Neurospora crassa ro-10 and ro-11 genes encode novel proteins required for nuclear distribution

Molecular Microbiology

Volumn 32, Issue 5, 1999, Pages 1065-1076

  Minke, Peter F ^a   Lee, In Hyung ^b   Tinsley, John H ^c   Bruno, Kenneth S ^d   Plamann, Michael ^b  

^a TEXAS A AND M UNIVERSITY   (United States)

^b UNIVERSITY OF MISSOURI KANSAS CITY   (United States)

^c TEXAS A AND M HEALTH SCIENCE CENTER   (United States)

^d PURDUE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DYNEIN ADENOSINE TRIPHOSPHATASE; FUNGAL PROTEIN; PROTEIN SUBUNIT;

ARTICLE; CELL NUCLEUS; CYTOSKELETON; FUNGAL GENETICS; GENE ISOLATION; GENE MUTATION; MICROTUBULE; NEUROSPORA CRASSA; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN LOCALIZATION;

AMINO ACID SEQUENCE; BLOTTING, WESTERN; CELL NUCLEUS; CLONING, MOLECULAR; DYNEIN ATPASE; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; FUNGAL PROTEINS; GENE DELETION; GENES, FUNGAL; MICROTUBULE-ASSOCIATED PROTEINS; MICROTUBULES; MOLECULAR SEQUENCE DATA; NEUROSPORA CRASSA; RESTRICTION MAPPING; SEQUENCE ANALYSIS, DNA;

FUNGI; NEUROSPORA CRASSA;

EID: 0032984997     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1999.01421.x     Document Type: Article

References (44)

