Peng F, Luo F, Zhu SY, Liu H, Li WX. Molecular cloning and characterization of four scorpion K(+)-toxin-like peptides: a new subfamily of venom peptides (alpha-KTx14) and genomic analysis of a member. Biochimie 2001;83:883-889.
24. Zhang J-M, Hu G-Y. Huperzine A, a nootropic alkaloid, inhibits N-methyl-D-aspartate-induced current in rat dissociated hippocampal neurons. Neuroscience 2001;105:663-669.
25. Klee R, Ficker E, Heinemann U. Comparison of voltage-dependent potassium currents in rat pyramidal neurons acutely isolated from hippocampal regions CA1 and CA3. J Neurophysiol 1995;74:1982-1995.
26. Wu HM, Wu G, Huang XL, He FH, Jiang SK. Purification, characterization and structural study of the neuro-peptides from scorpion Buthus martensi Karsch. Pure Appl Chem 1999;71:1157-1162.
27. Piantini U, Sorensen OW, Ernst RR. Multiple quantum filters for elucidating NMR coupling networks. J Am Chem Soc 1982;104:6800-6801.
28. Braunschweilier L, Ernst RR. Coherence transfer by isotropic mixing: application to proton correlation spectroscopy. J Magn Reson 1988;53:521-528.
29. Griesinger C, Otting G, Wüthrich K, Ernst RR. Clean TOCSY for proton spin system identification in macromolecules. J Am Chem Soc 1988;110:7870-7872.
30. Jeener J, Meier BH, Bachmann P, Ernst RR. Investigation of exchange process by two-dimensional NMR spectroscopy. J Chem Phys 1979;71:4546-4553.
31. Drobny G, Pines A, Sinton S, Weitekamp D, Wemmer D. Fourier transform multiple quantum nuclear magnetic resonance. Faraday Symp Chem Soc 1979;13:49-55.
32. Bax AD, Davis DG. MLEV-17-based two-dimensional homonuclear magnetization transfer spectroscopy. J Magn Reson 1985;65:355-366.
33. Bartels C, Xia T-H, Billeter M, Güntert P, Wüthrich K. The program XEASY for computer-supported NMR spectral analysis of biological macromolecules. J Biomol NMR 1995;6:1-10.
34. Wüthrich K. NMR of proteins and nucleic acid. New York: John Wiley & Sons; 1986. 292 p.
35. Güntert P, Mumenthaler C, Wüthrich K. Torsion angle dynamics for NMR structure calculation with the new program DYANA. J Mol Biol 1997;273:283-298.
36. Weiner SJ, Kollman PA. AMBER: assisted model building with energy refinement. A general program for modeling molecules and their interactions. J Comput Chem 1981;2:287-309.
37. Weiner SJ, Kollman PA, Nguyen DT, Case DA. An all-atom force field for simulations of proteins and nucleic acids. J Comput Chem 1986;2:230-252.
38. Laskowski RA, MacArthur MW, Moss DS, Thornton JM. PROCHECK: a program to check the stereochemical quality of protein structures. J Appl Crystallogr 1993;26:283-291.
39. Laskowski RA, Rullmann JAC, MacArthur MW, Kaptein R, Thornton JM. AQUA and PROCHECK-NMR: programs for checking the quality of protein structures solved by NMR. J Biomol NMR 1996;8:477-486.
40. Koradi R, Billeter M, Wüthrich K. MOLMOL: a program for display and analysis of macromolecular structures. J Mol Graph 1996;14:51-55.
41. Folander K, Douglass J, Swanson R. Confirmation of the assignment of the gene encoding Kv1.3, a voltage-gated potassium channel (KCNA3) to the proximal short arm of human chromosome 1. Genomics 1994;23:295-296.
42. Köhler M, Hirschberg B, Bond CT, Kinzie JM, Marrion NV, Maylie J, Adelman JP. Small-conductance, calcium-activated potassium channels from mammalian brain. Science 1996;273:1709-1714.
43. + conduction and selectivity. Science 1998;280:69-77.
44. Gao Y-D, Maria LG. Interaction of agitoxin2, charybdotoxin, and iberiotoxin with potassium channels: selectivity between voltage-gate and maxi-K channels Proteins 2003;52:146-154.
45. Jones TA, Kjeldgaard M. "Essential O" software manual. Uppsala University, Uppsala, Sweden; 1998.
46. Güntert P, Braun W, Wüthrich K. Efficient computation of three-dimensional protein structures in solution fromnuclear magnetic resonance data using the program DIANA and the supporting programs CALIBA, HABAS and GLOMSA. J Mol Biol 1991;217:517-530.
47. Güntert P, Qian YQ, Otting G, Muller M, Gehring WJ, Wüthrich K. Structure determination of the Antp (C39-S) homeodomain from nuclearmagnetic resonance data in solution using a novel strategy for the structure calculation with the programs DIANA, CALIBA, HABAS and GLOMSA. J Mol Biol 1991;217:531-540.
48. Bontems F, Roumestand C, Gilquin B, Toma F. Refined structure of charybdotoxin: common motifs in scorpion toxins and insect defensins. Science 1991;254:1521-1523.
49. Blanc E, Sabatier JM, Kharrat R, Meunier S, el Ayeb M, Van Rietschoten J, Darbon H. Solution structure of maurotoxin, a scorpion toxin from Scorpio maurus, with high affinity for voltage-gated potassium channels. Proteins 1997;29:321-33.
50. McDonald IK, Thornton JM. Satisfying hydrogen bonding potential in proteins. J Mol Biol 1994;238:777-793.
51. Wallace AC, Laskowski RA, Thornton JM. LIGPLOT: a program to generate schematic diagrams of protein-ligand interactions. Protein Eng 1995;8:127-134.
52. + channel structure from a complete functional map of the molecular surface of charybdotoxin. Biochemistry 1994;33:443-450.
53. Goldstein SAN, Pheasant DJ, Miller C. The charybdotoxin receptor of a Shaker K+ channel: peptide and channel residues mediating molecular recognition. Neuron 1994;12:1377-1388.
54. + channel. Neuron 1996;16:123-130.
55. + channel. Neuron 1992;9:307-313.
56. Pardo-Lopez L, Zhang M, Liu J, Jiang M, Possani LD, Tseng G-N. Mapping the binding site of a human ether-a-go-go-related gene-specific peptide toxin (ErgTx) to the channel's outer vestibule. J Biol Chem 2002;277:16403-16411.
57. Aiyar J, Rizzi JP, Gutman GA, Chandy KG. The signature sequence of voltage-gated potassium channels projects into the external vestibule. J Biol Chem 1996;271:31013-31016.
58. Gross A, MacKinnon R. Agitoxin footprinting the shaker potassium channel pore. Neuron 1996;16:399-406.
59. Cui M, Shen J-H, James MB, Fu W, Wu J-J, Zhang Y-M, Luo X-M, Chi Z-W, Ji R-Y, Jiang H-L, Chen K-X. J Mol Biol 2002;318:417-428.
60. + channel revealed by the NMR-derived structures of scorpion toxins. Neuron 1995;15:1169-1181.