Alanine scanning of Arp1 delineates a putative binding site for Jnm1/dynamitin and Nip100/p150Glued

Molecular Biology of the Cell

Volumn 16, Issue 9, 2005, Pages 3999-4012

  Clark, Sean W ^a   Rose, Mark D ^a  

^a PRINCETON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; DYNAMITIN; DYNEIN ADENOSINE TRIPHOSPHATASE; PROTEIN; PROTEIN ARP1; PROTEIN NIP100; PROTEIN P150 GLUED; REGULATOR PROTEIN; UNCLASSIFIED DRUG;

ACTIN FILAMENT; ARTICLE; BINDING SITE; GEL FILTRATION; MICROTUBULE; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN FUNCTION;

ACTINS; ALANINE; ALLELES; BASE SEQUENCE; CYTOSKELETAL PROTEINS; MICROTUBULE-ASSOCIATED PROTEINS; MOLECULAR SEQUENCE DATA; MUTATION; NUCLEOTIDES; PHENOTYPE; PROTEIN STRUCTURE, TERTIARY; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 24344440193     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.E05-02-0093     Document Type: Article

References (64)

1. Amberg, D. C., Basart, E., and Botstein, D. (1995). Defining protein interactions with yeast actin in vivo. Nat. Struct. Biol. 2, 28-35.
2. Belli, G., Gari, E., Aldea, M., and Herrero, E. (1998a). Functional analysis of yeast essential genes using a promoter-substitution cassette and the tetracycline-regulatable dual expression system. Yeast 14, 1127-1138.
3. Belli, G., Gari, E., Piedrafita, L., Aldea, M., and Herrero, E. (1998b). An activator/repressor dual system allows tight tetracycline-regulated gene expression in budding yeast. Nucleic Acids Res. 26, 942-947.
4. Belmont, L. D., and Drubin, D. G. (1998). The yeast V159N actin mutant reveals roles for actin dynamics in vivo. J. Cell Biol. 742, 1289-1299.
5. Belmont, L. D., Orlova, A., Drubin, D. G., and Egelman, E. H. (1999a). A change in actin conformation associated with filament instability after Pi release. Proc. Natl. Acad. Sci. USA 96, 29-34.
6. Belmont, L. D., Patterson, G. M., and Drubin, D. G. (1999b). New actin mutants allow further characterization of the nucleotide binding cleft and drug binding sites. J. Cell Sci. 112, 1325-1336.
7. Bingham, J. B., and Schroer, T. A. (1999). Self-regulated polymerization of the actin-related protein Arp1. Curr. Biol. 9, 223-226.
8. Cairns, B. R., Erdjument-Bromage, H., Tempst, P., Winston, F., and Kornberg, R. D. (1998). Two actin-related proteins are shared functional components of the chromatin-remodeling complexes RSC and SWI/SNF. Mol. Cell 2, 639-651.
9. Clark, S. W., and Meyer, D. I. (1992). Centractin is an actin homologue associated with the centrosome. Nature 359, 246-250.
10. Cottingham, F. R., and Hoyt, M. A. (1997). Mitotic spindle positioning in Saccharomyces cerevisiae is accomplished by antagonistically acting microtubule motor proteins. J. Cell Biol. 738, 1041-1053.
11. Cross, F. R. (1997). 'Marker swap' plasmids: convenient tools for budding yeast molecular genetics. Yeast 73, 647-653.
12. DeZwaan, T. M., Ellingson, E., Pellman, D., and Roof, D. M. (1997). Kinesin-related KIP3 of Saccharomyces cerevisiae is required for a distinct step in nuclear migration. J. Cell Biol. 138, 1023-1040.
13. Drubin, D. G., Jones, H. D., and Wertman, K. F. (1993). Actin structure and function: roles in mitochondrial organization and morphogenesis in budding yeast and identification of the phalloidin-binding site. Mol. Biol. Cell 4, 1277-1294.
14. Eckley, D. M., Gill, S. R., Melkonian, K. A., Bingham, J. B., Goodson, H. V., Heuser, J. E., and Schroer, T. A. (1999). Analysis of dynactin subcomplexes reveals a novel actin-related protein associated with the arp1 minifilament pointed end. J. Cell Biol. 147, 307-320.
15. Eckley, D. M., and Schroer, T. A. (2003). Interactions between the evolutionarily conserved, actin-related protein, Arp11, actin, and Arp1. Mol. Biol. Cell 14, 2645-2654. Epub 2003 Mar 2620.
16. Garces, J. A., Clark, I. B., Meyer, D. I., and Vallee, R. B. (1999). Interaction of the p62 subunit of dynactin with Arp1 and the cortical actin cytoskeleton. Curr. Biol. 9, 1497-1500.
17. Gietz, R. D., and Schiestl, R. H. (1995). Transforming yeast cells with DNA. Mol. Cell. Biol. 5, 255-269.