1. Altschul, S.F., Gish, W., Miller, W., Myers, E.W., and Lipman, D.J. (1990) Basic local alignment search tool. J Mol Biol 215: 403-410.
2. Bruno, K.S., Tinsley, J.H., Minke, P.F., and Plamann, M. (1996) Genetic interactions among cytoplasmic dynein, dynactin, and nuclear distribution mutants of Neurospora crassa. Proc Natl Acad Sci USA 93: 4775-4780.
3. Cid, V.J., Durán, A., del Rey, F., Snyder, M.P., Nombela, C., and Sanchez, M. (1995) Molecular basis of cell integrity and morphogenesis in Saccharomyces cerevisiae. Microbiol Rev 59: 345-386.
4. Clark, S.W., and Meyer, D.I. (1994) Act3: a putative centractin homologue in S. cerevisiae is required for proper spindle orientation of the mitotic spindle. J Cell Biol 127: 129-138.
5. Cullen, D., Leong, S.A., Wilson, L.J., and Henner, D.J. (1987) Transformation of Aspergillus nidulans with the hygromycin-resistance gene, hph. Gene 57: 21-26.
6. Davis, R.H., and de Serres, F.J. (1970) Genetic and microbiological research techniques for Neurospora crassa. Methods Enzymol 27A: 79-143.
7. Devereux, J., Haeberli, P., and Smithies, O. (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12: 387-395.
8. Eshel, D., Urrestarazu, L.A., Vissers, S., Jauniaux, J.-C., van Vliet-Reedijk, J.C., Planta, R.J., et al. (1993) Cytoplasmic dynein is required for normal nuclear segregation in yeast. Proc Natl Acad Sci USA 90: 11172-11176.
9. Garnjobst, L., and Tatum, E.L. (1967) A survey of new morphological mutants in Neurospora crassa. Genetics 57: 579-604.
10. Gill, S.R., Schroer, T.A., Szilak, I., Steuer, E.R., Sheetz, M.P., and Cleveland, D.W. (1991) Dynactin, a conserved, ubiquitously expressed component of an activator of vesicle motility mediated by cytoplasmic dynein. J Cell Biol 115: 1639-1650.
11. Holleran, E.A., Tokio, M.K., Karki, S., and Holzbaur, E.L.F. (1996) Centractin (ARP1) associates with spectrin revealing a potential mechanism to link dynactin to intracellular organelles. J Cell Biol 135: 1815-1829.
12. Holzbaur, E.L.F., and Vallee, R.B. (1994) Dyneins: molecular structure and cellular function. Annu Rev Cell Biol 10: 339-372.
13. Huffaker, T.C., Thomas, J.H., and Botstein, D. (1988) Diverse effects of β-tubulin mutations on microtubule formation and function. J Cell Biol 106: 1997-2010.
14. Karki, S., Lamonte, B., and Holzbaur, E.L.F. (1998) Characterization of the p22 subunit of dynactin reveals the localization of cytoplasmic dynein and dynactin to the midbody of dividing cells. J Cell Biol 142: 1023-1034.
15. Knight, A.E., and Kendrick-Jones, J. (1993) A myosin-like protein from a higher plant. J Mol Biol 231: 148-154.
16. Li, Y.-Y., Yeh, E., Hays, T., and Bloom, K. (1993) Disruption of mitotic spindle orientation in a yeast dynein mutant. Proc Natl Acad Sci 90: 10096-10100.
17. Löpez-Franco, R., Bratnicki-Garcia, S., and Bracker, C.E. (1994) Pulsed growth of fungal hyphal tips. Proc Natl Acad Sci 91: 12228-12232.
18. Lupas, A., Dyke, M.V., and Stock, J. (1991) Predicting coiled coils from protein sequence. Science 252: 1162-1164.
19. McKerracher, L.J., and Heath, I.B. (1987) Cytoplasmic migration and intracellular organelle movements during tip growth of fungal hyphae. Exp Mycol 11: 79-100.
20. Metzenberg, R.L., Stevens, J.N., Selker, E.U., and Morzycka-Wroblewska, E. (1985) Identification and chromosomal distribution of 5S rRNA genes in Neurospora crassa. Proc Natl Acad Sci USA 82: 2067-2071.
21. Morris, N.R., Xiang, X., and Beckwith, S.M. (1995) Nuclear migration advances in fungi. Trends Cell Biol 5: 278-282.
22. Muhua, L., Karpova, T.S., and Cooper, J.A. (1994) A yeast actin-related protein homologous to that in vertebrate dynactin complex is important for spindle orientation and nuclear migration. Cell 78: 669-679.
23. Oakley, B.R., and Morris, N.R. (1980) Nuclear movement is β-tubulin-dependent in Aspergillus nidulans. Cell 19: 255-262.
24. Oakley, B.R., Oakley, C.E., Yoon, Y., and Jung, M.K. (1990) γ-tubulin is a component of the spindle pole body that is essential for microtubule function in Aspergillus nidulans. Cell 61: 1289-1301.
25. Palmer, R.E., Sullivan, D.S., Huffaker, T., and Koshland, D. (1992) Role of astral microtubules and actin in spindle orientation and migration in the budding yeast, Saccharomyces cerevisiae. J Cell Biol 119: 583-593.
26. Perkins, D.D., Radford, A., Newmyer, D., and Björkman, M. (1982) Chromosomal loci of Neurospora crassa. Microbiol Rev 46: 426-570.
27. Pfister, K.K., Benashski, S.E., Dillman, J.F., Patelking, R.S., and King, S.M. (1998) Identification and molecular characterization of the p24 dynactin light chain. Cell Motil Cytoskel 41: 154-167.
28. Plamann, M., Minke, P.F., Tinsley, J.H., and Bruno, K.S. (1994) Cytoplasmic dynein and actin-related protein Arp1 are required for normal nuclear distribution in filamentous fungi. J Cell Biol 127: 139-149.
29. Raudaskoski, M., Rupes, I., andTimonen, S. (1991) Immunofluorescence microscopy of the cytoskeleton in filamentous fungi after quick-freezing and low-temperature fixation. Exp Mycol 15: 167-173.
30. Robb, M.J., Wilson, M.A., and Vierula, P.J. (1995) A fungal actin-related protein involved in nuclear migration. Mol Gen Genet 247: 583-590.
31. Sambrook, J., Fritsch, E.H., and Maniatis, T. (1989) Molecular Cloning. A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
32. Sanger, F., Nicklen, S., and Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463-5467.
33. Schafer, D.A., Gill, S.R., Cooper, J.A., Heuser, J.E., and Schroer, T.A. (1994) Ultrastructural analysis of the dynactin complex: an actin-related protein is a component of a filament that resembles F-actin. J Cell Biol 126: 403-412.
34. Schroer, T.A., and Sheetz, M.P. (1991) Two activators of microtubule-based vesicle transport. J Cell Biol 115: 1309-1318.
35. Stukenberg, P.T., Lustig, K.D., McGarry, T.J., King, R.W., Kuang, J., and Kirschner, M.W. (1997) Systematic identification of mitotic phosphoproteins. Curr Biol 7: 338-348.
36. That, T.C., Rossier, C., Barja, F., Turian, G., and Roos, U. (1988) Induction of multiple germ tubes in Neurospora crassa by antitubulin agents. Eur J Cell Biol 46: 68-79.
37. Thompson-Coffe, C., and Zickler, D. (1994) How the cytoskeleton recognizes and sorts nuclei of opposite mating type during the sexual cycle in filamentous ascomycetes. Dev Biol 165: 257-271.
38. Glued, the largest subunit of the dynactin complex, is nonessential in Neurospora but required for nuclear distribution. Mol Biol Cell 7: 731-742.
39. Towbin, H., Staehelin, T., and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350-4354.
40. Vollmer, S.J., and Yanofsky, C. (1986) Efficient cloning of genes of Neurospora crassa. Proc Natl Acad Sci USA 83: 4869-4873.
41. Willins, D.A., Liu, B., Xiang, X., and Morris, N.R. (1997) Mutations in the heavy chain of cytoplasmic dynein suppress the nudF nuclear migration mutation of Aspergillus nidulans. Mol Gen Genet 255: 194-200.
42. Xiang, X., Beckwith, S.M., and Morris, N.R. (1994) Cytoplasmic dynein is involved in nuclear migration in Aspergillus nidulans. Proc Natl Acad Sci USA 91: 2100-2104.
43. Xiang, X., Roghi, C., and Morris, N.R. (1995) Characterization and localization of the cytoplasmic dynein heavy chain in Aspergillus nidulans. Proc Natl Acad Sci USA 92: 9890-9894.
44. Yarden, O., Plamann, M., Ebbole, D.J., and Yanofsky, C. (1992) cot-1, a gene required for hyphal elongation in Neurospora crassa, encodes a protein kinase. EMBO J 11: 2159-2166.