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Volumn 373, Issue 6, 2002, Pages 501-507
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Purification method for recombinant proteins based on a fusion between the target protein and the C-terminus of calmodulin
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Author keywords
Affinity purification; C terminal fusion; Calmodulin; Factor Xa cleavage; GFP
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Indexed keywords
ESCHERICHIA COLI;
FLUORESCENCE;
PROTEINS;
SILICA;
CLEAVAGE SITES;
PURIFICATION;
BLOOD CLOTTING FACTOR 10A;
BUFFER;
CALCIUM;
CALCIUM CHELATING AGENT;
CALMODULIN;
EGTAZIC ACID;
GREEN FLUORESCENT PROTEIN;
HYBRID PROTEIN;
PEPTIDE FRAGMENT;
PHENOTHIAZINE DERIVATIVE;
PHOTOPROTEIN;
PRIMER DNA;
PROTEIN KINASE (CALCIUM,CALMODULIN);
RECOMBINANT PROTEIN;
SILICON DIOXIDE;
AFFINITY CHROMATOGRAPHY;
AMINO ACID SEQUENCE;
ARTICLE;
CARBOXY TERMINAL SEQUENCE;
CHEMICAL BINDING;
CHEMISTRY;
CONFORMATIONAL TRANSITION;
ESCHERICHIA COLI;
EXTRACT;
FLUORESCENCE;
GENETICS;
METHODOLOGY;
MOLECULAR CLONING;
NUCLEOTIDE SEQUENCE;
PLASMID;
POLYACRYLAMIDE GEL ELECTROPHORESIS;
PROTEIN BINDING;
PROTEIN DEGRADATION;
PROTEIN EXPRESSION;
PROTEIN PROTEIN INTERACTION;
PURIFICATION;
SENSITIVITY AND SPECIFICITY;
SPECTROFLUOROMETRY;
TECHNIQUE;
ISOLATION AND PURIFICATION;
NASA DISCIPLINE LIFE SCIENCES TECHNOLOGIES;
NON-NASA CENTER;
NASA DISCIPLINE LIFE SCIENCES TECHNOLOGIES;
NON-NASA CENTER;
BASE SEQUENCE;
CALMODULIN;
CLONING, MOLECULAR;
DNA PRIMERS;
ELECTROPHORESIS, POLYACRYLAMIDE GEL;
ESCHERICHIA COLI;
FACTOR XA;
GREEN FLUORESCENT PROTEINS;
LUMINESCENT PROTEINS;
PEPTIDE FRAGMENTS;
PLASMIDS;
RECOMBINANT FUSION PROTEINS;
RECOMBINANT PROTEINS;
SENSITIVITY AND SPECIFICITY;
SPECTROMETRY, FLUORESCENCE;
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EID: 0035984545
PISSN: 16182642
EISSN: None
Source Type: Journal
DOI: 10.1007/s00216-002-1351-6 Document Type: Article |
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Times cited : (12)
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References (30)
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