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12
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0027161159
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A. Butler, S. Tsunoda, D. P. McCobb, A. Wei, L. Salkoff, Science 261, 221 (1993).
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(1993)
Science
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, pp. 221
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Butler, A.1
Tsunoda, S.2
McCobb, D.P.3
Wei, A.4
Salkoff, L.5
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18
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85035173702
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note
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vCa (mslo; L16912).
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19
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85035176777
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note
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Abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; Y, Tyr, and X, unknown.
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20
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85035171501
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note
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7 cpm/ml in hybridization buffer: 66% formamide, 260 mM NaCl, 1.3x Denhardt solution (13 mM tris, pH 8.0, 1.3 mM EDTA, 13% dextran sulfate). Sections in the hybridization mixture were covered with siliconized glass cover slips and sealed with DPX mountant. After the slides had been incubated at 58°C for 20 hours, they were soaked in 4x SSC to remove cover slips, then rinsed in 4x SSC (four times, 5 min each) before treatment with ribonuclease A (20 mg/ml for 30 min at 37°C). The slides were then rinsed in decreasing concentrations of SSC containing 1 mM dithiothreiotol (DTT) to a final stringency of 0.1 x SSC, 1 mM DTT for 30 min at 65°C. After the sections had been dehydrated in increasing concentrations of ethanol, they were vacuum-dried and exposed to DuPont Cronex-4 x-ray film for 7 days. The film was scanned by a Microtek ScanMaker 1850S at a resolution of 728 pixels per centimeter, and the images were analyzed with Image v1.55 software (NIH) and Photoshop (Adobe).
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22
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0026794812
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2, 5 mM Hepes (pH 7.5 with NaOH) at room temperature. To minimize Cl currents, some oocytes were soaked and studied in Cl -free ND96 solution (96 mM sodium gluconate, 2 mM potassium gluconate, 2.7 mM calcium gluconate, 1-mM magnesium gluconate, 5 mM Hepes, pH 7.5 with NaOH).
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(1992)
Neuron
, vol.9
, pp. 209
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Adelman, J.P.1
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23
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10144255322
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K. M. Houamed et al., Science 349, 760 (1991).
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(1991)
Science
, vol.349
, pp. 760
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Houamed, K.M.1
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24
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0026093312
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M. Masu, Y. Tanabe, K. Tsuchida, R. Shigemoto, S. Nakanishi. Nature 349, 760 (1991).
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(1991)
Nature
, vol.349
, pp. 760
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Masu, M.1
Tanabe, Y.2
Tsuchida, K.3
Shigemoto, R.4
Nakanishi, S.5
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25
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85035181528
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note
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After establishment of the two-electrode voltage clamp, the oocyte was impaled with a third electrode containing 200 mM EGTA, pH adjusted to 7.2 with KOH. We monitored the input resistance during impalement to ensure oocyte viability. At the indicated time, 50 nl of the EGTA solution was injected into the oocyte. If we assume an oocyte volume of 1 μl, the predicted final concentration of EGTA was 10 mM.
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26
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85035177441
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note
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+ current than that activated by glutamate in oocytes coinjected with mGluR1a and rSK2.
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28
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0018733531
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2+ (0.1 to 3 mM) were added to the intracellular face of inside-out patches, outward currents were reduced. Apamin was from Calbiochem, and d-tubocurare was from Research Biochemicals International.
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(1979)
J. Physiol. (London)
, vol.75
, pp. 463
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Fabiato, A.1
Fabiato, F.2
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29
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85035173891
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note
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2+.
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37
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0028998041
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D. M. Papazian et al., Neuron 14, 1293 (1995).
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(1995)
Neuron
, vol.14
, pp. 1293
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Papazian, D.M.1
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39
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85035177501
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note
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We thank M. Litt for assistance with database searches, J. Saugstad and G. Westbrook for early experiments with mGluR1a, and B. Bean for helpful and enthusiastic discussions. We thank G. Westbrook for supplying the mGluR1a clone and C. Miller for supplying the charybdotoxin. M.K. was supported by a grant from the DRG, B.H. by an NIH cardiology training grant, and J.M.K. by a National Research Service Award grant. This work was supported by NIH grants to J.M. and J.P.A
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