Efficient system of artificial oil bodies for functional expression and purification of recombinant nattokinase in Escherichia coli

Journal of Agricultural and Food Chemistry

Volumn 53, Issue 12, 2005, Pages 4799-4804

  Chiang, Chung Jen ^a   Chen, Hong Chen ^a   Chad, Yun Peng ^b   Tzen, Jason T C ^a  

^a NATIONAL CHUNG HSING UNIVERSITY   (Taiwan)

^b FENG CHIA UNIVERSITY   (Taiwan)

Author keywords

Artificial oil body; Intein; Nattokinase; Oleosin; Propeptide

Indexed keywords

ENZYME PRECURSOR; HYBRID PROTEIN; INTEIN; NATTOKINASE; SUBTILISIN; VEGETABLE OIL; VEGETABLE PROTEIN;

ARTICLE; BACILLUS SUBTILIS; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; PLANT SEED; SOLUBILITY;

BACILLUS SUBTILIS; ENZYME PRECURSORS; ESCHERICHIA COLI; GENE EXPRESSION; INTEINS; PLANT OILS; PLANT PROTEINS; RECOMBINANT FUSION PROTEINS; SEEDS; SOLUBILITY; SUBTILISINS;

ESCHERICHIA COLI;

EID: 21644451024     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf050264a     Document Type: Article

References (27)

1. Hockney, R. C. Recent development in heterologous protein production in Escherichia coli. Trends Biotechnol. 1994, 12, 456-463.
2. Hearn, M. T. W.; Acosta, D. Applications of novel affinity cassette methods: use of peptide fusion handles for the purification of recombinant proteins. J. Mol. Recognit. 2001, 14, 323-369.
3. Terpe, K. Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Appl. Microbiol. Biotechnol. 2003, 60, 523-533.
4. Schauer-Vukasionovic, V.; Deo, S. K.; Daunet, S. Purification method for recombinant proteins based on a fusion between the target protein and the C-terminus of calmodulin. Anal. Bioanal. Chem. 2002, 373, 501-507.
5. Chen, J. C. F.; Tsai, C. C. Y.; Tzen, J. T. C. Cloning and secondary structure analysis of caleosin, a unique calcium-binding protein in oil bodies of plant seeds. Plant Cell Physiol. 1999, 40, 1079-1086.
6. Lin, L. J.; Tai, S. S. K.; Peng, C. C.; Tzen, J. T. C. Steroleosin, a sterol-binding dehydrogenase in seed oil bodies. Plant Physiol. 2002, 128, 1200-1211.
7. Tai, S. S. K.; Chen, M. C. M.; Peng, C. C.; Tzen, J. T. C. Gene family of oleosin isoforms in sesame seed oil bodies and their structural stabilization to reconstituted oil bodies. Biosci., Biotechnol., Biochem. 2002, 66, 2146-2153.
8. Frandsen, G. I.; Mundy, J.; Tzen, J. T. C. Oil bodies and their associated proteins, oleosin and caleosin. Physiol. Plant. 2001, 112, 301-307.
9. Van Rooijen, G. J. H.; Moloney, M. M. Plant seed oil-bodies as carriers for foreign proteins. Bio/Technology 1995, 13, 72-77.
10. Peng, C. C.; Chen, J. C. F.; Shyu, D. J. H.; Chen, M. J.; Tzen, J. T. C. A system for purification of recombinant proteins in Escherichia coli via artificial oil bodies constituted with their oleosin-fused polypeptides. J. Biotechnol 2004, 111, 51-57.
11. +-adenosine triphosphatase. Science 1990, 250, 651-657.
12. Chong, S.; Yang, S.; Paulus, H.; Benner, J.; Perler, F. B.; Xu, M. Q. Protein splicing involving the Saccharomyces cerevisiae VMA intein: the steps in the splicing pathway, side reactions leading to protein cleavage, and establishment of an in vitro splicing system. J. Biol. Chem. 1996, 271, 22159-22168.
13. Mathys, S.; Evans, T. C., Jr.; Chute, I. C.; Wu, H.; Chong, S.; Benner, J.; Liu, X. Q.; Xu, M. Q. Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation. Gene 1999, 231, 1-13.
14. Southworth, M. W.; Amaya, K.; Evans, T. C.; Xu, M. Q.; Perler, F. B. Purification of proteins fused to either the amino or carboxy terminus of the Mycobacterium xenopi gyrase A intein. Bio-Techniques 1999, 27, 110-120.
15. Telenti, A.; Southworth, M.; Alcaide, F.; Daugelat, S.; Jacobs, W. R.; Perler, F. B. The Mycobacterium xenopi GyrA proteins splicing element: characterization of a minimal intein. J. Bacteriol. 1997, 179, 6378-6382.
16. Urano, T.; Hhara, H.; Umemura, K.; Suzuki, Y.; Oike, M.; Akita, S.; Tsukamoto, Y.; Suzuki, I.; Takada, A. The profibrinolytic enzyme subtilisin NAT purified from Bacillus subtilis cleaves and inactivates plaminogen activator inhibitor type 1. J. Biol Chem. 2001, 276, 24690-24696.
17. Wang, Z. W.; Law, W. S.; Chao, Y. P. Improvement of the thermoregulated T7 expression system by using the heat-sensitive lacI. Biotechnol. Prog. 2004, 20, 1352-1358.
18. Love, C. A.; Lilley, P. E.; Dixon, N. E. Stable high-copy-number bacteriophage A promoter vectors for overproduction of proteins in Escherichia coli. Gene 1996, 176, 49-53.
19. Peng, C. C.; Shyu, D. J. H.; Chou, W. M.; Chen, M. J.; Tzen, J. T. C. Method for bacterial expression and purification of sesame cystatin via artificial oil bodies. J. Agric. Food Chem. 2004, 52, 3115-3119.
20. Miller, J. H. Experiments in Molecular Genetics; Cold Spring Harbor Laboratory: Cold Spring Harbor, NY, 1972.
21. Peng, C. C.; Lin, I. P.; Lin, C. K.; Tzen, J. C. T. Size and stability of reconstituted sesame oil bodies. Biotechnol. Prog. 2003, 19, 1623-1626.
22. Ikemura, H.; Takagi, H.; Inouye, M. Requirement of pro-sequence for the production of active subtilisin E in Escherichia coli. J. Biol. Chem. 1987, 262, 7859-7864.
23. Ikemura, H.; Inouye, M. In vitro processing of pro-subtilisin produced in Escherichia coli. J. Biol. Chem. 1988, 263, 12959-12963.
24. Shinde, U.; Fu, X.; Inouye, M. A pathway for conformational diversity in proteins mediated by intramolecular chaperones. J. Biol. Chem. 1999, 274, 15615-15621.
25. Fu, X.; Inouye, M.; Shinde, U. Folding pathway mediated by an intramolecular chaperone. J. Biol. Chem. 2000, 276, 16871 -16878.
26. Li, Y.; Inouye, M. The mechanism of autoprocessing of the propeptide of prosubtilisin E: intramolecular or intermolecular event. J. Mol. Biol. 1996, 262, 591-594.
27. Li, M.; Su, Z. G.; Janson, J. C. In vitro protein refolding by Chromatographic procedures. Protein Expression Purif. 2004, 33, 1-10.