18. Goodson, H. V., and Hawse, W. F. (2002). Molecular evolution of the actin family. J. Cell Sci. 115, 2619-2622.
19. Guex, N., and Peitsch, M. C. (1997). SWISS-MODEL and the Swiss-Pdb-Viewer: an environment for comparative protein modeling. Electrophoresis 18, 2714-2723.
20. Hodgkinson, J. L., Peters, C., Kuznetsov, S. A., and Steffen, W. (2005). Three-dimensional reconstruction of the dynactin complex by single-particle image analysis. Proc. Natl. Acad. Sci. USA 102, 3667-3672. Epub 2005 Feb 3628.
21. Holleran, E. A., Karki, S., and Holzbaur, E. L. (1998). The role of the dynactin complex in intracellular motility. Int. Rev. Cytol. 182, 69-109.
22. Holton, T. A., and Graham, M. W. (1991). A simple and efficient method for direct cloning of PCR products using ddT-tailed vectors. Nucleic Acids Res. 19, 1156
23. Holtzman, D. A., Wertman, K. F., and Drubin, D. G. (1994). Mapping actin surfaces required for functional interactions in vivo. J. Cell Biol. 126, 423-432.
24. James, P., Halladay, J., and Craig, E. A. (1996). Genomic libraries and a host strain designed for highly efficient two-hybrid selection in yeast. Genetics 144, 1425-1436.
25. Jensen, R. B., and Gerdes, K. (1997). Partitioning of plasmid R1. The ParM protein exhibits ATPase activity and interacts with the centromere-like ParR-parC complex. J. Mol. Biol. 269, 505-513.
26. Kahana, J. A., Schlenstedt, G., Evanchuk, D. M., Geiser, J. R., Hoyt, M. A., and Silver, P. A. (1998). The yeast dynactin complex is involved in partitioning the mitotic spindle between mother and daughter cells during anaphase B. Mol. Biol. Cell 9, 1741-1756.
27. Karki, S., and Holzbaur, E. L. (1999). Cytoplasmic dynein and dynactin in cell division and intracellular transport. Curr. Opin. Cell Biol. 33, 45-53.
28. Kern, L., de Montigny, J., Jund, R., and Lacroute, F. (1990). The FUR1 gene of Saccharomyces cerevisiae: cloning, structure and expression of wild-type and mutant alleles. Gene 88, 149-157.
29. Korinek, W. S., Copeland, M. J., Chaudhuri, A., and Chant, J. (2000). Molecular linkage underlying microtubule orientation toward cortical sites in yeast. Science 287, 2257-2259.
30. Kumar, S., Zhou, Y., and Plamann, M. (2001). Dynactin-membrane interaction is regulated by the C-terminal domains of p150(Glued). EMBO Rep. 2, 939-944. Epub 2001 Sep 2024.
31. Kunkel, T. A. (1985). Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. USA 82, 488-492.
32. Lee, I. H., Kumar, S., and Plamann, M. (2001). Null mutants of the Neurospora actin-related protein 1 pointed-end complex show distinct phenotypes. Mol. Biol. Cell 32, 2195-2206.
33. Lee, L., Tirnauer, J. S., Li, J., Schuyler, S. C., Liu, J. Y., and Pellman, D. (2000). Positioning of the mitotic spindle by a cortical-microtubule capture mechanism. Science 287, 2260-2262.
34. Lees-Miller, J. P., Helfman, D. M., and Schroer, T. A. (1992). A vertebrate actin-related protein is a component of a multisubunit complex involved in microrubule-based vesicle motility. Nature 359, 244-246.
35. Lorenz, M., Popp, D., and Holmes, K. C. (1993). Refinement of the F-actin model against X-ray fiber diffraction data by the use of a directed mutation algorithm. J. Mol. Biol. 234, 826-836.
36. Miller, C. J., Cheung, P., White, P., and Reisler, E. (1995). Actin's view of actomyosin interface. Biophys. J. 68, 50S-54S.
37. Miller, R. K., Cheng, S. C., and Rose, M. D. (2000). Bim1p/Yeb1p mediates the Kar9p-dependent cortical attachment of cytoplasmic microtubules. Mol. Biol. Cell 33, 2949-2959.
38. Miller, R. K., Heller, K. K., Frisen, L., Wallack, D. L., Loayza, D., Gammie, A. E., and Rose, M. D. (1998). The kinesin-related proteins, Kip2p and Kip3p, function differently in nuclear migration in yeast. Mol. Biol. Cell 9, 2051-2068.
39. Milligan, R. A. (1996). Protein-protein interactions in the rigor actomyosin complex. Proc. Natl. Acad. Sci. USA 93, 21-26.
