Prohepcidin localises to the Golgi compartment and secretory pathway in hepatocytes

Journal of Hepatology

Volumn 43, Issue 4, 2005, Pages 720-728

  Wallace, Daniel F ^a   Summerville, Lesa ^a   Lusby, Patricia E ^a   Subramaniam, V Nathan ^a  

^a QIMR BERGHOFER MEDICAL RESEARCH INSTITUTE   (Australia)

Author keywords

Hepatocytes; Iron homeostasis; Iron overload; Regulatory hormone; Subcellular localization

Indexed keywords

BETA 2 MICROGLOBULIN; HEPCIDIN; HFE PROTEIN; PROHEPCIDIN; PROTEIN ANTIBODY; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL LINE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; DISORDERS OF METAL METABOLISM; GOLGI COMPLEX; IRON STORAGE; KNOCKOUT MOUSE; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; WILD TYPE;

ANIMALS; ANTIMICROBIAL CATIONIC PEPTIDES; BASE SEQUENCE; BETA 2-MICROGLOBULIN; CLONING, MOLECULAR; DNA PRIMERS; GOLGI APPARATUS; HEPATOCYTES; IMMUNOBLOTTING; MICE; MICE, INBRED C57BL; MICE, KNOCKOUT; RECOMBINANT PROTEINS;

EID: 21044450599     PISSN: 01688278     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jhep.2005.02.047     Document Type: Article

References (34)

