Characterization of the substrate binding site in rat liver 3α- hydroxysteroid/dihydrodiol dehydrogenase: The roles of tryptophans in ligand binding and protein fluorescence

Journal of Biological Chemistry

Volumn 271, Issue 47, 1996, Pages 30190-30198

  Jez, Joseph M ^a   Schlegel, Brian P ^a   Penning, Trevor M ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

3ALPHA HYDROXYSTEROID DEHYDROGENASE;

ARTICLE; BINDING AFFINITY; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME KINETICS; FLUORESCENCE SPECTROSCOPY; LIGAND BINDING; NONHUMAN; PRIORITY JOURNAL; RAT; SITE DIRECTED MUTAGENESIS;

3-ALPHA-HYDROXYSTEROID DEHYDROGENASE (B-SPECIFIC); 3-HYDROXYSTEROID DEHYDROGENASES; ALCOHOL OXIDOREDUCTASES; ANIMALS; ENZYME INHIBITORS; FLUORESCENCE; KINETICS; LIGANDS; LIVER; MUTAGENESIS, SITE-DIRECTED; NADP; OXIDOREDUCTASES; OXIDOREDUCTASES ACTING ON CH-CH GROUP DONORS; RATS; SUBSTRATE SPECIFICITY; TESTOSTERONE; TRYPTOPHAN;

EID: 0029860449     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.271.47.30190     Document Type: Article

References (44)

