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0031593021
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AQ-linked phosphoramidites were prepared by using 2-cyanoethyl-N,N- diisopropylchlorophosphoramidite. See: Gasper, S. M.; Schuster, G. B. J. Am. Chem. Soc. 1997, 119, 12762.
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24644494953
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note
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5′-Thiol modifier C6 S-S and 3′-thiol modifier C3 S-S CPG were from Glen Research. All DNAs synthesized were confirmed by MALDI-TOF MS.
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26
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24644521813
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note
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- calcd for SMe-capped 5′-SH-DNA and 3′-SH-DNA, 4494.8 and 3211.1, respectively; found 4495.0 and 3211.0.
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27
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24644468831
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note
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2 is inefficient.
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28
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6344249017
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(a) Nauser, T.; Felling, J.; Schoneich, C. Chem. Res. Toxicol. 2004, 17, 1323.
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31
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24644450920
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note
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2 facilitates the second oxidation step, that is, removal of an electron from the disulfide radical anion.
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34
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24644500977
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note
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For these interduplex controls, a mixture of duplexes, one containing AQ-modified duplex DNA (with no thiols) and the other DNA modified with two thiols but without AQ, was irradiated at the same concentrations and conditions as for the AQ-modified duplexes containing thiols. The AQ-modified DNA used has the sequence of DNA2, while the DNA lacking AQ has the sequence of DNA1.
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36
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0033772184
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(b) Boon, E. M.; Ceres, D. M.; Drummond, T. G.; Hill, M. G.; Barton, J. K. Nature Biotechnol. 2000, 18, 1096.
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37
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0037471638
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0033560675
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Marathias, V.M.1
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39
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24644514122
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note
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m is 65.4 °C.
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40
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0037035314
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Note that an increase in oxidation efficiency with DNA flexibility was also seen for a reaction from the sugar backbone. See: Giese, B.; Biland, A. Chem. Commun. 2002, 667.
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Giese, B.1
Biland, A.2
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