Intramolecular inhibition of human defensin HNP-1 by its propiece

Journal of Clinical Investigation

Volumn 97, Issue 7, 1996, Pages 1624-1629

  Valore, Erika V ^a   Martin, Edith ^a   Harwig, Sylvia S L ^a   Ganz, Tomas ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

antimicrobial peptide; cytotoxicity; inflammation; neutrophil; posttranslational processing

Indexed keywords

DEFENSIN;

ANTIBACTERIAL ACTIVITY; ARTICLE; CELL STRAIN K 562; CYTOTOXICITY; HUMAN; HUMAN CELL; LISTERIA MONOCYTOGENES; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION;

EID: 0029978338     PISSN: 00219738     EISSN: None     Source Type: Journal    
DOI: 10.1172/JCI118588     Document Type: Article

References (36)

1. Lehrer, R I., A.K. Lichtenstein, and T. Ganz. 1993. Defensis: antimicrobial and cytotoxic peptides of mammalian cells. Annu. Rev. Immunol. 11:105-128
2. Hultmark, D. 1993. Immune reactions in Drosophila and other insects: a model for innate immunity. Trends Genet 9:178-183.
3. Hoffmann, J.A., and C. Hetru. 1992. Insect defensins: inducible antibacterial peptides. Immunol Today. 13:411-415.
4. Cammue, B P., M.F. De Bolle, F.R. Terras, P. Proost, J. Van Damme, S.B. Rees J. Vanderleyden, and W.F. Broekaert. 1992. Isolation and characterization of a novel class of plant antimicrobial peptides form Mirabilis jalapa L. seeds. J. Biol. Chem 267:2228-2233.
5. Aley, S.B , M. Zimmerman, M. Hetsko, M.E. Selsted, and F.D. Gillin. 1994. Killing of Giardia lamblia by cryptdins and cationic neutrophil peptides. Infect. Immun. 62:5397-5403.
6. Daher, K.A., M.E. Selsted, and R.I. Lehrer. 1986. Direct inactivation of viruses by human granulocyte defensins. J. Virol. 60:1068-1074.
7. Okrent, D.G., A.K. Lichtenstein, and T. Ganz. 1990. Direct cytotoxicity of polymorphonuclear leukocyte granule proteins to human lung-derived cells and endothelial cells. Am. Rev. Respir. Dis 141:179-185.
8. Lichtenstein, A., T. Ganz, M.E Selsted, and R.I. Lehrer. 1986 In vitro tumor cell cytolysis mediated by peptide defensins of human and rabbit granulocytes. Blood. 68:1407-1410.
9. Lehrer, R.I., A. Barton, K.A. Daher, S.S. Harwig, T. Ganz, and M.E. Selsted. 1989. Interaction of human defensins with Escherichia coli. Mechanism of bactericidal activity. J. Clin. Invest. 84:553-561.
10. Lichtenstein, A. 1991. Mechanism of mammalian cell lysis mediated by peptide defensins. Evidence for an initial alteration of the plasma membrane. J. Clin. Invest. 88:93-100.
11. Valore, E.V., and T. Ganz. 1992. Posttranslational processing of defensins in immature human myeloid cells. Blood. 79.1538-1544.
12. Liu, L., and T. Ganz. 1995. The pro region of human neutrophil defensin contains a motif that is essential for normal subcellular sorting. Blood. 85:1095-1103.
13. Michaelson, D., J. Rayner, M. Couto, and T. Ganz. 1992 Cationic defensins arise from charge-neutralized propeptides: a mechanism for avoiding leukocyte autocytotoxicity? J. Leukocyte Biol 51:634-639.
14. Dagert, M., and S. D. Ehrlich. 1979. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene (Amst.) 6: 23-28.
15. Panyutich, A.V., N.N. Voitenok, R.I. Lehrer, and T. Ganz. 1991. An enzyme immunoassay for human defensins. J. Immunol. Methods 141:149-155.
16. Harwig, S.S., N.P. Chen, A.S. Park, and R.I. Lehrer. 1993. Purification of cysteine-rich bioactive peptides from leukocytes by continuous acid-ureapolyacrylamide gel electrophoresis. Anal. Biochem. 208.382-386.
17. Schagger, H., and G. von Jagow. 1987. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166:368-379.
