Mutagenesis of 3α-hydroxysteroid dehydrogenase reveals a 'push -pull' mechanism for proton transfer in aldo-keto reductases

Biochemistry

Volumn 37, Issue 10, 1998, Pages 3538-3548

  Schlegel, Brian P ^a,b   Jez, Joseph M ^a   Penning, Trevor M ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b BRIGHAM AND WOMEN S HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

3ALPHA HYDROXYSTEROID DEHYDROGENASE; ALDEHYDE REDUCTASE; ANDROSTANOLONE; KETONE; OXIDOREDUCTASE; TESTOSTERONE; TYROSINE;

ARTICLE; ENZYME ACTIVITY; ENZYME MECHANISM; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTON TRANSPORT; RADIOMETRY; SPECTROPHOTOMETRY; STEROID METABOLISM;

3-ALPHA-HYDROXYSTEROID DEHYDROGENASE (B-SPECIFIC); 3-HYDROXYSTEROID DEHYDROGENASES; ALCOHOL OXIDOREDUCTASES; ANIMALS; BASE SEQUENCE; BINDING SITES; DNA PRIMERS; ESCHERICHIA COLI; HUMANS; HYDROGEN-ION CONCENTRATION; KINETICS; MODELS, CHEMICAL; MUTAGENESIS, SITE-DIRECTED; NADP; OXIDATION-REDUCTION; POINT MUTATION; PROTONS; RECOMBINANT PROTEINS; STEROIDS;

ESCHERICHIA COLI; FELIS CATUS;

EID: 0032502245     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi9723055     Document Type: Article

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