Alanine Scanning Mutagenesis of the Testosterone Binding Site of Rat 3α-Hydroxysteroid Dehydrogenase Demonstrates Contact Residues Influence the Rate-Determining Step

Biochemistry

Volumn 43, Issue 19, 2004, Pages 5832-5841

  Heredia, Vladi V ^a   Cooper, William C ^a   Kruger, Ryan G ^a   Jin, Yi ^a   Penning, Trevor M ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; ENZYME INHIBITION; ENZYMES; FLUORESCENCE; HYDROPHOBICITY; MUTAGENESIS; RATE CONSTANTS; TITRATION;

CATALYTIC RESIDUES; COMPETITIVE INHIBITORS; MUTATION; TESTOSTERONE BINDING SITES;

BIOCHEMISTRY;

3ALPHA HYDROXYSTEROID DEHYDROGENASE; ALANINE; ALDEHYDE REDUCTASE; HYDROXYSTEROID DEHYDROGENASE; ISOENZYME; LIGAND; TESTOSTERONE;

ALANINE SCANNING MUTAGENESIS; ARTICLE; BINDING AFFINITY; CATALYSIS; COMPLEX FORMATION; CRYSTAL STRUCTURE; ENERGY TRANSFER; ENZYME ACTIVE SITE; ENZYME KINETICS; ENZYME MECHANISM; ENZYME STRUCTURE; ENZYME SUBSTRATE; FLUORESCENCE; HORMONE BINDING; HORMONE METABOLISM; HORMONE PROTEIN COMPLEX; HYDROPHOBICITY; LIGAND BINDING; MUTAGENESIS; MUTANT; OXIDATION; PRIORITY JOURNAL; TITRIMETRY; WILD TYPE;

3-ALPHA-HYDROXYSTEROID DEHYDROGENASE (B-SPECIFIC); ALANINE; AMINO ACID SUBSTITUTION; ANIMALS; BINDING SITES; CATALYSIS; KINETICS; MODELS, CHEMICAL; MUTAGENESIS, SITE-DIRECTED; NADP; OXIDATION-REDUCTION; PROGESTERONE; PROTEIN BINDING; RATS; RECOMBINANT PROTEINS; SPECTROMETRY, FLUORESCENCE; SPECTROPHOTOMETRY, ULTRAVIOLET; SUBSTRATE SPECIFICITY; TESTOSTERONE;

EID: 2442477858     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi0499563     Document Type: Article

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