40. Minke, P. F., Lee, I. H., Tinsley, J. H., Bruno, K. S., and Plamann, M. (1999). Neurospora crassa ro-10 and ro-11 genes encode novel proteins required for nuclear distribution. Mol. Microbiol. 32, 1065-1076.
41. Minke, P. F., Lee, I. H., Tinsley, J. H., and Plamann, M. (2000). A Neurospora crassa Arp1 mutation affecting cytoplasmic dynein and dynactin localization. Mol. Gen. Genet. 264, 433-440.
42. Muresan, V., Stankewich, M. C., Steffen, W., Morrow, J. S., Holzbaur, E. L., and Schnapp, B. J. (2001). Dynactin-dependent, dynein-driven vesicle transport in the absence of membrane proteins: a role for spectrin and acidic phospholipids. Mol. Cell 7, 173-183.
43. Peitsch, M. C. (1996). ProMod and Swiss-Model: Internet-based tools for automated comparative protein modelling. Biochem. Soc. Trans. 24, 274-279.
44. Poch, O., and Winsor, B. (1997). Who's who among the Saccharomyces cerevisiae actin-related proteins? A classification and nomenclature proposal for a large family. Yeast 33, 1053-1058.
45. Pollard, T. D., and Cooper, J. A. (1986). Actin and actin-binding proteins. A critical evaluation of mechanisms and functions. Annu. Rev. Biochem. 55, 987-1035.
46. Puls, I., et al. (2003). Mutant dynactin in motor neuron disease. Nat. Genet. 33, 455-456.
47. Rayment, I., Holden, H. M., Whittaker, M., Yohn, C. B., Lorenz, M., Holmes, K. C., and Milligan, R. A. (1993). Structure of the actin-myosin complex and its implications for muscle contraction. Science 261, 58-65.
48. Reijo, R. A., Cooper, E. M., Beagle, G. J., and Huffaker, T. C. (1994). Systematic mutational analysis of the yeast beta-tubulin gene. Mol. Biol. Cell 5, 29-43.
49. Robinson, R. C., Turbedsky, K., Kaiser, D. A., Marchand, J. B., Higgs, H. N., Choe, S., and Pollard, T. D. (2001). Crystal structure of arp2/3 complex. Science 294, 1679-1684.
50. Rose, M. D., Novick, P., Thomas, J. H., Botstein, D., and Fink, G. R. (1987). A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector. Gene 60, 237-243.
51. Rose, M. D., Winston, F., and Hieter, P. (1990). Methods in Yeast Genetics, Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
52. Salina, D., Bodoor, K., Eckley, D. M., Schroer, T. A., Rattner, J. B., and Burke, B. (2002). Cytoplasmic dynein as a facilitator of nuclear envelope breakdown. Cell 308, 97-107.
53. Sasaki, N., and Sutoh, K. (1998). Structure-mutation analysis of the ATPase site of Dictyostelium discoideum myosin II. Adv. Biophys. 35, 1-24.
54. Schafer, D. A., Gill, S. R., Cooper, J. A., Heuser, J. E., and Schroer, T. A. (1994). Ultrastructural analysis of the dynactin complex: an actin-related protein is a component of a filament that resembles F-actin. J. Cell Biol. 126, 403-412.
55. Schafer, D. A., and Schroer, T. A. (1999). Actin-related proteins. Annu. Rev. Cell Dev. Biol. 15, 341-363.
56. Schroder, R. R., Manstein, D. J., Jahn, W., Holden, H., Rayment, I., Holmes, K. C., and Spudich, J. A. (1993). Three-dimensional atomic model of F-actin decorated with Dictyostelium myosin S1. Nature 364, 171-174.
57. Schroer, T. A. (2004). Dynactin. Annu. Rev. Cell Dev. Biol. 20, 759-779.
58. Sikorski, R. S., and Hieter, P. (1989). A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 322, 19-27.
59. Studier, F. W., and Moffatt, B. A. (1986). Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 389, 113-130.
60. Wach, A., Brachat, A., Pohlmann, R., and Philippsen, P. (1994). New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast 10, 1793-1808.
61. Wertman, K. F., Drubin, D. G., and Botstein, D. (1992). Systematic mutational analysis of the yeast ACT1 gene. Genetics 332, 337-350.
62. Whitacre, J., Davis, D., Toenjes, K., Brower, S., and Adams, A. (2001). Generation of an isogenic collection of yeast actin mutants and identification of three interrelated phenotypes. Genetics 357, 533-543.
63. Woehlke, G., Ruby, A. K., Hart, C. L., Ly, B., Hom-Booher, N., and Vale, R. D. (1997). Microtubule interaction site of the kinesin motor. Cell 90, 207-216.
64. Yin, H., Pruyne, D., Huffaker, T. C., and Bretscher, A. (2000). Myosin V orientates the mitotic spindle in yeast. Nature 406, 1013-1015.