18. Villa, S., G. De Fazio, and U. Canosi, 1989. Cyanogen bromide cleavage at methionine residues of polypeptides containing disulfide bonds. Anal. Biochem. 177:161-164.
19. Lehrer, R.I., M. Rosenman, S.S. Harwig, R. Jackson, and P. Eisenhauer. 1991. Ultrasensitive assays for endogenous antimicrobial polypeptides. J. Immunol. Methods. 137:167-173.
20. Daher, K.A., R.I. Lehrer, T. Ganz, and M. Kronenberg. 1988. Isolation and characterization of human defensin cDNA clones. Proc. Natl. Acad. Sci. USA. 85:7327-7331.
21. Ganz, T., M.E. Selsted, D. Szklarek, S.S. Harwig K. Daher. D.F. Bainton, and R.I. Lehrer. 1985. Defensins Natural peptide antibiotics of human neutrophils. J. Clin. Invest. 76:1427-1435.
22. Piers, K.L., M.H. Brown, and R.E. Hancock. 1993. Recombinant DNA procedures for producing small antimicrobial cationic peptides in bacteria. Gene (Amst.). 134:7-13.
23. Baker, D., J.L. Silen, and D.A. Agard. 1992. Protease pro region required for folding is a potent inhibitor of the mature enzyme. Proteins. 12:339-344.
24. Baker, D., A.K. Shiau, and D A. Agard. 1993. The role of pro regions in protein folding. Curr. Optn. Cell Biol 5:966-970.
25. Stoller, T.J., and D. Shields. 1989. The propeptide of preprosomatostatin mediates intracellular transport and secretion of alpha-globin from mammalian cells. J Cell Biol. 108:1647-1655.
26. James, M.N., and A.R. Sielecki. 1986. Molecular structure of an aspartic proteinasc zymogen. porcine pepsinogen, at 1.8 A resolution Nature (Lond.), 319:33-38.
27. Lehrer, R.I., A. Barton, and T. Ganz. 1988 Concurrent assessment of inner and outer membrane perineabilization and bacteriolysis in E. coli by multiple-wavelength spectrophotometry. J. Immunol. Methods. 108:153-158.
28. Kagan, B.L., M.E. Selsted, T. Ganz, and R.I. Lehrer. 1990. Antimicrobial defensin peptides form voltage-dependent ion-permeable channels in planar lipid bilayer membranes. Proc. Natl. Acad. Sci. USA. 87.210-214.
29. Wimley, W.C., M.E. Selsted, and S.H. White. 1994 Interactions between human defensins and lipid bilayers: evidence for formation of multimeric pores. Protein Sci. 3:1362-1373.
30. Barker, R L., R.H. Gundel, G.J. Gleich, J.L. Checkel, D.A. Loegering, L.R. Pease, and K.J Hamann 1991. Acidic polyamino acids inhibit human eosinophil granule major basic protein toxicity. Evidence of a functional role of proMBP. J. Clin. Invest. 88:798-805.
31. Bainton, D.F., J.L. Ullyot, and M.G. Farquhar. 1971. The development of neutrophilic polymorphonuclear leukocytes in human bone marrow. J. Exp. Med 134:907-934.
32. Rice, W G., T. Ganz, J.M. Kinkade, M.E. Selsted, R.I. Lehrer, and R.T. Parmley. 1987. Defensin-rich dense granules of human neutrophils. Blood. 70: 757-765.
33. Joiner, K.A., T. Ganz, J. Albert, and D. Rotrosen. 1989. The opsonizing ligand on Salmonella typhimurrium influences incorporation of specific, but not azurophil, granule constituents into neutrophil phagosomes J. Cell Biol 109: 2771-2782.
34. Leffell, M.S., and J.K. Spitznagel 1974. Intracellular and extracellular degranulation of human polymorphonuclear azurophil and specific granules induced by immune complexes. Infect. Immun. 10.1241-1249.
35. Ganz, T. 1987. Extracellular release of antimicrobial defensins by human polymorphonuclear leukocytes. Infect. Immun. 55:568-571
36. Ganz, T., L. Liu, E.V. Valore, and A. Oren. 1993. Posttranslational processing and targeting of transgenic human defensin in murine granulocyte, macrophage, fibroblast, and pituitary adenoma cell lines. Blood. 82:641-650.