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Volumn 272, Issue 5265, 1996, Pages 1161-1163

GS28, a 28-kilodalton golgi SNARE that participates in ER-Golgi transport

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BIOASSAY; BIOCHEMISTRY; CELL MEMBRANES; DNA; DNA SEQUENCES; POLYPEPTIDES;

EID: 15844394630     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.272.5265.1161     Document Type: Article
Times cited : (125)

References (27)
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    • note
    • Membrane proteins extracted from salt-washed rat liver Golgi membranes (6) were incubated with CNBr-Sepharose conjugated with mAb HFD9. p28 eluted from the beads was subjected to trypsin or endoproteinase GluC digestion. The resulting peptides were sequenced with a model 6600 ProSequencer (Milligen, Burlington, MA). One cDNA fragment of about 400 bp was obtained by PCR with a sense oligonucleotide [GA(T/C)AT(T/C/A)(T/C)TI-CA(A/G)GA(T/C)TA(T/C)ACICA] to peptide P4 (DILDYQTHE) and an antisense oligonucleotide [TG(A/G/T)ATIA(A/G)I(C/G)(A/T)(A/G)TTIACAGCIGG (A/G)AA] to peptide P9 (FPAVNSLIQR). This cDNA fragment was used to isolate a full-length rat cDNA clone (SKT7p28). Database searches were done with the BLAST program.
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    • note
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    • 6-α-SNAP in a final volume of 500 μl. After a 30-min incubation at 4°C, the samples were loaded onto a 15 to 40% (w/v) glycerol gradient in the assembly (Flj. 4A) (a and b) or the disassembly buffer (c). Centrifugation was carried out for 18 hours in a SW41 rotor (Beckman). Fractions of about 0.8 ml were collected manually from the bottom at a flow rate of ∼1 ml/min. Samples were resolved by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), and the distribution of p28 was determined by immunoblotting.
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    • note
    • We thank J. E. Rothman and W. E. Balch for re-agents, C. Pallen, W. Chia, and members of W. Hong's laboratory for critical reading of the manuscript, and Y. H. Tan for his continuous support.


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