Structural biology: Structure of SARS coronavirus spike receptor-binding domain complexed with receptor

Science

Volumn 309, Issue 5742, 2005, Pages 1864-1868

  Li, Fang ^a   Li, Wenhui ^a   Farzan, Michael ^a   Harrison, Stephen C ^a,b  

^a HARVARD MEDICAL SCHOOL   (United States)

^b BOSTON CHILDREN S HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYMES; POLYPEPTIDES; STABILIZATION;

ANGIOTENSIN-CONVERTING ENZYME 2 (ACE2); CROSS-SPECIES INFECTION; PEPTIDASE; RECEPTOR-BINDING DOMAIN (RBD);

PROTEINS;

CORONA VIRUS VACCINE; DIPEPTIDYL CARBOXYPEPTIDASE; DIPEPTIDYL CARBOXYPEPTIDASE 2; PEPTIDASE; UNCLASSIFIED DRUG; VIRUS PROTEIN; VIRUS RECEPTOR; VIRUS SPIKE PROTEIN; VIRUS VACCINE; ANGIOTENSIN CONVERTING ENZYME 2; CARBOXYPEPTIDASE; EPITOPE; MEMBRANE PROTEIN; SPIKE GLYCOPROTEIN, CORONAVIRUS; VIRUS ANTIBODY; VIRUS ENVELOPE PROTEIN;

BIOLOGY;

ARTICLE; CRYSTAL STRUCTURE; DRUG DESIGN; HUMAN; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN VARIANT; RECEPTOR AFFINITY; RECEPTOR BINDING; SARS CORONAVIRUS; VIRUS TRANSMISSION; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMAL; BINDING SITE; CARNIVORA; CELL LINE; CHEMICAL STRUCTURE; CHEMISTRY; DISEASE TRANSMISSION; EPIDEMIC; GENETICS; GLYCOSYLATION; HYDROPHOBICITY; IMMUNOLOGY; METABOLISM; MOLECULAR GENETICS; MUTATION; PHYSIOLOGY; PROTEIN CONFORMATION; PROTEIN TERTIARY STRUCTURE; SEVERE ACUTE RESPIRATORY SYNDROME; SPECIES DIFFERENCE; VIROLOGY; X RAY CRYSTALLOGRAPHY;

CORONA VIRUS; CORONAVIRUS; SARS CORONAVIRUS;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; ANTIBODIES, VIRAL; BINDING SITES; CARBOXYPEPTIDASES; CELL LINE; CRYSTALLOGRAPHY, X-RAY; DISEASE OUTBREAKS; EPITOPES; GLYCOSYLATION; HUMANS; HYDROPHOBICITY; MEMBRANE GLYCOPROTEINS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; PEPTIDYL-DIPEPTIDASE A; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; RECEPTORS, VIRUS; SARS VIRUS; SEVERE ACUTE RESPIRATORY SYNDROME; SPECIES SPECIFICITY; VIRAL ENVELOPE PROTEINS; VIRAL VACCINES; VIVERRIDAE;

EID: 24944498409     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1116480     Document Type: Article

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36. Single-letter abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
37. We thank staff at the Advanced Light Source beam-lines 8.2.1 and 8.2.2 for assistance and M. Berardi and E. Settembre for discussions. This work was supported by NIH grants CA13202 (to S.C.H.) and AI061601 (to M.R.F.). S.C.H. is an Investigator in the Howard Hughes Medical Institute. Coordinates and structure factors have been submitted to the Protein Data Bank with accession number 2AJF.