1. C.H. Park, E.V. Valore, A.J. Waring, and T. Ganz Hepcidin, a urinary antimicrobial peptide synthesized in the liver J Biol Chem 276 2001 7806 7810
2. A. Krause, S. Neitz, H.J. Magert, A. Schulz, W.G. Forssmann, and P. Schulz-Knappe LEAP-1, a novel highly disulfide-bonded human peptide, exhibits antimicrobial activity FEBS Lett 480 2000 147 150
3. C. Pigeon, G. Ilyin, B. Courselaud, P. Leroyer, B. Turlin, and P. Brissot A new mouse liver-specific gene, encoding a protein homologous to human antimicrobial peptide hepcidin, is overexpressed during iron overload J Biol Chem 276 2001 7811 7819
4. G. Nicolas, M. Bennoun, I. Devaux, C. Beaumont, B. Grandchamp, and A. Kahn Lack of hepcidin gene expression and severe tissue iron overload in upstream stimulatory factor 2 (USF2) knockout mice Proc Natl Acad Sci USA 98 2001 8780 8785
5. G. Nicolas, M. Bennoun, A. Porteu, S. Mativet, C. Beaumont, and B. Grandchamp Severe iron deficiency anemia in transgenic mice expressing liver hepcidin Proc Natl Acad Sci USA 99 2002 4596 4601
6. A. Roetto, G. Papanikolaou, M. Politou, F. Alberti, D. Girelli, and J. Christakis Mutant antimicrobial peptide hepcidin is associated with severe juvenile hemochromatosis Nat Genet 33 2003 21 22
7. G. Papanikolaou, M.E. Samuels, E.H. Ludwig, M.L. MacDonald, P.L. Franchini, and M.P. Dube Mutations in HFE2 cause iron overload in chromosome 1q-linked juvenile hemochromatosis Nat Genet 36 2004 77 82
8. K.R. Bridle, D.M. Frazer, S.J. Wilkins, J.L. Dixon, D.M. Purdie, and D.H. Crawford Disrupted hepcidin regulation in HFE-associated haemochromatosis and the liver as a regulator of body iron homoeostasis Lancet 361 2003 669 673
9. K.A. Ahmad, J.R. Ahmann, M.C. Migas, A. Waheed, R.S. Britton, and B.R. Bacon Decreased liver hepcidin expression in the Hfe knockout mouse Blood Cells Mol Dis 29 2002 361 366
10. H. Kawabata, R.E. Fleming, D. Gui, S.Y. Moon, T. Saitoh, and J. O'Kelly Expression of hepcidin is down-regulated in TfR2 mutant mice manifesting a phenotype of hereditary hemochromatosis Blood 2004 Sep 2 [Epub ahead of print]
11. E. Nemeth, A. Roetto, G. Garozzo, T. Ganz, and C. Camaschella Hepcidin is decreased in TFR2-Hemochromatosis Blood 2004 Oct 14 [Epub ahead of print]
12. E. Nemeth, S. Rivera, V. Gabayan, C. Keller, S. Taudorf, and B.K. Pedersen IL-6 mediates hypoferremia of inflammation by inducing the synthesis of the iron regulatory hormone hepcidin J Clin Invest 113 2004 1271 1276
13. C.N. Roy, A.O. Custodio, J. de Graaf, S. Schneider, I. Akpan, and L.K. Montross An Hfe-dependent pathway mediates hyposideremia in response to lipopolysaccharide-induced inflammation in mice Nat Genet 36 2004 481 485
14. D.M. Frazer, S.J. Wilkins, K.N. Millard, A.T. McKie, C.D. Vulpe, and G.J. Anderson Increased hepcidin expression and hypoferraemia associated with an acute phase response are not affected by inactivation of HFE Br J Haematol 126 2004 434 436
15. P. Lee, H. Peng, T. Gelbart, and E. Beutler The IL-6- and lipopolysaccharide-induced transcription of hepcidin in HFE-, transferrin receptor 2-, and beta 2-microglobulin-deficient hepatocytes Proc Natl Acad Sci USA 101 2004 9263 9265
16. A.H. Laftah, B. Ramesh, R.J. Simpson, N. Solanky, S. Bahram, and K. Schumann Effect of hepcidin on intestinal iron absorption in mice Blood 103 2004 3940 3944
17. S. Yamaji, P. Sharp, B. Ramesh, and S.K. Srai Inhibition of iron transport across human intestinal epithelial cells by hepcidin Blood 104 2004 2178 2180
18. S. Rivera, L. Liu, E. Nemeth, V. Gabayan, O.E. Sorensen, and T. Ganz Hepcidin excess induces the sequestration of iron and exacerbates tumor-associated anemia Blood 2004 Oct 12 [Epub ahead of print]
19. E. Nemeth, M.S. Tuttle, J. Powelson, M.B. Vaughn, A. Donovan, and D.M. Ward Hepcidin regulates iron efflux by binding to ferroportin and inducing its internalization Science 2004 Oct 28 [Epub ahead of print]
20. H. Kulaksiz, S.G. Gehrke, A. Janetzko, D. Rost, T. Bruckner, and B. Kallinowski Pro-hepcidin: expression and cell specific localisation in the liver and its regulation in hereditary haemochromatosis, chronic renal insufficiency, and renal anaemia Gut 53 2004 735 743
21. L. Liu, and T. Ganz The pro region of human neutrophil defensin contains a motif that is essential for normal subcellular sorting Blood 85 1995 1095 1103
22. T. Ganz Biosynthesis of defensins and other antimicrobial peptides Ciba Found Symp 186 1994 62 71
23. E.V. Valore, E. Martin, S.S. Harwig, and T. Ganz Intramolecular inhibition of human defensin HNP-1 by its propiece J Clin Invest 97 1996 1624 1629
24. D.F. Wallace, I.D. Tonks, A. Zournazi, G.F. Kay, and V.N. Subramaniam Inactivation of the murine Transferrin Receptor 2 gene using the Cre recombinase: loxP system Genesis 39 2004 38 41
25. V.N. Subramaniam, F. Peter, R. Philp, S.H. Wong, and W. Hong GS28, a 28-kilodalton Golgi SNARE that participates in ER-Golgi transport Science 272 1996 1161 1163
26. V.N. Subramaniam, E. Loh, and W. Hong N-Ethylmaleimide-sensitive factor (NSF) and alpha-soluble NSF attachment proteins (SNAP) mediate dissociation of GS28-syntaxin 5 Golgi SNAP receptors (SNARE) complex J Biol Chem 272 1997 25441 25444
27. V.N. Subramaniam, E. Loh, H. Horstmann, A. Habermann, Y. Xu, and J. Coe Preferential association of syntaxin 8 with the early endosome J Cell Sci 113 2000 997 1008
28. H. Horstmann, C.P. Ng, B.L. Tang, and W. Hong Ultrastructural characterization of endoplasmic reticulum-Golgi transport containers (EGTC) J Cell Sci 115 2002 4263 4273
29. K.L. Guan, and J.E. Dixon Eukaryotic proteins expressed in Escherichia coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase Anal Biochem 192 1991 262 267
30. Q. Zeng, V.N. Subramaniam, S.H. Wong, B.L. Tang, R.G. Parton, and S. Rea A novel synaptobrevin/VAMP homologous protein (VAMP5) is increased during in vitro myogenesis and present in the plasma membrane Mol Biol Cell 9 1998 2423 2437
31. G.J. Darlington, H.P. Bernhard, R.A. Miller, and F.H. Ruddle Expression of liver phenotypes in cultured mouse hepatoma cells J Natl Cancer Inst 64 1980 809 819
32. C. Alvarez, H. Fujita, A. Hubbard, and E. Sztul ER to Golgi transport: Requirement for p115 at a pre-Golgi VTC stage J Cell Biol 147 1999 1205 1222
33. B. Courselaud, M.B. Troadec, S. Fruchon, G. Ilyin, N. Borot, and P. Leroyer Strain and gender modulate hepatic hepcidin 1 and 2 mRNA expression in mice Blood Cells Mol Dis 32 2004 283 289
34. D.Q. Lou, G. Nicolas, J.C. Lesbordes, L. Viatte, G. Grimber, and M.F. Szajnert Functional differences between hepcidin 1 and 2 in transgenic mice Blood 103 2004 2816 2821