1. Smithgall, T. E., Harvey, R. G.,and Penning, T. M. (1986) J. Biol. Chem.261, 6184-6191
2. Penning, T. M. (1993) Chem. Biol. Interact.89, 1-34
3. Penning, T. M., Ohnishi, T., Ohnishi, T. S.,and Harvery, R. G. (1996) Chem. Res. Toxicol.9, 84-92
4. Pawlowski, J. E., Huizinga, M.,and Penning, T. M. (1991) J. Biol. Chem.266, 8820-8825
5. Deyashiki, Y., Ogasawara, A., Nakayama, T., Nakanishi, M., Miyabe, Y., Sato, K.,and Hara, A. (1994) Biochem. J.299, 545-552
6. Khanna, M., Qin, K.-N., Wang, R. W.,and Cheng, K.-C. (1995) J. Biol. Chem.270, 20162-20168
7. Hoff, H. G.,and Schriefers, H. (1973) Hoppe-Seyler's Z. Physiol. Chem.354, 507-513
8. Bohren, K. M., Bullock, B., Wermuth, B.,and Gabbay, K. H. (1989) J. Biol. Chem.264, 9547-9551
9. Lacy, W. R., Washenick, K. J., Cook, R. G.,and Dunbar, B. S. (1993) Mol. Endocrinol.7, 58-66
10. Bruce, N. C., Willey, D. L., Coulson, A. F. W.,and Jeffery, J. (1994) Biochem. J.299, 805-811
11. Miura, R., Shiota, K., Noda, K., Yagi, S., Ogawa, T.,and Takahashi, M. (1994) Biochem. J.299, 561-567
12. Deyashiki, Y., Ohshima, K., Nakanishi, M., Sato, K., Matsuura, K.,and Hara, A. (1995) J. Biol. Chem.270, 10461-10467
13. Petrash, J. M., Tarle, I., Wilson, D. K.,and Quiocho, F. A. (1995) Diabetes43, 955-959
14. Tomlinson, D. R., Stevens, E. J.,and Diemel, L. T. (1994) Trends Pharmacol. Sci.15, 293-297
15. Penning, T. M.,and Talalay, P. (1982) Proc. Natl. Acad. Sci. U.S.A.80, 4504-4508
16. Hoog, S. S., Pawlowski, J. E., Alzari, P. M., Penning, T. M.,and Lewis, M. (1994) Proc. Natl. Acad. Sci. U. S. A.91, 2517-2521
17. Bennett, M. J., Schlegel, B. P., Jez, J. M., Penning, T. M.,and Lewis, M. (1996) Biochemistry35, 10702-10711
18. Pawlowski, J. E.,and Penning, T. M. (1994) J. Biol. Chem.269, 13502-13510
19. Ricigliano, J. W.,and Penning, T. M. (1990) Biochem. J.269, 749-755
20. Smithgall, T. E.,and Penning, T. M. (1988) Biochem. J.254, 715-721
21. Laemmli, U. K. (1970) Nature227, 680-685
22. Lowry, O. H., Rosebrough, N. J., Farr, A. J.,and Randall, R. J. (1951) J. Biol. Chem.193, 265-275
23. Leatherbarrow, R. J. (1987) ENZFITTER: A Non-linear Regression Data Analysis Program for the IBM PC (and True Compatibles), BioSoft, Cambridge, UK
24. Wilkinson, G. N. (1961) Biochem. J80, 324-332
25. Chou, T. C.,and Talalay, P. (1981) Eur. J. Biochem.115, 207-216
26. Coiro, V. M., Giglio, E., Lucano, A.,and Puliti, R. (1973) Acta. Crystallogr. B29, 1404-1409
27. Permyakov, E. A. (1993) in Luminescent Spectroscopy of Proteins, pp.57-65, CRC Press, Inc., Boca Raton, FL
28. Askonas, L. J., Ricigliano, J. W.,and Penning, T. M. (1991) Biochem. J.278, 835-841
29. Forster, T. (1948) Ann. Phys. (Leipzig)2, 3110-3117
30. Kubiseski, T. J., Hyndman, D. J., Morjana, N. A.,and Flynn, T. G. (1992) J. Biol. Chem.267, 6510-6517
31. Ehrig, T., Bohren, K. M., Prendergast, F. G.,and Gabbay, K. H. (1994) Biochemistry33, 7157-7165
32. Borhani, D. W., Harter, T. M.,and Petrash, J. M. (1992) J. Biol. Chem.267, 24841-24847
33. Penning, T. M., Abrams, W. R.,and Pawlowski, J. E. (1991) J. Biol. Chem.266, 8826-8834
34. Schlegel, B. P., Pawlowski, J. E., Hu, Y., Scolnick, D. M., Covey, D. C.,and Penning, T. M. (1994) Biochemistry33, 10367-10374
35. Wilson D. K., Tarle, I., Petrash, J. M.,and Quiocho, F. A. (1993) Proc. Natl. Acad. Sci. U. S. A.90, 9847-9851
36. Wermuth, B.,and Monder, C. (1983) Eur. J. Biochem.131, 423-426
37. Tete-Favier, F., Barth, P., Mitchler, A., Podjarny, A. D., Rondeau, J. M., Urzhumtsev, A., Biellman, J. F.,and Moras, D. (1995) Eur. J. Med. Chem.30, 589-603
38. Joseph, D., Petsko, G. A.,and Karplus, M. (1990) Science249, 1425-1428
39. Pompliano, D. L., Peyman, A.,and Knowles, J. R. (1990) Biochemistry29, 3186-3194
40. Wierenga, R. K., Noble, M. E. M.,and Davenport, R. C. (1992) J. Mol. Biol.224, 1115-1126
41. Shen, T. Y. (1965) in Non-steroidal Anti-inflammatory Drugs (Garattini, S.,and Dukes, M. N. G., eds) p.13, Excerpta Medical Foundation, New York
42. Kador, P. F.,and Sharpless, N. E. (1983) Mol. Pharmacol.24, 521-531
43. Mylari, B. L., Larson, E. R., Beyer, T. A., Zembrowski, W. J., Aldinger, C. E., Dee, M. F., Siegel, T. W.,and Singleton, D. H. (1991) J. Med. Chem.34, 108-122
44. Evans, S. V. (1990) J. Mol. Graph.12, 134-138.